We narrowed to 9,340 results for: UTY

1. pJH750

   Plasmid

   #172170

   Purpose

   Microscopy inclusion body assay

   Depositor

   Matthew Bennett

   Article

   Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

   Insert

   pCDF.3f2, deleted sumo
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Evolved PylBCD operon; SUMO tag deleted from PylB…

   Promoter

   Available since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pJH751

   Plasmid

   #172171

   Purpose

   Microscopy inclusion body assay

   Depositor

   Matthew Bennett

   Article

   Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

   Insert

   pCDF.JM10.1, deleted sumo
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Evolved PylBCD operon; SUMO tag deleted from PylB…

   Promoter

   Available since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pJH768v2

   Plasmid

   #172173

   Purpose

   PylB overexpression and purification

   Depositor

   Matthew Bennett

   Article

   Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

   Insert

   PT7/Lac-[His6-TEV-SUMO-PylB.3f2]
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   pET28a_LacI_PT7/Lac-[His6-TEV-SUMO-PylB(N61S, E84…

   Promoter

   Available since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pJH769v2

   Plasmid

   #172174

   Purpose

   PylB overexpression and purification

   Depositor

   Matthew Bennett

   Article

   Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

   Insert

   PT7/Lac-[His6-TEV-SUMO-PylB.JM10.1]
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   pET28a_LacI_PT7/Lac-[His6-TEV-SUMO-PylB(N61S, E84…

   Promoter

   Available since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pJH191

   Plasmid

   #172149

   Purpose

   Accessory Plasmid for PACE of pylBCD variants

   Depositor

   Matthew Bennett

   Article

   Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

   Insert

   Ppsp gIII.2TAG, luxAB; Plpp [eG SD8] 32A-Nter; PproK pylT
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   gIII(P29*, Y184*); [strong promoter eG, strong RB…

   Promoter

   Available since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pJH61

   Plasmid

   #172143

   Purpose

   Accessory Plasmid for PACE of pylBCD variants

   Depositor

   Matthew Bennett

   Article

   Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

   Insert

   Ppsp gIII.1TAG, luxAB; Plpp [eG SD8] pylS; PproK pylT
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   gIII(P29*); [strong promoter eG, strong RBS SD8] …

   Promoter

   Available since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pJH190

   Plasmid

   #172148

   Purpose

   Accessory Plasmid for PACE of pylBCD variants

   Depositor

   Matthew Bennett

   Article

   Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

   Insert

   Ppsp gIII.1TAG, luxAB; Plpp [eG SD8] 32A-Nter; PproK pylT
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   gIII(P29*); [strong promoter eG, strong RBS SD8] …

   Promoter

   Available since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. GST-OPTNS177DS473D

   Plasmid

   #171425

   Purpose

   For the purifcation of phosphorylation mimic protein

   Depositor

   James Hurley

   Article

   Chang et al Sci Adv. 2021 Apr 23;7(17). pii: 7/17/ea

   Insert

   OPTN (OPTN Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   phosphorylation mutant (S177D, S473D)

   Promoter

   Available since

   July 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. GST-TEVcs- FIP200 delta641-779-MBP

   Plasmid

   #171411

   Purpose

   For the purifcation of human ULK1 truncated complex

   Depositor

   James Hurley

   Article

   Chang et al Sci Adv. 2021 Apr 23;7(17). pii: 7/17/ea

   Insert

   FIP200 (RB1CC1 Synthetic)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   truncation (delta641-779-MBP)

   Promoter

   Available since

   July 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pJH198_pE12_hyCal2-eGFP

    Plasmid

    #160411

    Purpose

    Gateway entry clone containing postsynaptic HyCal component (HyNaC2) for HySyn system fused to eGFP

    Depositor

    Daniel Colón-Ramos

    Article

    Hawk et al Nat Commun. 2021 Aug 9;12(1):4795. doi:

    Insert

    pDONR221 HyCal2::eGFP
    

    Use

    Other

    Tags

    eGFP

    Expression

    Mutation

    Promoter

    Available since

    May 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pJH200_pE12_hyCal7-eGFP

    Plasmid

    #160413

    Purpose

    Gateway entry clone containing postsynaptic HyCal component (HyNaC7) for HySyn system fused to eGFP

    Depositor

    Daniel Colón-Ramos

    Article

    Hawk et al Nat Commun. 2021 Aug 9;12(1):4795. doi:

    Insert

    pDONR221 HyCal7::eGFP
    

    Use

    Other

    Tags

    eGFP

    Expression

    Mutation

    Promoter

    Available since

    May 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pJH201_pE12_hyCal7-mCherry

    Plasmid

    #160414

    Purpose

    Gateway entry clone containing postsynaptic HyCal component (HyNaC7) for HySyn system fused to mCherry

    Depositor

    Daniel Colón-Ramos

    Article

    Hawk et al Nat Commun. 2021 Aug 9;12(1):4795. doi:

    Insert

    pDONR221 HyCal7::mCherry
    

    Use

    Other

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    Available since

    May 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJH202_pE12_hyCal9-eGFP

    Plasmid

    #160415

    Purpose

    Gateway entry clone containing postsynaptic HyCal component (HyNaC9) for HySyn system fused to eGFP

    Depositor

    Daniel Colón-Ramos

    Article

    Hawk et al Nat Commun. 2021 Aug 9;12(1):4795. doi:

    Insert

    pDONR221 HyCal9::eGFP
    

    Use

    Other

    Tags

    eGFP

    Expression

    Mutation

    Promoter

    Available since

    May 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pJH203_pE12_hyCal9-mCherry

    Plasmid

    #160416

    Purpose

    Gateway entry clone containing postsynaptic HyCal component (HyNaC9) for HySyn system fused to mCherry

    Depositor

    Daniel Colón-Ramos

    Article

    Hawk et al Nat Commun. 2021 Aug 9;12(1):4795. doi:

    Insert

    pDONR221 HyCal9::mCherry
    

    Use

    Other

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    Available since

    May 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. AP-VAMP7(low)

    Plasmid

    #167998

    Purpose

    positive selection PACE Accessory Plasmid

    Depositor

    David Liu

    Article

    Blum et al Science. 2021 Feb 19;371(6531):803-810.

    Insert

    VAMP7(K121-R180)-T7PAP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET-CV-VAMP1

    Plasmid

    #168022

    Purpose

    Expresses eCFP-VAMP1(T29-K89)-mVenus in bacterial cells

    Depositor

    David Liu

    Article

    Blum et al Science. 2021 Feb 19;371(6531):803-810.

    Insert

    eCFP-VAMP1(T29-K89)-mVenus
    

    Use

    Tags

    Hisx6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET-CV-VAMP4

    Plasmid

    #168025

    Purpose

    Expresses eCFP-VAMP4(P48-R107)-mVenus in bacterial cells

    Depositor

    David Liu

    Article

    Blum et al Science. 2021 Feb 19;371(6531):803-810.

    Insert

    eCFP-VAMP4(P48-R107)-mVenus
    

    Use

    Tags

    Hisx6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET-CV-VAMP3

    Plasmid

    #168024

    Purpose

    Expresses eCFP-VAMP3(S11-R69)-mVenus in bacterial cells

    Depositor

    David Liu

    Article

    Blum et al Science. 2021 Feb 19;371(6531):803-810.

    Insert

    eCFP-VAMP3(S11-R69)-mVenus
    

    Use

    Tags

    Hisx6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET-CV-VAMP7

    Plasmid

    #168027

    Purpose

    Expresses eCFP-VAMP7(K121-R180)-mVenus in bacterial cells

    Depositor

    David Liu

    Article

    Blum et al Science. 2021 Feb 19;371(6531):803-810.

    Insert

    eCFP-VAMP7(K121-R180)-mVenus
    

    Use

    Tags

    Hisx6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET-CV-VAMP5

    Plasmid

    #168026

    Purpose

    Expresses eCFP-VAMP5(M1-Q60)-mVenus in bacterial cells

    Depositor

    David Liu

    Article

    Blum et al Science. 2021 Feb 19;371(6531):803-810.

