We narrowed to 30,488 results for: ica

1. hsa-cdk2 3'UTR sicheck2

   Plasmid

   #121125

   Purpose

   luciferase assay

   Depositor

   Qing Deng

   Article

   Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

   Insert

   hsa-cdk2 3'UTR (CDK2 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRSFDuet1-Cdc45 (delta 154–164)

   Plasmid

   #126879

   Purpose

   Expresses crystallisation construct of human Cdc45 in bacteria

   Depositor

   Luca Pellegrini

   Article

   Simon et al Nat Commun. 2016 May 18;7:11638. doi: 10

   Insert

   Human Cdc45 (CDC45 Human)
   

   Use

   Tags

   No tag

   Expression

   Bacterial

   Mutation

   deletion amino acid 154-164

   Promoter

   T7 promoter

   Available since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUC19_4xT790_28bp

   Plasmid

   #114720

   Purpose

   4 x 28 bp repeats of the EGFR WT allele used as SiMREPS analyte standard

   Depositor

   Nils Walter

   Article

   Hayward et al J Am Chem Soc. 2018 Aug 20. doi: 10.1021

   Insert

   EGFR (EGFR Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUC19_T790_28bp

   Plasmid

   #114722

   Purpose

   28 bp fragment of the WT EGFR allele used as SiMREPS analyte standard

   Depositor

   Nils Walter

   Article

   Hayward et al J Am Chem Soc. 2018 Aug 20. doi: 10.1021

   Insert

   EGFR (EGFR Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGS_N_Strep

   Plasmid

   #101651

   Purpose

   Expression of type I signal-peptide containing proteins with N-terminal Twin-Strep-tag in insect cells

   Depositor

   Monika Golas, Bjoern Sander

   Article

   Le et al Mol Biotechnol. 2018 Jan;60(1):31-40. do

   Insert

   LRP6 Signal peptide, KpnI linker, Twin-Strep tag
   

   Use

   Tags

   Twin-Strep tag

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGS_C_Strep

   Plasmid

   #101652

   Purpose

   Expression of type I signal-peptide containing proteins with C-terminal Twin-Strep-tag in insect cells

   Depositor

   Monika Golas, Bjoern Sander

   Article

   Le et al Mol Biotechnol. 2018 Jan;60(1):31-40. do

   Insert

   LRP6 Signal peptide, MCS linker, Twin-Strep tag
   

   Use

   Tags

   Twin-Strep tag

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. LLhT-pHG165a V52A

   Plasmid

   #90050

   Purpose

   Constitutive expression of a chimeric LacI:TreR transcription repressor

   Depositor

   Liskin Swint-Kruse

   Article

   Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

   Insert

   LLhT/V52A
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   V52A

   Promoter

   Iq

   Available since

   June 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. LLhA-pHG165a Q55A

   Plasmid

   #90052

   Purpose

   Constitutive expression of a chimeric LacI:AscG transcription repressor

   Depositor

   Liskin Swint-Kruse

   Article

   Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

   Insert

   LLhA/Q55A
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Q55A

   Promoter

   Iq

   Available since

   June 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. LLhC-pHG165a Q55I

   Plasmid

   #90053

   Purpose

   Constitutive expression of a chimeric LacI:CytR transcription repressor

   Depositor

   Liskin Swint-Kruse

   Article

   Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

   Insert

   LLhC/Q55I
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Q55I

   Promoter

   Iq

   Available since

   June 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. CC1

    Plasmid

    #63713

    Purpose

    Wild-type voltage sensing domain from Ciona voltage sensing phosphatase fused to super ecliptic A227D with restriction site flanking each transmembrane domain

    Depositor

    Bradley Baker

    Article

    Piao et al J Neurosci. 2015 Jan 7;35(1):372-85. doi

    Insert

    CC1
    

    Use

    Tags

    Super ecliptic pHlorin A227D

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 27, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pcDNA4-rB90

    Plasmid

    #18959

    Depositor

    Mariano Viapiano

    Article

    Viapiano et al J Neurooncol. 2008 Jul . 88(3):261-72.

    Insert

    Brevican C-terminus (Bcan Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Rat brevican C-terminus: Signal peptide aa 1-22 …

    Promoter

    Available since

    Dec. 12, 2008

    Availability

    Academic Institutions and Nonprofits only

12. DCT.VV.Orf46

    Plasmid

    #12734

    Purpose

    This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

    Depositor

    Jack Bennink, Jon Yewdell

    Article

    Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

    Insert

    VV.Orf46
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 1, 2006

    Availability

    Academic Institutions and Nonprofits only

13. pBpaRS3tRNA

    Plasmid

    #197102

    Purpose

    To express the p-benzoylphenylalanine-specific variant of Methanocaldococcus jannaschii TyrRS and its cognate amber suppressor tRNA and allow UAG codon to be translated into p-benzoylphenylalanine.

    Depositor

    Kensaku Sakamoto

    Article

    Sato et al Biochemistry. 2011 Jan 18;50(2):250-7. d

    Insert

    TyrRS variant, amber suppressor tRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Gly32, Ser107, Thr158, Ser159, Arg286 (TyrRS)

    Promoter

    Available since

    June 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-hSyn-DIO-jGCaMP8s-P2A-ChrimsonR-ST

    Plasmid

    #174007

    Purpose

    Cre dependent co-expression of jGCaMP8s and soma-targeted ChrimsonR under the control of an hSyn promoter. jGCaMP8s and ChrimsonR are separated by cleavable peptide sequence P2A.

    Depositor

    Mark Histed

    Article

    LaFosse et al eNeuro. 2023 Mar 29;10(3):ENEURO.0378-22

    Has Service

    AAV9

    Insert

    jGCaMP8s-P2A-ChrimsonR-ST
    

    Use

    AAV

    Tags

    6xHis and soma targeting motif

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGP-CMV-jGCaMP8m

    Plasmid

    #162372

    Purpose

    Mammalian expression of ultrafast protein calcium sensor

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Insert

    jGCaMP8m
    

    Use

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    A25G F286Y

    Promoter

    CMV

    Available since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGP-CMV-jGCaMP8s

    Plasmid

    #162371

    Purpose

    Mammalian expression of ultrafast protein calcium sensor

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Insert

    jGCaMP8s
    

    Use

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    S26M F286Y Q315H

    Promoter

    CMV

    Available since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGP-CMV-jGCaMP8f

    Plasmid

    #162373

    Purpose

    Mammalian expression of ultrafast protein calcium sensor

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Insert

    jGCaMP8f
    

    Use

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Q315L

    Promoter

    CMV

    Available since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. phCMV-GALV-MTR

    Plasmid

    #163612

    Purpose

    Retro and Lentiviral transduction of primary human germinal center B cells

    Depositor

    Daniel Hodson

    Article

    Caeser et al Nat Commun. 2019 Oct 4;10(1):4543. doi:

    Insert

    GaLV MTR envelope
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRK5-HA-Ubiquitin-K48R

    Plasmid

    #17604

    Purpose

    Mammalian expression of HA tagged ubiquitin K48R

    Depositor

    Ted Dawson

    Article

    Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

    Insert

    Ubiquitin C (UBC Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K48R (expected to disrupt G76-K48 ubiquitin chain…

    Promoter

    Available since

    March 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-lyso-pHluorin

    Plasmid

    #70113

    Purpose

    Expression of lyso-pHluorin in mammalian cells for optical readout of lysosomal acidification

    Depositor

    Christian Rosenmund

    Article

    Rost et al Nat Neurosci. 2015 Nov 9. doi: 10.1038/n

    Insert

    lyso-pHluorin (Cd63 Mouse, Aequorea victoria)
    

    Use

    Tags

    HA-Tag and pHluorin

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAM1

    Plasmid

    #51401

    Purpose

    Expresses truncated version of NLL-MetRS from endogenous MetG promoter

    Depositor

    David Tirrell

    Article

    Mahdavi et al Proc Natl Acad Sci U S A. 2014 Jan 7;111

    Insert

    MetRS (metG E coli)
    

    Use

    Tags

    Expression

    Mutation

    L13N, Y260L, H301L; Please note that a truncated …

    Promoter

    MetG

    Available since

    March 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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22. pAAV-Ef1a-DIO-ChRmine-mScarlet-WPRE

    Plasmid

    #130998

    Purpose

    Double floxed ChRmine-mScarlet under the control of Ef1a promoter

    Depositor

    Karl Deisseroth

    Article

    Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

    Has Service

    AAV1 and AAV5

    Insert

    ChRmine
    

    Use

    AAV

    Tags

    mScarlet

    Expression

    Mutation

    Promoter

    Ef1a

    Available since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. TDG004_pLenti_CMVtight_Kir2.1_CFP

    Plasmid

    #178820

    Purpose

    tet-on expression of an inward-rectifier potassium channel Kir2.1 carrying an CFP tag

    Depositor

    Adam Cohen

    Article

    Tian et al Nat Methods. 2023 Jul;20(7):1082-1094. d

    Insert

    Kir2.1_CFP
    

    Use

    Lentiviral

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. AAV-FLEX-Arch-GFP

    Plasmid

    #22222

    Purpose

    Cre-dependent viral expression of Arch-GFP for neuronal inhibition

    Depositor

    Edward Boyden

    Article

    Chow et al Nature. 2010 Jan 7. 463(7277):98-102.

    Has Service

    AAV1 and AAV9

    Insert

    Arch
    

    Use

    AAV and Cre/Lox; Adeno-associated virus

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    no

    Promoter

    CAG

    Available since

    Jan. 6, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAAV-Ef1a-DIO-ChRmine-mScarlet-Kv2.1-WPRE

    Plasmid

    #130999

    Purpose

    Double floxed soma-targeted ChRmine-mScarlet under the control of Ef1a promoter

    Depositor

    Karl Deisseroth

    Article

    Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

    Insert

    ChRmine
    

    Use

    AAV

    Tags

    mScarlet-Kv2.1

    Expression

    Mutation

    Promoter

    Ef1a

    Available since

    Sept. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pF146 pSOD1WTAcGFP1

    Plasmid

    #26407

    Depositor

    Elizabeth Fisher

    Article

    Stevens et al PLoS One. 2010 . 5(3):e9541.

    Insert

    SOD1 (SOD1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 8, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pRK5-HA-Ubiquitin-K33

    Plasmid

    #17607

    Purpose

    Mammalian expression of HA tagged ubiquitin with K33 only, other lysines mutated to arginines

    Depositor

    Ted Dawson

    Article

    Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

    Insert

    Ubiquitin C (UBC Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K33 only, other lysines mutated to arginines; P19…

    Promoter

    Available since

    March 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAAV-CaMKIIa-GCaMP 6m-p2A-ChRmine-Kv2.1-WPRE

    Plasmid

    #131003

    Purpose

    bicistronic AAV vector to express GCaMP6m and soma-targeted ChRmine under the control of CamKIIa promoter

    Depositor

    Karl Deisseroth

    Article

    Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

    Insert

    ChRmine
    

    Use

    AAV

    Tags

    Kv2.1-HA

    Expression

    Mutation

    Promoter

    CaMKIIa

    Available since

    Sept. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA3.1-Hpep1-betaArrestin2-T2A-mTagBFP2

    Plasmid

    #194680

    Purpose

    CMV driven co-expression of peptide complementing GPCR recorder (beta-arrestin 2 fusion) and mTagBFP2

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    Hpep1-betaArrestin2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pF151 pcDNA3.1(+)SOD1WT

    Plasmid

    #26397

    Depositor

    Elizabeth Fisher

    Article

    Stevens et al PLoS One. 2010 . 5(3):e9541.

    Insert

    SOD1 (SOD1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 23, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge