We narrowed to 26,837 results for: tat

1. pBEL1954

   Plasmid

   #195689

   Purpose

   Expression/purification of 6xHis-TEV-MlaD(F118A/L123A)

   Depositor

   Damian Ekiert, Gira Bhabha

   Article

   MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

   Insert

   MlaD
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   6xHis-TEV-MlaD(F118A/L123A)

   Promoter

   Available since

   May 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pBEL2319

   Plasmid

   #195697

   Purpose

   Cryo-EM of 6xHis-Foldon-(Gly-Ser-Linker)-MlaC

   Depositor

   Damian Ekiert, Gira Bhabha

   Article

   MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

   Insert

   Foldon-MlaC
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   6xHis-Foldon-(Gly-Ser-Linker)-MlaC

   Promoter

   Available since

   May 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBEL2707

   Plasmid

   #195636

   Purpose

   Expression/purification of 6xHis-TEV-sfGFP-MlaA (227-251)

   Depositor

   Damian Ekiert, Gira Bhabha

   Article

   MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

   Insert

   sfGFP-MlaA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   6xHis-TEV-sfGFP-MlaA (227-251)

   Promoter

   Available since

   May 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBEL2753

   Plasmid

   #195637

   Purpose

   Expression/purification of 6xHis-TEV-sfGFP-MlaA (227-251; I241N)

   Depositor

   Damian Ekiert, Gira Bhabha

   Article

   MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

   Insert

   sfGFP-MlaA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   6xHis-TEV-sfGFP-MlaA (227-251; I241N)

   Promoter

   Available since

   May 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBEL2754

   Plasmid

   #195638

   Purpose

   Expression/purification of 6xHis-TEV-sfGFP-MlaA (227-251; L245N)

   Depositor

   Damian Ekiert, Gira Bhabha

   Article

   MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

   Insert

   sfGFP-MlaA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   6xHis-TEV-sfGFP-MlaA (227-251; L245N)

   Promoter

   Available since

   May 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBEL2755

   Plasmid

   #195639

   Purpose

   Expression/purification of 6xHis-TEV-sfGFP-MlaA (227-251; I248N)

   Depositor

   Damian Ekiert, Gira Bhabha

   Article

   MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

   Insert

   sfGFP-MlaA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   6xHis-TEV-sfGFP-MlaA (227-251; I248N)

   Promoter

   Available since

   May 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBEL2756

   Plasmid

   #195640

   Purpose

   Expression/purification of 6xHis-TEV-sfGFP-MlaA(227-251; delta238-251)

   Depositor

   Damian Ekiert, Gira Bhabha

   Article

   MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

   Insert

   sfGFP-MlaA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   6xHis-TEV-sfGFP-MlaA(227-251; delta238-251)

   Promoter

   Available since

   May 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBEL2757

   Plasmid

   #195641

   Purpose

   Expression/purification of 6xHis-TEV-sfGFP-MlaA(227-251; delta244-251)

   Depositor

   Damian Ekiert, Gira Bhabha

   Article

   MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

   Insert

   sfGFP-MlaA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   6xHis-TEV-sfGFP-MlaA(227-251; delta244-251)

   Promoter

   Available since

   May 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. H1.224.M.luc

   Plasmid

   #193705

   Purpose

   Expresses a truncated PolIII H1 promoter of 224 nucleotides (H1.224) with the TATA box mutated

   Depositor

   Elena Herrera-Carrillo

   Article

   Gao et al J Biol Chem. 2020 Nov 5. pii: S0021-9258

   Insert

   Truncated PolIII H1 promoter (H1.224) with the TATA box mutated
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCRISPR2_-6D

    Plasmid

    #149387

    Purpose

    P. aeruginosa PA14 CRISPR2 locus, with 6bp deletion downstream of IHFprox site

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

    Insert

    Pseudomonas aeruginosa PA14 CRISPR2 locus
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    6bp deleted downstream of the IHFprox site

    Promoter

    pBAD

    Available since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCRISPR2_-5D

    Plasmid

    #149388

    Purpose

    P. aeruginosa PA14 CRISPR2 locus, with 5bp deletion downstream of IHFprox site

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

    Insert

    Pseudomonas aeruginosa PA14 CRISPR2 locus
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    5bp deleted downstream of the IHFprox site

    Promoter

    pBAD

    Available since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCRISPR2_+5D

    Plasmid

    #149390

    Purpose

    P. aeruginosa PA14 CRISPR2 locus, with 5bp addition downstream of IHFprox site

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

    Insert

    Pseudomonas aeruginosa PA14 CRISPR2 locus
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    5bp inserted downstream of the IHFprox site

    Promoter

    pBAD

    Available since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCRISPR2_+10D

    Plasmid

    #149391

    Purpose

    P. aeruginosa PA14 CRISPR2 locus, with 10bp addition downstream of IHFprox site

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

    Insert

    Pseudomonas aeruginosa PA14 CRISPR2 locus
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    10bp inserted downstream of the IHFprox site

    Promoter

    pBAD

    Available since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCRISPR2_IHF_Opt

    Plasmid

    #149395

    Purpose

    P. aeruginosa PA14 CRISPR2 locus, with "optimized" IHFprox site

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

    Insert

    Pseudomonas aeruginosa PA14 CRISPR2 locus
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    IHFprox site is optimized at key positions needed…

    Promoter

    pBAD

    Available since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCRISPR2_motif_scram

    Plasmid

    #149397

    Purpose

    P. aeruginosa PA14 CRISPR2 locus, with a scrambled Upstream recognition motif

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

    Insert

    Pseudomonas aeruginosa PA14 CRISPR2 locus
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Upstream motif recognized by Cas1-2/3 during spac…

    Promoter

    pBAD

    Available since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pWSK129-stm2585_S130A

    Plasmid

    #174403

    Purpose

    Bacterial expression of sarA with its GSK3-binding SxxxSP motif mutated to SxxxAP.

    Depositor

    Dennis Ko

    Article

    Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

    Insert

    sarA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    sarA with S130A mutation

    Promoter

    Available since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. sg3

    Plasmid

    #113968

    Purpose

    Single short guide RNA targeting CAATGTATCTTATCATGTC

    Depositor

    Jordan Green

    Article

    Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

    Insert

    sg3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. sg2+sg3

    Plasmid

    #113970

    Purpose

    Double short guide RNA targeting TACCACATTTGTAGAGGTT & CAATGTATCTTATCATGTC

    Depositor

    Jordan Green

    Article

    Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

    Insert

    sg2+sg3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGI3EM11

    Plasmid

    #135483

    Purpose

    Agrobacterium T-DNA with SpunH2Bpr driving hph-GFP

    Depositor

    Nicolas Buchler

    Article

    Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

    Insert

    SpunH2Bpr hph-GFP ADH1t
    

    Use

    Tags

    GFP

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    SpunH2Bpr

    Available since

    May 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGI3EM29

    Plasmid

    #135489

    Purpose

    Agrobacterium T-DNA with Spun H2Bpr driving hph-mCitrine

    Depositor

    Nicolas Buchler

    Article

    Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

    Insert

    hph SpunH2A/Bpr hph-mCitrine
    

    Use

    Tags

    mCitrine

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    SpunH2A/Bpr (divergent)

    Available since

    May 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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