We narrowed to 44,084 results for: cha

1. pAAV-hSyn1-tdTomatof

   Plasmid

   #104060

   Purpose

   Also known as hSyn1-tdTomato-f. AAV expression of Farnylsated tdtomato for tracing

   Depositor

   Viviana Gradinaru

   Article

   Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

   Insert

   tdTomato
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSyn

   Available Since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-GFAP-2xNLS-mTurquoise2

   Plasmid

   #104053

   Purpose

   An AAV genome encoding expression of the nuclear localized fluorescent protein mTurquoise2 from the GFAP promoter

   Depositor

   Viviana Gradinaru

   Article

   Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

   Insert

   2xNLS-mTurquoise2
   

   Use

   AAV

   Tags

   NLS

   Expression

   Mutation

   Promoter

   GFAP

   Available Since

   April 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-TRE-DIO-eYFP-f

   Plasmid

   #104057

   Purpose

   An AAV genome with tet-inducible, Cre-dependent expression of the fluorescent protein eYFP with a C-terminal Hras farnesylation sequence

   Depositor

   Viviana Gradinaru

   Article

   Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

   Insert

   EYFP-f
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   TRE

   Available Since

   June 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET28a(+)-HisTim12_CtoA

   Plasmid

   #170281

   Purpose

   Expresses yeast Tim12 (the latter with cleavable N-ter His-tag), codon-optimized for E. coli production

   Depositor

   Paul Schanda

   Article

   Weinhaupl et al Structure. 2021 May 3. pii: S0969-2126(2

   Insert

   yeast Tim12 (TIM12 Budding Yeast)
   

   Use

   Tags

   TEV-cleavable N-terminal His tag (on backbone)

   Expression

   Bacterial

   Mutation

   mutated two non-conserved Cys to Ala

   Promoter

   Available Since

   June 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1+_hsTRPA1_myc_dTom

   Plasmid

   #183179

   Purpose

   myc tagged hsTRPA1 used for cell transfection

   Depositor

   Sreekanth Chalasani

   Article

   Duque et al Nat Commun. 2022 Feb 9;13(1):600. doi: 1

   Inserts

   hsTRPA1 (TRPA1 Human)
   dTom
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAAV-CAG-eYFP

   Plasmid

   #104055

   Purpose

   An AAV genome that ubiquitously expresses the fluorescent protein eYFP from the CAG promoter

   Depositor

   Viviana Gradinaru

   Article

   Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

   Has Service

   AAV PHP.eB, AAV Retrograde, AAV2, and AAV5

   Insert

   EYFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   CAG

   Available Since

   Jan. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pECNUS4

   Plasmid

   #184887

   Purpose

   ABE cloning vector, insertion of gRNA sequence via Golden Gate with BsaI and T4 ligase, blue-white screening. Replicates in E.coli and Agrobacterium, binary vector for Agrobacterium T-DNA delivery

   Depositor

   Eunyoung Chae

   Article

   Tan et al Mol Plant Microbe Interact. 2025 Feb;38(

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   RPS5A

   Available Since

   July 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAAV-CAG-DIO-EYFP

   Plasmid

   #104052

   Purpose

   An AAV genome encoding Cre-dependent expression of the fluorescent protein EYFP from the CAG promoter

   Depositor

   Viviana Gradinaru

   Article

   Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

   Has Service

   AAV PHP.V1

   Insert

   EYFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   CAG

   Available Since

   Jan. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV-hSyn1-eYFP

   Plasmid

   #117382

   Purpose

   An AAV genome that expresses the fluorescent protein eYFP from the hSyn1 promoter

   Depositor

   Viviana Gradinaru

   Article

   Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

   Has Service

   AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

   Insert

   EYFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn1

   Available Since

   Oct. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3 Lats1 (Nigg HS189)

    Plasmid

    #41156

    Depositor

    Erich Nigg

    Article

    Chan et al Oncogene. 2005 Mar 17;24(12):2076-86.

    Insert

    Lats1 (LATS1 Human)
    

    Use

    Tags

    3xMyc and PreScission Site

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 7, 2013

    Availability

    Academic Institutions and Nonprofits only

11. pAAV-TRE-DIO-eYFP

    Plasmid

    #117383

    Purpose

    An AAV genome with tet-inducible, Cre-dependent expression of the fluorescent protein eYFP

    Depositor

    Viviana Gradinaru

    Article

    Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

    Has Service

    AAV1

    Insert

    EYFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available Since

    Oct. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pETDuet-1 TIM8,13

    Plasmid

    #170282

    Purpose

    Expresses both yeast Tim8 and yeast Tim13 (the latter with cleavable N-ter His-tag), codon-optimized for E. coli production

    Depositor

    Paul Schanda

    Article

    Sucec et al Sci Adv. 2020 Dec 18;6(51). pii: 6/51/ea

    Insert

    yeast Tim8 and Tim13 (TIM8 Budding Yeast)
    

    Use

    Tags

    TEV-cleavable N-terminal His tag on Tim13 (on bac…

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV-CAG-eYFP-3x-miR708-5p-TS

    Plasmid

    #117381

    Purpose

    An AAV genome containing miRNA target sequence (TS) 708-5p to reduce expression of the fluorescent protein eYFP in neurons

    Depositor

    Viviana Gradinaru

    Article

    Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

    Insert

    EYFP + 3 copies of miR708: CCCAGCTAGATTGTAAGCTCCTT
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Oct. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV-CAG-eYFP-3x-miR204-5p-TS

    Plasmid

    #117380

    Purpose

    An AAV genome containing miRNA target sequence (TS) 204-5p to reduce expression of the fluorescent protein eYFP in astrocytes

    Depositor

    Viviana Gradinaru

    Article

    Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

    Insert

    EYFP + 3 copies of miR204 : AGGCATAGGATGACAAAGGGAA
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Oct. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pECNUS4_MP

    Plasmid

    #228358

    Purpose

    ABE8e-SpCas9-NG Multiplex Acceptor clone for insertion of up to six gRNA cassettes via Golden Gate with BpiI and T4 ligase, blue-white screening

    Depositor

    Eunyoung Chae

    Article

    Tan et al Mol Plant Microbe Interact. 2025 Feb;38(

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    RPS5A

    Available Since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pECNUS2

    Plasmid

    #184885

    Purpose

    ABE cloning vector, insertion of gRNA sequence via Golden Gate with BsaI and T4 ligase, blue-white screening. Replicates in E.coli and Agrobacterium, binary vector for Agrobacterium T-DNA delivery

    Depositor

    Eunyoung Chae

    Article

    Tan et al Mol Plant Microbe Interact. 2025 Feb;38(

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    RPS5A

    Available Since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pECNUS3

    Plasmid

    #184886

    Purpose

    ABE cloning vector, insertion of gRNA sequence via Golden Gate with BsaI and T4 ligase, blue-white screening. Replicates in E.coli and Agrobacterium, binary vector for Agrobacterium T-DNA delivery

    Depositor

    Eunyoung Chae

    Article

    Tan et al Mol Plant Microbe Interact. 2025 Feb;38(

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    RPS5A

    Available Since

    July 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pECNUS1

    Plasmid

    #184884

    Purpose

    ABE cloning vector, insertion of gRNA sequence via Golden Gate with BsaI and T4 ligase, blue-white screening. Replicates in E.coli and Agrobacterium, binary vector for Agrobacterium T-DNA delivery

    Depositor

    Eunyoung Chae

    Article

    Tan et al Mol Plant Microbe Interact. 2025 Feb;38(

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    RPS5A

    Available Since

    July 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV_OHu19986C_myc tag

    Plasmid

    #183174

    Purpose

    used to make AAV vectors for hsTRPA1 tagged with myc

    Depositor

    Sreekanth Chalasani

    Article

    Duque et al Nat Commun. 2022 Feb 9;13(1):600. doi: 1

    Insert

    hsTRPA1 (TRPA1 Human)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSP64-mHCN2

    Plasmid

    #53060

    Purpose

    Expresses alpha subunit of mouse HCN2 channel in Xenopus oocytes

    Depositor

    Michael Sanguinetti

    Article

    Chen et al J Biol Chem. 2000 Nov 17;275(46):36465-7

    Insert

    Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2 (Hcn2 Mouse)
    

    Use

    Xenopus oocyte expression

    Tags

    Expression

    Mutation

    E55G, R237H, and K283R polymorphisms compared to …

    Promoter

    SP6

    Available Since

    Aug. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge