Skip to main content
Addgene
Showing: 1261 - 1280 of 10573 results

  1. PARP2-c098

    Plasmid
    #216873
    Purpose
    Bacterial expression vector for E. coli producing PARP2 catalytic protein (without Regulatory domain); with N-terminal CFP fusion
    Depositor
    Lari Lehtiö
    Article
    Dhakar et al Sci Rep. 2024 Feb 16;14(1):3875. doi: 10
    Insert
    PARP2 catalytic domain (no Regulatory Domain) (PARP2 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    216873-plasmid-map-sequence-id-434366

    Enlarge

  5. pFD116

    Plasmid
    #124769
    Purpose
    pFD116 carries dcas9 controlled by an aTc-inducible Ptet promoter, a sgRNA controlled constitutively from PpflB S. aureus promoter to clone a guide between two BsaI sites and oriT on pLZ12 vector
    Depositor
    David Bikard
    Article
    Depardieu et al Methods. 2019 Aug 1. pii: S1046-2023(18)
    Inserts
    Ptet
    dcas9
    PpflB sgRNA BsaI
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    124769-plasmid-map-sequence-id-246181

    Enlarge

  8. pLC133

    Plasmid
    #198639
    Purpose
    Expression plasmid for dCas9 in E. coli used in Chip-seq experiments. dCas9 is tagged with a C-terminal 3x FLAG tag. A guide RNA can be cloned using BsaI restriction sites.
    Depositor
    David Bikard
    Article
    Rostain et al Nucleic Acids Res. 2023 Mar 17:gkad170.
    Insert
    dCas9
    Use
    Tags
    3x-FLAG
    Expression
    Bacterial
    Mutation
    Promoter
    pTet
    Available since
    April 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    198639-plasmid-map-sequence-id-390787

    Enlarge

  9. pDest-Sumo

    Plasmid
    #106980
    Purpose
    LR reaction to create expression plasmid. For prokaryotic expression of target protein as N-terminal fusion form with Sumo tag
    Depositor
    Han Choe
    Article
    Nguyen et al Sci Rep. 2017 Nov 23;7(1):16139. doi: 10
    Type
    Empty backbone
    Use
    Tags
    His8-Sumo
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    106980-plasmid-map-sequence-id-204719

    Enlarge

  12. pFD115

    Plasmid
    #124768
    Purpose
    pFD115 carries cas9 controlled by an aTc-inducible Ptet promoter, a sgRNA controlled constitutively from PpflB S. aureus promoter to clone a guide between two BsaI sites and oriT on pLZ12 vector
    Depositor
    David Bikard
    Article
    Depardieu et al Methods. 2019 Aug 1. pii: S1046-2023(18)
    Inserts
    Ptet
    cas9
    term PpflB sgRNA (BsaI)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Ptet
    Available since
    Aug. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    124768-plasmid-map-sequence-id-246185

    Enlarge

Showing: 1261 - 1280 of 10573 results