We narrowed to 9,424 results for: try

1. pUC19-mAirn-R-loop

   Plasmid

   #134900

   Purpose

   Generation and purification of R-loops for in vitro biochemistry

   Depositor

   Andrew Deans

   Article

   Hodson et al Cell Rep. 2022 Dec 6;41(10):111749. doi:

   Insert

   mAirn promoter (Airn Mouse)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   Feb. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC19-hSNRPN-R-loop

   Plasmid

   #134902

   Purpose

   Generation and purification of R-loops for in vitro biochemistry

   Depositor

   Andrew Deans

   Article

   Hodson et al Cell Rep. 2022 Dec 6;41(10):111749. doi:

   Insert

   hSNRPN promoter (SNRPN Human)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   Jan. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSG4K5

   Plasmid

   #74492

   Purpose

   For cloning and constitutive expression of sgRNAs in E.coli. New sgRNAs are cloned into SapI sites and multiplex sgRNAs are cloned into BsaI sites. Kanamycin resistant with pSC101 origin of rep.

   Depositor

   Xiao Wang

   Article

   Standage-Beier et al ACS Synth Biol. 2015 Nov 20;4(11):1217-2

   Insert

   sgRNA
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119

   Available since

   May 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pME-H2B-mCerulean3-P2A-mKOFP2-CAAX

   Plasmid

   #75154

   Purpose

   Nuclear mCerulean2 (blue) fluorophore and a membrane targeted KOFP2 (orange) fluorophore separated by a self-cleaving 2A peptide for use with 5’ promoters.

   Depositor

   Nicholas Cole

   Article

   Don et al Zebrafish. 2016 Sep 15.

   Insert

   H2B-mCerulean3-P2A-mKOFP2-CAAX
   

   Use

   Middle entry vector for multisite gateway three f…

   Tags

   Expression

   Mutation

   Promoter

   n/a

   Available since

   Oct. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pYPQ141-ZmUbi-RZ-As

   Plasmid

   #86196

   Purpose

   AsCpf1 gRNA entry plasmid using Zea mays Ubi promoter and ribozyme processing

   Depositor

   Yiping Qi

   Article

   Tang et al Nat Plants. 2017 Feb 17;3:17018. doi: 10

   Insert

   AsCpf1 gRNA cloning site for ribozyme cleavage
   

   Use

   CRISPR

   Tags

   Hammerhead ribozyme and HDV ribozyme

   Expression

   Plant

   Mutation

   Promoter

   Maize ubiquitin 1

   Available since

   April 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. KTK_002

   Plasmid

   #180527

   Purpose

   Entry vector containing J23104 promoter

   Depositor

   Tom Ellis

   Article

   Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

   Insert

   J23104 promoter
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR221_SLC6A3

   Plasmid

   #132160

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   Giulio Superti-Furga, RESOLUTE Consortium

   Article

   Sijben et al Sci Rep. 2021 Jan 14;11(1):1312. doi: 10

   Insert

   SLC6A3 (SLC6A3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 22, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. meffBlue chromoprotein

   Plasmid

   #117845

   Purpose

   BioBrick pSB1C3 plasmid that constitutively overexpresses meffBlue chromoprotein in E. coli

   Depositor

   Anthony Forster

   Article

   Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

   Insert

   promoter, RBS, meffBlue
   

   Use

   Synthetic Biology; Escherichia coli

   Tags

   Expression

   Mutation

   BioBrick sites removed

   Promoter

   Available since

   Aug. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDONR P4-P1R-ECFP

   Plasmid

   #48352

   Purpose

   Contributes the coding sequence for ECFP as the 5’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.

   Depositor

   Tomas Santalucia, Anna Planas

   Article

   Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:

   Insert

   ECFP
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Contains a Kozak sequence. Does not contain a sto…

   Promoter

   none

   Available since

   April 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRExT2A-CT-BmNG

    Plasmid

    #232207

    Purpose

    Endogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for C-terminal tagging: mNeongreen-3xTy-T2A-BSD-3xTy

    Depositor

    Calvin Tiengwe

    Article

    Carbajo et al Open Biol. 2025 Feb;15(2):240334. doi: 1

    Insert

    mNeongreen
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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