    Insert

    eCFP-VAMP5(M1-Q60)-mVenus
    

    Use

    Tags

    Hisx6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. VanR - F165V

    Plasmid

    #158965

    Purpose

    Wild type VanR where the residue in position 165 was mutated from Phenylalanine to Valine. This mutation abolished the response to vanillic acid. The gene is under the control of the TEF1 promoter.

    Depositor

    Michael Jensen

    Article

    et al Biotechnology Notes, Volume 1, 2020, Pag

    Insert

    VanR - F165V
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Wild type VanR from Caulobacter crescentus with m…

    Promoter

    TEF1

    Available since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. VanR - F165R

    Plasmid

    #158966

    Purpose

    Wild type VanR where the residue in position 165 was mutated from Phenylalanine to Arginine. This mutation abolished the response to vanillic acid. The gene is under the control of the TEF1 promoter.

    Depositor

    Michael Jensen

    Article

    et al Biotechnology Notes, Volume 1, 2020, Pag

    Insert

    VanR - F165R
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Wild type VanR from Caulobacter crescentus with m…

    Promoter

    TEF1

    Available since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pL2-1 ConjEpi PtEF2bsd Pt49202HMGRVenusThrFLAG

    Plasmid

    #139963

    Purpose

    Over-expression of native 3-hydroxy-3-methyl glutaryl CoA reductase

    Depositor

    Raffaela Abbriano

    Article

    Jaramillo-Madrid et al Pharmaceuticals (Basel). 2020 Dec 21;13(

    Insert

    ConjEpi PtEF2_bsd_PttEF2 Pt49202_HMGRVenusThrFLAG_Ptt49202
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pMIII-Neo-AM

    Plasmid

    #153427

    Purpose

    Retroviral (MSCV) expression of ametrine fluorescent protein

    Depositor

    Maki Nakayama

    Article

    Mann et al Front Immunol. 2020 Apr 9;11:633. doi: 1

    Insert

    ametrine
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pMIII-Neo-LO

    Plasmid

    #153428

    Purpose

    Retroviral (MSCV) expression of LSSmOrange fluorescent protein

    Depositor

    Maki Nakayama

    Article

    Mann et al Front Immunol. 2020 Apr 9;11:633. doi: 1

    Insert

    LSSmOrange
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pMP89b CvTA V124N

    Plasmid

    #141207

    Purpose

    Chromobacterium violaceum transaminase with enhances affinity for PLP. TEV cleaving site after the N-terminal Histag.

    Depositor

    Francesca Paradisi

    Article

    Roura Padrosa et al Front Bioeng Biotechnol. 2019 Oct 18;7:2

    Insert

    CvTA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    V124N

    Promoter

    Available since

    June 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pEM03

    Plasmid

    #135417

    Purpose

    Expression of Hygromycin resistance from Spizellomyces Hsp70 promoter

    Depositor

    Nicolas Buchler

    Article

    Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

    Insert

    SpunHsp70pr
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Hsp70pr

    Available since

    May 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pHCKan-yibDp-cimA3.7

    Plasmid

    #134595

    Purpose

    Low Phosphate inducible mutant citramalate synthase

    Depositor

    Michael Lynch

    Article

    Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

    Insert

    cimA3.7
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    I47V, E114V, H126Q, T204A, L238S

    Promoter

    yibDp

    Available since

    May 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pHCKan-yibDp-SBS-mCherry

    Plasmid

    #134598

    Purpose

    Low Phosphate inducible SBS (bonryl-diphosphate) synthase with C-terminal mCherry)

    Depositor

    Michael Lynch

    Article

    Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

    Insert

    SBS-mCherry
    

    Use

    Tags

    mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    yibDp

    Available since

    May 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pHCKan-T7-CBD-hGLY

    Plasmid

    #134940

    Purpose

    T7 based expression of human glycine acyltransferase with an N-terminal chitin binding domain

    Depositor

    Michael Lynch

    Article

    Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

    Insert

    human glycine acyltransferase 2 (GLYATL2 )
    

