We narrowed to 3,182 results for: Ubc

1. pcDNA5-FRT-TO-NUbo-E3(48)-mCherry-P2A-3xFLAG-Ubc7

   Plasmid

   #212805

   Purpose

   Constitutive or doxycycline-inducible expression of NUbo-E3(48)-mCherry-P2A-3xFLAG-Ubc7 in mammalian cells

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Insert

   NUbo-E3(48)-mCherry-P2A-3xFLAG-Ubc7
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-Puro

   Plasmid

   #71236

   Purpose

   Express sgRNA and dCas9-KRAB from lentiviral vector

   Depositor

   Charles Gersbach

   Article

   Thakore et al Nat Methods. 2015 Dec;12(12):1143-9. doi

   Inserts

   humanized dCas9-KRAB T2A Puro
   sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Flag

   Expression

   Mutation

   D10A and H840A

   Promoter

   Available since

   Dec. 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-GFP

   Plasmid

   #71237

   Purpose

   Express sgRNA and dCas9-KRAB from 3rd generation lentiviral vector

   Depositor

   Charles Gersbach

   Article

   Thakore et al Nat Methods. 2015 Dec;12(12):1143-9. doi

   Inserts

   humanized dCas9-KRAB T2A GFP
   sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Flag

   Expression

   Mutation

   D10A and H840A

   Promoter

   Available since

   Dec. 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUbC-FLAG-24xSuntagV4-oxEBFP-AID-baUTR1-24xMS2V5-Wpre

   Plasmid

   #84561

   Purpose

   pSINAPS; single molecule translation reporter containing a FLAG tag, Suntag, oxBFP, AID, beta-actin 3'UTR, and MS2V5 stem-loops

   Depositor

   Robert Singer

   Article

   Wu et al Science. 2016 Jun 17;352(6292):1430-5. d

   Insert

   FLAG tag, Suntag, oxBFP, Auxin-inducible degron, beta-actin 3'UTR, MS2V5 stem-loops
   

   Use

   Lentiviral

   Tags

   Flag, Suntag, oxBFP, AID (auxin-inducible degron)

   Expression

   Mutation

   Promoter

   UbC

   Available since

   Dec. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV hU6-gRNA hUbC-dSaCas9-KRAB-T2A-Thy1.1

   Plasmid

   #194278

   Purpose

   Expresses gRNA and dSaCas9-KRAB from lentiviral vector

   Depositor

   Charles Gersbach

   Article

   McCutcheon et al bioRxiv. 2023 May 1:2023.05.01.538906. d

   Inserts

   humanized dSaCas9 KRAB T2A Thy1.1
   gRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   hU6 and hUbC

   Available since

   Jan. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFastBac cloning vector with BioBrick PolyPromoter LIC Subcloning (438-A)

   Plasmid

   #55218

   Depositor

   Scott Gradia

   Article

   Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   June 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-Cre-MS2_UbC-NLS-tdMCP-GFP (Cre_tdMCP-GFP)

   Plasmid

   #219537

   Purpose

   a. Expresses Cre recombinase tagged with 23x repeats of MS2 binding sequence under EF1A promotor and b. NLS-tdMCP_GFP reporter under UbC promotor

   Depositor

   Jeffrey Gerst

   Article

   Haimovich et al bioRxiv 2024.11.06.622258

   Inserts

   Cre recombinase (cre )
   NLS-tdMCP-GFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 11, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pLTR-SFFV-PTPN2 transcript variant 1-WPRE-UbC-Emerald

   Plasmid

   #201186

   Purpose

   Lentiviral vector plasmid expressing human PTPN2 transcript variant 1 under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

   Depositor

   Bart Nieuwenhuis

   Inserts

   PTPN2, transcript variant 1 (PTPN2 Human)
   Emerald
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLV hU6-sgKRAS hUbC-dCas9-KRAB-T2a-GFP1

   Plasmid

   #184556

   Purpose

   Knockdown of murine KRAS gene by targeting the promoter region via CRISPRi system

   Depositor

   Sandeep Prabhu

   Article

   Qian et al Cell Rep. 2023 Jul 25;42(7):112794. doi:

   Insert

   Kirsten rat sarcoma virus (Kras Mouse)
   

   Use

   CRISPR, Lentiviral, and Mouse Targeting

   Tags

   Flag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV hU6-sgKRAS hUbC-dCas9-KRAB-T2a-GFP2

    Plasmid

    #184557

    Purpose

    Knockdown of murine KRAS gene by targeting the promoter region via CRISPRi system

    Depositor

    Sandeep Prabhu

    Article

    Qian et al Cell Rep. 2023 Jul 25;42(7):112794. doi:

    Insert

    Kirsten rat sarcoma virus (Kras Mouse)
    

    Use

    CRISPR, Lentiviral, and Mouse Targeting

    Tags

    Flag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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