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pEt28a-H6-SUMO-SilkCatcher

Plasmid

#230902

Purpose

Bacterial expression of SilkCatcher

Depositor

Sesilja Aranko

Article

Fan et al Angew Chem Int Ed Engl. 2023 Mar 6;62(11

Insert

H6-SUMO-SilkCatcher

Use

Tags

HisTag, thrombin, SUMO

Expression

Bacterial

Mutation

Promoter

Available since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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pET28a-ImpBeta1-mEosEM

Plasmid

#218933

Purpose

Express ImpBeta1 (human) with mEosEM fused at the C-terminus.

Depositor

Siegfried Musser

Article

Sau et al Nature. 2025 Mar 19. doi: 10.1038/s41586

Insert

Importin Beta (KPNB1 Human)

Use

Tags

6xHis and mEos4b (EosEM)

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 28, 2025

Availability

Academic Institutions and Nonprofits only

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pET28a-Pchi-HMG

Plasmid

#231540

Purpose

Expresses the HMG of Pchi Sox with a 6xHis tag for protein purification

Depositor

Ralf Jauch

Article

Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

Insert

Sox HMG

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

March 18, 2025

Availability

Academic Institutions and Nonprofits only

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pET28a-Salhel-HMG

Plasmid

#231539

Purpose

Expresses the HMG of Salhel Sox-I with a 6xHis tag for protein purification

Depositor

Ralf Jauch

Article

Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

Insert

Sox-I HMG

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

March 18, 2025

Availability

Academic Institutions and Nonprofits only

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pET28a-Myflu-POU

Plasmid

#231542

Purpose

Expresses the DNA binding domain of Myflu POU with a 6xHis tag for protein purification

Depositor

Ralf Jauch

Article

Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

Insert

POU DBD

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

March 18, 2025

Availability

Academic Institutions and Nonprofits only

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pET28a-Salhel-POU

Plasmid

#231541

Purpose

Expresses the DNA binding domain of Salhel POU with a 6xHis tag for protein purification

Depositor

Ralf Jauch

Article

Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

Insert

POU DBD

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

March 18, 2025

Availability

Academic Institutions and Nonprofits only

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pET28-NFHΔ462-638

Plasmid

#232061

Purpose

Expresses construct for surface tethering: R. norvegicus NFH tail domain lacking residues 462-638

Depositor

Sanjay Kumar

Article

Ding et al Proc Natl Acad Sci U S A. 2024 Dec 3;121

Insert

Neurofilament-Heavy tail domain lacking residues 462-638

Use

Tags

6xHis

Expression

Bacterial

Mutation

added N-terminal tryptophan and tyrosine, and cys…

Promoter

Available since

March 5, 2025

Availability

Academic Institutions and Nonprofits only

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pZH82 pET28a-Rai1

Plasmid

#231651

Purpose

Bacterial expression vector expressing yeast S. cerevisiae rai1, no tag

Depositor

Jesper Svejstrup

Article

Han et al Mol Cell. 2023 Sep 21;83(18):3253-3267.e

Insert

rai1 (RAI1 Budding Yeast)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pET28_Strep-Psc-pScaC

Plasmid

#228834

Purpose

E. coli expression of the ScaC passenger domain

Depositor

Andrew Carter

Article

Manigrasso et al bioRxiv 2024.04.11.589026

Insert

ScaC

Use

Tags

Strep

Expression

Bacterial

Mutation

aa 33-223

Promoter

Available since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

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pET28a-OuIAD(E502Q)

Plasmid

#226973

Purpose

Expressing Olsenella uli indoleacetate decarboxylase (IAD) E502Q variant in E. coli

Depositor

Emily Balskus

Article

Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

Insert

OuIAD(E502Q)

Use

Tags

His6 (on backbone)

Expression

Bacterial

Mutation

E502Q

Promoter

Available since

Jan. 17, 2025

Availability

Academic Institutions and Nonprofits only

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