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Plasmid#175578PurposeDNA donor for transpositionDepositorInsertMini-Tn, GFP cassette
UseTagsExpressionBacterialMutationNOPromoterAvailable sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTns(Ptr)
Plasmid#175576PurposeExpression of CRISPR-associated transposasesDepositorInsertPtrTnsABC
UseTagsExpressionBacterialMutationNOPromoterAvailable sinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKB817 (pBra-Hfq)
Plasmid#174661PurposePlpp/PlacUV5- directed synthesis of the alpha NTD fused to E. coli Hfq. Useful as three-hybrid positive control.DepositorInsertHfq
UseTagsalpha NTDExpressionBacterialMutationPromoterlpp/lacUV5 (tandem promoter)Available sinceMay 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDonorCm(Ptr)
Plasmid#175577PurposeDNA donor for transpositionDepositorInsertMini-Tn, Cm cassette
UseTagsExpressionBacterialMutationNOPromoterAvailable sinceNov. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMUH_unc84_tdTFlag
Plasmid#46024DepositorInsertUNC-84 (unc-84 )
Use10xuas-phi31-expression vectorTagstdTomatoExpressionMutationTwo amino acid mutations (F279L and L500P) compar…Promoter10XUASAvailable sinceSept. 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV-UbC-mCherry Rab5
Plasmid#115239PurposeTo express in mammalian cells the endocytic protein Rab5 fused to mCherry by transfection or by generating AAV virions.DepositorInsertRab5a (RAB5A Homo sapiens, Human)
UseAAVTagsmCherryExpressionMammalianMutationPromoterHuman UbCAvailable sinceSept. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCH1 - pBait-empty
Plasmid#174663Purposearabinose-induced synthesis of an RNA containing a single MS2 hairpin. Used as a three-hybrid control. For cloning purposes, see plasmid #174662.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMay 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-UbC-eGFP-F
Plasmid#71545PurposeExpresses a membrane bound eGFP, under the UbC promoter. This promoter expresses transgenes in neurons at higher levels than plasmids with the CMV or β-actin-CMV promoters.DepositorInserteGFP-F
UseAAVTagscontains 20- amino-acid farnesylation signal from…ExpressionMammalianMutationPromoterUbCAvailable sinceDec. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pBWB294
Plasmid#140636PurposepBAV1k-lacI-Trc-BCD9-mCherry. BCD RBS.DepositorInsertmCherry
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
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LiRTX_ctrl
Plasmid#214077PurposeLuminescent reporter system, NMD negative control. Intron present in 5'UTRDepositorInsertFirefly luciferase
UseTagsExpressionMammalianMutationRemnant of linker sequence present at the end of …PromoterAvailable sinceFeb. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pL2-GGA(EAAAK)2AGG
Plasmid#137104PurposeiFLinkC Linker cloningDepositorInsertGGA(EAAAK)2AGG
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
Halo-Sox13
Plasmid#200194PurposeExpression of full length Sox13DepositorInsertHalo-Sox13 (Sox13 Mouse)
UseTagsHaloExpressionMammalianMutationNonePromoterEF_1alphaAvailable sinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYZ714
Plasmid#132955PurposecrRNA entry vector of Pfba1-BsaI pad-TEF1 Term with dFnCas12a-Clr4 in Schizosaccharomyces pombeDepositorInsertdFnCas12a
UseCRISPR and Synthetic BiologyTagsSV40 NLS and nucleoplasmin NLSExpressionMutationPromoteradh1Available sinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pECNUS_MP01
Plasmid#194350PurposeCRISPR-Cas9 multiplexing acceptor clone with BpiI cutterDepositorInsertpcoCas9, FAST-Red
UseTagsExpressionPlantMutationPromoterRPS5AAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRRL-GFPGO-PGK-Neo
Plasmid#136890PurposeLentivirus for base editing activatable GFP expression in mammalian cellsDepositorInsertGFPGO
UseLentiviralTagsExpressionMutationATG>ACGPromoterSFFVAvailable sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
LRT2B-AdGO
Plasmid#136910PurposeLentivirus expressing AdGO sgRNADepositorInsertsgAdGO
UseLentiviralTagsExpressionMutationWTPromoterhU6Available sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNUT1
Plasmid#175746PurposeSingle-component photo-inducible (LOV2-based) nucleolus-targeting tool 1DepositorInsertmCherry-LOV2-NoLS (HIV Rev transactivating protein)
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only