Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 9,586 results for: Coli

Clear keywords
Showing: 641 - 650 of 9586 results

  3. pBBR1-EcTrpRS(H14)

    Plasmid
    #231131
    Purpose
    Expresses mutant E. coli TrpRS from a derepressed lac promoter for proteome-wide incorporation of tryptophan analogues in E. coli/K. pneumoniae.
    Depositor
    Abhishek Chatterjee
    Article
    Loynd et al Nat Chem Biol. 2025 Oct 2. doi: 10.1038/
    Insert
    E. coli tryptophanyl tRNA synthetase
    Use
    Klebsiella pneumoniae expression
    Expression
    Bacterial
    Mutation
    S8A, V144G, V146C
    Available Since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    231131-plasmid-map-sequence-id-458351

    Enlarge

  4. pBBR1-EcTyrRS(VSMA,D165G)

    Plasmid
    #231132
    Purpose
    Expresses mutant E. coli TyrRS from a derepressed lac promoter for proteome-wide incorporation of tyrosine in E. coli/K. pneumoniae.
    Depositor
    Abhishek Chatterjee
    Article
    Loynd et al Nat Chem Biol. 2025 Oct 2. doi: 10.1038/
    Insert
    E. coli tyrosyl tRNA synthetase
    Use
    Klebsiella pneumoniae expression
    Expression
    Bacterial
    Mutation
    Y37V, D165G, D182S, P183M, L186A, D265R
    Available Since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    231132-plasmid-map-sequence-id-458349

    Enlarge

  6. pNC2

    Plasmid
    #72438
    Purpose
    Produces Escherichia coli N5-carboxyaminoimidazole ribonucleotide mutase (EcPurE1); NCAIR synthetase (EcPurK)
    Depositor
    T. J. Kappock, JoAnne Stubbe
    Article
    Meyer et al Biochemistry. 1999 Mar 9;38(10):3012-8.
    Inserts
    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    Expression
    Bacterial
    Mutation
    Pro64 to Arg; Asp65 to His; Gln206 to Arg
    Promoter
    T7 and n/a
    Available Since
    May 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    72438-plasmid-map-sequence-id-140433

    Enlarge

  7. pAIP4_1JL1

    Plasmid
    #234206
    Purpose
    Heterologous protein expression of ribonuclease HI (RNase HI) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    1JL1 (rnhA Escherichia coli)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234206-plasmid-map-sequence-id-464171

    Enlarge

  8. pJK651

    Plasmid
    #71702
    Purpose
    Produces E. coli thioredoxin reductase with a C-terminal His6 tag (EcTrxBH6)
    Depositor
    T. J. Kappock
    Insert
    thioredoxin reductase
    Tags
    His6
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71702-plasmid-map-sequence-id-131727

    Enlarge

  9. pInt_attP1_tsXis_sacB_kanR

    Plasmid
    #205302
    Purpose
    Integrating plasmid for ORBIT in E. coli with self excising factor (Xis) and sacB for counter selection. Used for markerless modifications.
    Depositor
    Scott Saunders
    Article
    Saunders et al bioRxiv 2023
    Type
    Empty backbone
    Use
    Synthetic Biology
    Available Since
    Aug. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    205302-plasmid-map-sequence-id-404790

    Enlarge

  10. pJK615

    Plasmid
    #71699
    Purpose
    Produces E. coli thioredoxin with a C-terminal His6 tag (EcTrxAH6)
    Depositor
    T. J. Kappock
    Insert
    thioredoxin
    Tags
    His6
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71699-plasmid-map-sequence-id-137034

    Enlarge

Showing: 641 - 650 of 9586 results