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Plasmid#212742PurposeConsitutive expression of NUbo-E3(48)-VSV in budding yeastDepositorInsertE3(48)
UseIntegrative vectorTagsNUbo and VSVExpressionYeastMutationPromoterADH1Available sinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp128-ADH-NUbo-E3(48)-VSV
Plasmid#212741PurposeConsitutive expression of NUbo-E3(48)-VSV in budding yeastDepositorInsertE3(48)
UseIntegrative vectorTagsVSVExpressionYeastMutationPromoterADH1Available sinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp211-ADH-CUbo-VSV
Plasmid#212733PurposeConsitutive expression of CUbo-VSV in budding yeastDepositorInsertCUbo
UseIntegrative vectorTagsVSVExpressionYeastMutationPromoterADH1Available sinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp128-ADH-CUbo-VSV
Plasmid#212732PurposeConsitutive expression of CUbo-VSV in budding yeastDepositorInsertCUbo
UseIntegrative vectorTagsVSVExpressionYeastMutationPromoterADH1Available sinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp211-CUP1-NUbo-VSV
Plasmid#212730PurposeCopper-inducible expression of NUbo-VSV in budding yeastDepositorInsertNUbo
UseIntegrative vectorTagsVSVExpressionYeastMutationPromoterCUP1Available sinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp211-ADH-NUbo-VSV
Plasmid#212729PurposeConsitutive expression of NUbo-VSV in budding yeastDepositorInsertNUbo
UseIntegrative vectorTagsVSVExpressionYeastMutationPromoterADH1Available sinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp128-ADH-NUbo-VSV
Plasmid#212728PurposeConsitutive expression of NUbo-VSV in budding yeastDepositorInsertNUbo
UseIntegrative vectorTagsVSVExpressionYeastMutationPromoterADH1Available sinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-NUbo-E3(48)-FLAG
Plasmid#212802PurposeConstitutive/Doxycycline-inducible expression of NUbo-E3(48)-FLAG in mammalian cellsDepositorInsertE3(48)
UseTagsFLAG and NUboExpressionMammalianMutationPromoterAvailable sinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST-3xFLAG-Ubc7
Plasmid#212801PurposeConstitutive expression of 3xFLAG-tagged budding yeast Ubc7 in mammalian cellsDepositorInsertUbc7
UseTags3xFLAGExpressionMammalianMutationPromoterAvailable sinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-Myc-E3(1)-CUbo
Plasmid#212799PurposeConstitutive/Doxycycline-inducible expression of Myc-E3(1)-CUbo in mammalian cellsDepositorInsertE3(1)
UseTagsCUbo and MycExpressionMammalianMutationPromoterAvailable sinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-Ubc13
Plasmid#212806PurposeConstitutive or doxycycline-inducible expression of budding yeast Ubc13 in mammalian cellsDepositorInsertUbc13
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-Mms2
Plasmid#212807PurposeConstitutive or doxycycline-inducible expression of budding yeast Mms2 in mammalian cellsDepositorInsertMms2
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET24a-His6-CUbo(K63R)-MCS
Plasmid#212727PurposeBacterial expression of a protein of interest with N-terminal His6-CUbo(K63R)-fusion, contains multiple cloning siteDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET24a-His6-CUbo(K48R)-MCS
Plasmid#212726PurposeBacterial expression of a protein of interest with N-terminal His6-CUbo(K48R)-fusion, contains multiple cloning siteDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET15b-His6-CUbo(K63R)-GFP
Plasmid#212722PurposeBacterial expression of His6-CUbo(K63R)-GFPDepositorInsertGFP
UseTags6xHis-CUbo(K63R)ExpressionBacterialMutationPromoterAvailable sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET15b-His6-CUbo(K48R)-GFP
Plasmid#212721PurposeBacterial expression of His6-CUbo(K48R)-GFPDepositorInsertGFP
UseTags6xHis-CUbo(K48R)ExpressionBacterialMutationPromoterAvailable sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET15b-His6-CUbo-GFP
Plasmid#212720PurposeBacterial expression of His6-CUbo-GFPDepositorInsertGFP
UseTags6xHis-CUboExpressionBacterialMutationPromoterAvailable sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET24a-His7-NUbo-GFP
Plasmid#212719PurposeBacterial expression of His7-NUbo-GFPDepositorInsertGFP
UseTags7xHis-NUboExpressionBacterialMutationPromoterAvailable sinceJan. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET24a-NUbo-GFP-His6
Plasmid#212718PurposeBacterial expression of NUbo-GFP-His6DepositorInsertGFP
UseTags6xHis and NUboExpressionBacterialMutationPromoterAvailable sinceJan. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET30-His6-TEV-Mms2
Plasmid#212716PurposeBacterial expression of His6-Mms2, His6-tag cleavable with TEV-proteaseDepositorInsertMms2
UseTags6xHis-TEVExpressionBacterialMutationPromoterAvailable sinceJan. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMAL-TEV2-Ubc7-His6
Plasmid#212713PurposeBacterial expression of MBP-Ubc7-His6, MBP-tag cleavable with TEV-proteaseDepositorInsertUbc7
UseTags6xHis and MBP-TEV2ExpressionBacterialMutationPromoterAvailable sinceJan. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pENTR2b-mScarlet
Plasmid#207961PurposeGateway entry vector with the mScarlet fluorescent proteinDepositorInsertmScarlet
UseGateway entry vectorTagsExpressionMutationPromoterNoneAvailable sinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOP004
Plasmid#185985PurposeEncodes a 0.8 kb bacterial transcription unit with Broccoli-tRNA fluorogenic aptamer shortly upstream of the intrinsic terminatorDepositorInsertBacterial transcription unit encoding Broccoli-tRNA fluorogenic aptamer
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOP005
Plasmid#186030PurposeEncodes a 0.8 kb bacterial transcription unit with Broccoli-tRNA fluorogenic aptamer shortly downstream of the promoterDepositorInsertBacterial transcription unit encoding Broccoli-tRNA fluorogenic aptamer
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDR111_mannose_Bgal NLS
Plasmid#188398PurposeExpresses beta-galactosidase by D-mannose promoter for Bacillus subtilis. There is a secretion peptide (PhoD) and nuclear localization signal (SV40) that is fused to beta-galactosidase.DepositorInsertlacZ
UseTagsPhoD secretion peptide and SV40 NLSExpressionBacterialMutationPromoterD-mannoseAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV.U6gLacZ.EFS-NS.H2B-RFP
Plasmid#170363PurposeNegative control plasmid used for Crispr/cas9 based disruption. Expressing nuclear RFP.DepositorInsertH2B-RFP
UseLentiviralTagsExpressionMutationPromoterEFS-NSAvailable sinceJune 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
Tol2-aACry::EGFP-zebrafish Plaat1 (WT)
Plasmid#168772PurposeExpress EGFP-zebrafish Plaat1 (WT) in zebrafish lensDepositorInsertHrasls (plaat1 Zebrafish)
UseTagsEGFPExpressionMutationPromoteralphaA-crystallin promoterAvailable sinceMay 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMIII-Neo-mChe
Plasmid#153423PurposeRetroviral (MSCV) expression of mCherry fluorescent proteinDepositorInsertmCherry
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSC45
Plasmid#104819PurposeCRISPR/Cas9 1xplex gRNA targeting Glyma.04g057400 (Dcl3a-2). Also expresses Cas9 from Gmubi promoter.DepositorInsertGlyma.04g057400
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSC29
Plasmid#104803PurposeCRISPR/Cas9 2xplex gRNA targeting Medtr4g094545 (Hen1). Also expresses Cas9 from Gmubi promoter.DepositorInsertMedtr4g094545
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits only