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ApoE3_mCh-SspB (pBS1144)

Plasmid

#185326

Purpose

For the mammalian expression of the human protein ApoE3 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

ApoE3

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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CD55-CD4d3+4-bio

Plasmid

#73116

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding CD55 with rat CD4d3+4-bio

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

CD55 (CD55 Human)

Use

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 28, 2016

Availability

Academic Institutions and Nonprofits only

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ENDOD1-CD4d3+4-bio

Plasmid

#73114

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding ENDOD1 with rat CD4d3+4-bio

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

ENDOD1 (ENDOD1 Human)

Use

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 28, 2016

Availability

Academic Institutions and Nonprofits only

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pH-eCFP

Plasmid

#176555

Purpose

Expression of eCFP for auxotrophic selection in the absence of histidine

Depositor

Markus Ralser

Article

Yu et al Nat Microbiol. 2022 Apr;7(4):542-555. do

Insert

eCFP

Use

Tags

Expression

Yeast

Mutation

Promoter

TDH1(GAP)

Available since

April 6, 2022

Availability

Academic Institutions and Nonprofits only

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pL-eCFP

Plasmid

#176556

Purpose

Expression of eCFP for auxotrophic selection in the absence of leucine

Depositor

Markus Ralser

Article

Yu et al Nat Microbiol. 2022 Apr;7(4):542-555. do

Insert

eCFP

Use

Tags

Expression

Yeast

Mutation

Promoter

TDH1(GAP)

Available since

April 6, 2022

Availability

Academic Institutions and Nonprofits only

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pU-eCFP

Plasmid

#176557

Purpose

Expression of eCFP for auxotrophic selection in the absence of uracil

Depositor

Markus Ralser

Article

Yu et al Nat Microbiol. 2022 Apr;7(4):542-555. do

Insert

eCFP

Use

Tags

Expression

Yeast

Mutation

Promoter

TDH1(GAP)

Available since

April 6, 2022

Availability

Academic Institutions and Nonprofits only

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AchE-CD4d3+4-bio

Plasmid

#73119

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding AchE with rat CD4d3+4-bio

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

AchE (ACHE Human)

Use

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 29, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV.hSyn.Flex.iGluSnFr.WPRE.SV40

Plasmid

#98931

Purpose

Cre dependent AAV expression of glutamate sensor from human synapsin promoter. ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2013 Feb;10(2):162-70. doi:

Has Service

AAV1, AAV5, and AAV9

Insert

iGluSnFr

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

Jan. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pU-mRuby2

Plasmid

#176549

Purpose

Expression of mRuby2 for auxotrophic selection in the absence of uracil

Depositor

Markus Ralser

Article

Yu et al Nat Microbiol. 2022 Apr;7(4):542-555. do

Insert

yomRuby2

Use

Tags

Expression

Yeast

Mutation

Promoter

TDH1(GAP)

Available since

Oct. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pH-mRuby2

Plasmid

#176547

Purpose

Expression of mRuby2 for auxotrophic selection in the absence of histidine

Depositor

Markus Ralser

Article

Yu et al Nat Microbiol. 2022 Apr;7(4):542-555. do

Insert

yomRuby2

Use

Tags

Expression

Yeast

Mutation

Promoter

TDH1(GAP)

Available since

April 6, 2022

Availability

Academic Institutions and Nonprofits only

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pUHD-AML1-ETO

Plasmid

#12429

Depositor

Dong-Er Zhang

Article

Rhoades et al Blood. 2000 Sep 15. 96(6):2108-15.

Insert

AML1-ETO (RUNX1, RUNX1T1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only
pJW1659

Plasmid

#154314

Purpose

NT Donor-cassette for SapTrap

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

30aa linker::AID*::3xFLAG for NT slot

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

Available since

Aug. 21, 2020

Availability

Academic Institutions and Nonprofits only

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NAR4-CD4d3+4-bio

Plasmid

#73118

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding NAR4 with rat CD4d3+4-bio

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

NAR4 (ART4 Human)

Use

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 29, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
ERMAP-CD4d3+4-bio

Plasmid

#73107

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding ERMAP with rat CD4d3+4-bio

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

ERMAP (ERMAP Human)

Use

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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Galectin3-CD4d3+4-bio

Plasmid

#73128

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding Galectin3 with rat CD4d3+4-bio

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

LGALS3 (LGALS3 Human)

Use

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 29, 2016

Availability

Academic Institutions and Nonprofits only

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AMIGO2-CD4d3+4-bio

Plasmid

#73103

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding AMIGO2 with rat CD4d3+4-bio

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

AMIGO2 (AMIGO2 Human)

Use

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 28, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
S1 1029 CP ABEMax strategy 1

Plasmid

#135357

Purpose

Expression of circular permutant of nSpyCas9 at amino acid position 1029 encoding ABEMax (wildtype Tada monomer coupled with evolved Tada monomer) at the N-terminus

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

ABEMax-nSpyCas9 aa 1029

Use

CRISPR

Tags

Expression

Mammalian

Mutation

WT

Promoter

Available since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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AfrLEA3m_mCh-2xFKBP (pBS1137)

Plasmid

#185321

Purpose

For the mammalian expression of the brine shrimp protein AfrLEA3m attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

AfrLEA3m

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pST_APOE_HUMAN_E4_47-300 (pBS0847)

Plasmid

#185279

Purpose

For the mammalian expression of the human protein APOE_HUMAN_E4_47-300. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

APOE_HUMAN_E4_47-300

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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pST_PvLEA4_repeats_k3_26 (pBS0762)

Plasmid

#185244

Purpose

For the mammalian expression of the sleeping chironomid protein PvLEA4_repeats_k3_26. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

PvLEA4_repeats_k3_26

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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