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I48 ELP

Plasmid

#68937

Purpose

Elastin-like polypeptide (VPGxG) with 48 repeats of Isoleucine guest residue

Depositor

Andrew MacKay

Article

Shi et al J Control Release. 2013 Nov 10;171(3):33

Insert

I48 ELP

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only

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pKAR2-Br512_Mut235

Plasmid

#179278

Purpose

Expresses Br512_Mut235 polymerase in E.coli

Depositor

Andrew Ellington

Article

Paik et al Biochemistry. 2021 Nov 11. doi: 10.1021/

Insert

Engineered Bst DNA Polymerase I Large Fragment

Use

Tags

8x His tag; Villin Headpiece 47 amino acid (HP47)…

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 16, 2022

Availability

Academic Institutions and Nonprofits only

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L1_lacZgRNA-Ck3

Plasmid

#136136

Purpose

L1 in position 3, to clone gRNA target using BbsI, lacZ blue-white screening

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

p5-MpU6:lacZgRNA

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

BsaI/ SapI domesticated

Promoter

Available since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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L1_Cas9-Ck4

Plasmid

#136135

Purpose

L1 in position 4, for AtcoCas9 (Puchta) expression in L2 CRISPR vectors

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

p5-MpEF1a:Cas9_3tNos-35S

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

BsaI/ SapI domesticated

Promoter

Available since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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L1_lacZgRNA-Ck2

Plasmid

#136137

Purpose

L1 in position 2, to clone gRNA target using BbsI, lacZ blue-white screening

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

p5-MpU6:lacZgRNA

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

BsaI/ SapI domesticated

Promoter

Available since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-PA-B/BRISBANE/60/2008

Plasmid

#187920

Purpose

Expresses PA subunit of Influenza B/Brisbane/60/2008 virus polymerase in mammalian cells

Depositor

Arindam Mondal

Article

Kedia et al Front Microbiol. 2022 May 25;13:868367.

Insert

polymerase acidic protein

Use

Tags

UNTAGGED

Expression

Mammalian

Mutation

Promoter

CMV Promoter

Available since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-PB1-B/BRISBANE/60/2008

Plasmid

#187919

Purpose

Expresses PB1 subunit of Influenza B/Brisbane/60/2008 virus polymerase in mammalian cells

Depositor

Arindam Mondal

Article

Kedia et al Front Microbiol. 2022 May 25;13:868367.

Insert

polymerase basic protein 1

Use

Tags

UNTAGGED

Expression

Mutation

Promoter

CMV Promoter

Available since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-PB2-B/BRISBANE/60/2008

Plasmid

#187917

Purpose

Expresses PB2 subunit of Influenza B/Brisbane/60/2008 virus polymerase in mammalian cells

Depositor

Arindam Mondal

Article

Kedia et al Front Microbiol. 2022 May 25;13:868367.

Insert

polymerase basic protein 2

Use

Tags

UNTAGGED

Expression

Mutation

Promoter

CMV Promoter

Available since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pCAS1yl

Plasmid

#73226

Purpose

Constitutive expression of Cas9 and sgRNA in Yarrowia lipolytica cells

Depositor

Sheng Yang

Article

Gao et al J Ind Microbiol Biotechnol. 2016 Aug;43(

Inserts

Cas9
sgRNA

Use

CRISPR; In y. lipolytica

Tags

Expression

Bacterial

Mutation

Promoter

unknown

Available since

June 16, 2016

Availability

Academic Institutions and Nonprofits only

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pET28a-GGG-ELP(120nm)-Cys

Plasmid

#91572

Purpose

Elastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]6, with N-Terminal Sortase Recognition Sequence GGG and a C-terminal Cysteine

Depositor

Hermann Gaub

Article

Ott et al ACS Nano. 2017 Jun 12. doi: 10.1021/acsn

Insert

GGG-ELP(120nm)-Cys

Use

Tags

Cysteine and Sortase-Tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-PA-3x-FLAG-B/BRISBANE/60/2008

Plasmid

#187922

Purpose

Expresses C terminal 3xFLAG tagged PA subunit of Influenza B/Brisbane/60/2008 virus polymerase in mammalian cells

Depositor

Arindam Mondal

Article

Kedia et al Front Microbiol. 2022 May 25;13:868367.

Insert

polymerase acidic protein

Use

Tags

3xFLAG

Expression

Mutation

Promoter

CMV Promoter

Available since

Sept. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a-Cys-ELP(120nm)-LPETGG

Plasmid

#90472

Purpose

Elastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]6, with N-Terminal Cysteine and C-Terminal Sortase Recognition Sequence LPETGG

Depositor

Hermann Gaub

Article

Ott et al ACS Nano. 2017 Jun 12. doi: 10.1021/acsn

Insert

Cys-ELP(120nm)-LPETGG

Use

Tags

Cysteine and Sortase-Tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pUDE1084

Plasmid

#168500

Purpose

expression of T7RNA polymerase and CRISPR endonunclease Fncas12a

Depositor

Jean-Marc Daran

Article

Randazzo et al CRISPR J. 2021 Apr 23. doi: 10.1089/cris

Inserts

Fncas12a
T7RNA polymerase

Use

CRISPR

Tags

Expression

Mutation

Promoter

TDH3 and TEF1

Available since

Dec. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-miniSOG

Plasmid

#193913

Purpose

Control plasmid that expresses non-targeted miniSOG in mammalian cells

Depositor

Xiao Wang

Article

Sessler et al Sci Adv. 2022 Dec 9;8(49):eade1136. doi:

Insert

miniSOG

Use

AAV

Tags

Flag tag

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-miniSOG-T2A-dTomato

Plasmid

#193796

Purpose

Expresses miniSOG in the cytosol of mammalian cells, joined by a T2A cleavage site with dTomato

Depositor

Xiao Wang

Article

Sessler et al Sci Adv. 2022 Dec 9;8(49):eade1136. doi:

Insert

miniSOG

Use

AAV

Tags

Flag tag

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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MCV-Rep-

Plasmid

#32058

Depositor

Patrick Moore

Article

Feng et al PLoS One. 2011;6(7):e22468. Epub 2011 Ju

Insert

MCV-Rep- genome

Use

Tags

Expression

Mutation

Single cytosine to adenine (C/A) mutation at 2,07…

Promoter

none from vector. Insert contains native promoter…

Available since

Oct. 25, 2011

Availability

Academic Institutions and Nonprofits only

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RNAPII_CTD27-52_K5S

Plasmid

#98679

Purpose

Expresses 6xHis-tagged C-terminal domain of RNA polymerase II with K5S

Depositor

Nicolas Fawzi

Article

Janke et al Biochemistry. 2017 Oct 11. doi: 10.1021/

Insert

RNA polymerase II C-terminal domain (POLR2A Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

K1838S, K1859S, K1866S, K1873S, K1887S

Promoter

Available since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pWzyE-His

Plasmid

#196669

Purpose

Expression of C-terminally His10-tagged WzyE from Pectobacterium atrosepticum in E.coli

Depositor

James Naismith

Article

Weckener et al Open Biol. 2023 Mar;13(3):220373. doi: 1

Insert

WzyE

Use

Tags

TEV cleavable His10

Expression

Bacterial

Mutation

Promoter

araBAD

Available since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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pUC57-fsr1

Plasmid

#179866

Purpose

Encodes the polyketide synthase Fsr1 from Fusarium vanettenii codon optimized for expression in Saccharomyces cerevisiae

Depositor

Jens Laurids Sørensen

Article

Bejenari et al J Microbiol Biol Educ. 2023 Nov 27;24(3)

Insert

Fusarubin polyketide synthase

Use

Synthetic Biology

Tags

Expression

Mutation

Codon optimized for expression in Saccharomyces c…

Promoter

Available since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-miniSOG-C-Far

Plasmid

#193914

Purpose

Control plasmid that expresses miniSOG targeted to the cell membrane in mammalian cells

Depositor

Xiao Wang

Article

Sessler et al Sci Adv. 2022 Dec 9;8(49):eade1136. doi:

Insert

miniSOG

Use

AAV

Tags

Farnesylation signal sequence and Flag tag

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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