    Use

    Tags

    chitin binding domain

    Expression

    Bacterial

    Mutation

    Promoter

    t7

    Available since

    May 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pTKhc-yibDp-GFP-β20cp6

    Plasmid

    #127060

    Purpose

    Low phosphate inducible circular permutation of GFP, which is a substrate of the lon protease

    Depositor

    Michael Lynch

    Article

    Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

    Insert

    GFPcp6-beta20
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    yibD

    Available since

    May 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. p3E-rab26-CA

    Plasmid

    #109746

    Purpose

    Multisite gateway vector for 3' tagging with constitutively active rab26

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    rab26 (rab26 Zebrafish)
    

    Use

    Zebrafish plasmids

    Tags

    Expression

    Mutation

    constitutively active

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. p3E-rab38a-CA

    Plasmid

    #109748

    Purpose

    Multisite gateway vector for 3' tagging with constitutively active rab38a

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    rab38a (rab38a Zebrafish)
    

    Use

    Zebrafish plasmids

    Tags

    Expression

    Mutation

    constitutively active

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. p3E-rab6a-CA

    Plasmid

    #109733

    Purpose

    Multisite gateway vector for 3' tagging with constitutively active rab6a

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    rab6a (rab6a Zebrafish)
    

    Use

    Zebrafish plasmids

    Tags

    Expression

    Mutation

    constitutively active

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. p3E-rab4a-CA

    Plasmid

    #109736

    Purpose

    Multisite gateway vector for 3' tagging with constitutively active rab4a

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    rab4a (rab4a Zebrafish)
    

    Use

    Zebrafish plasmids

    Tags

    Expression

    Mutation

    constitutively active

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pQlinkH-YbATP-grasp

    Plasmid

    #129639

    Purpose

    Expression plasmid of ATP-grasp from Y. bercovieri

    Depositor

    Matthias Bochtler

    Article

    Piasecka et al J Mol Biol. 2018 Mar 2;430(5):611-627. d

    Insert

    YbATP-grasp
    

    Use

    Tags

    His6-tag

    Expression

    Bacterial

    Mutation

    Promoter

    pQE

    Available since

    Nov. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pBT271.361

    Plasmid

    #127846

    Purpose

    clone A3A-W98Y CBEs by SapI Golden Gate

    Depositor

    David Liu

    Article

    Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

    Insert

    A3A-W98Y
    

    Use

    Tags

    Expression

    Mutation

    W98Y

    Promoter

    Available since

    July 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. FLAG-MBP1-E322TAG-E309C-W340A-CAAX.pcDNA3-k

    Plasmid

    #127409

    Purpose

    Expresses MBP in mammalian cells. Amber codon at AA# 322 as a noncanonical amino acid incorporation site

    Depositor

    Sharona Gordon

    Article

    Gordon et al Elife. 2018 Jun 20;7. pii: 37248. doi: 1

    Insert

    Maltose Binding Protein
    

    Use

    Tags

    CAAX and FLAG

    Expression

    Mammalian

    Mutation

    Amino acid mutations: E322 to Amber codon, E309C …

    Promoter

    CMV

    Available since

    July 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. FLAG-MBP1-K295TAG-W340A-CAAX.pcDNA3-k

    Plasmid

    #127399

    Purpose

    Expresses MBP in mammalian cells. Amber codon at AA# 295 as a noncanonical amino acid incorporation site

    Depositor

    Sharona Gordon

    Article

    Gordon et al Elife. 2018 Jun 20;7. pii: 37248. doi: 1

    Insert

    Maltose Binding Protein
    

    Use

    Tags

    CAAX and FLAG

    Expression

    Mammalian

    Mutation

    Amino acid mutations: K295 to Amber codon and W34…

    Promoter

    CMV

    Available since

    July 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. FLAG-MBP1-E322TAG-E309C-W340A.pcDNA3-k

    Plasmid

    #127408

    Purpose

    Expresses MBP in mammalian cells. Amber codon at AA# 322 as a noncanonical amino acid incorporation site

    Depositor

    Sharona Gordon

    Article

    Gordon et al Elife. 2018 Jun 20;7. pii: 37248. doi: 1

    Insert

    Maltose Binding Protein
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Amino acid mutations: E322 to Amber codon, E309C …

    Promoter

    CMV

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. FLAG-MBP1-E322TAG-K305H-E309H-W340A.pcDNA3-k

    Plasmid

    #127406

    Purpose

    Expresses MBP in mammalian cells. Amber codon at AA# 322 as a noncanonical amino acid incorporation site

    Depositor

    Sharona Gordon

    Article

    Gordon et al Elife. 2018 Jun 20;7. pii: 37248. doi: 1

    Insert

    Maltose Binding Protein
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Amino acid mutations: E322 to Amber codon, K305H,…

    Promoter

    CMV

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. FLAG-MBP1-E322TAG-W340A-CAAX.pcDNA3-k

    Plasmid

    #127405

    Purpose

    Expresses MBP in mammalian cells. Amber codon at AA# 322 as a noncanonical amino acid incorporation site

    Depositor

    Sharona Gordon

    Article

    Gordon et al Elife. 2018 Jun 20;7. pii: 37248. doi: 1

    Insert

    Maltose Binding Protein
    

    Use

    Tags

    CAAX and FLAG

    Expression

    Mammalian

    Mutation

    Amino acid mutations: E322 to Amber codon and W34…

    Promoter

    CMV

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. FLAG-MBP1-K295TAG-W340A-S233H-T237C.pcDNA3-k

    Plasmid

    #127402

    Purpose

    Expresses MBP in mammalian cells. Amber codon at AA# 295 as a noncanonical amino acid incorporation site

    Depositor

    Sharona Gordon

    Article

    Gordon et al Elife. 2018 Jun 20;7. pii: 37248. doi: 1

    Insert

    Maltose Binding Protein
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Amino acid mutations: K295 to Amber codon, W340A,…

    Promoter

    CMV

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. FLAG-MBP1-K295TAG-W340A-S233H-T237H.pcDNA3-k

    Plasmid

    #127400

    Purpose

    Expresses MBP in mammalian cells. Amber codon at AA# 295 as a noncanonical amino acid incorporation site

    Depositor

    Sharona Gordon

    Article

    Gordon et al Elife. 2018 Jun 20;7. pii: 37248. doi: 1

    Insert

    Maltose Binding Protein
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Amino acid mutations: K295 to Amber codon, W340A,…

    Promoter

    CMV

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pMX-eSOX2AHK

    Plasmid

    #118826

    Purpose

    Retroviral expression of a mutant of Sox2 that improves efficiency of iPSC generation

    Depositor

    Ralf Jauch

    Article

    Veerapandian et al Stem Cell Reports. 2018 Aug 14;11(2):593

    Insert

    eSox2AHK (Sox2 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    AHK: E46A, I53H

    Promoter

    Available since

    April 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. kank1a_pJet1.2

    Plasmid

    #121978

    Purpose

    for generating kank1a riboprobes for WISH in zebrafish

    Depositor

    GuangJun Zhang

    Article

    Hensley et al Sci Rep. 2016 Jun 13;6:27816. doi: 10.10

    Insert

    kank1a (si:dkey-146l2.1 Zebrafish)
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Feb. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. mdm1_pJet1.2

    Plasmid

    #121977

    Purpose

    for generating mdm1 riboprobes for WISH in zebrafish

    Depositor

    GuangJun Zhang

    Article

    Hensley et al PLoS One. 2016 Sep 22;11(9):e0163229. do

    Insert

    mdm1 (mdm1 Zebrafish)
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Feb. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pET28a-YJR129c

    Plasmid

    #85118

    Purpose

    bacterial expression of YJR129c

    Depositor

    Pal Falnes

    Article

    Davydova et al J Biol Chem. 2014 Oct 31;289(44):30499-5

    Insert

    EFM3 (EFM3 Budding Yeast)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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49. pET15b+R2(L171C)

    Plasmid

    #108239

    Purpose

    Expresses PdR-PCNA2(L171C) fusion protein in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

    Insert

    PdR-PCNA2(L171C) fusion protein
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    PCNA2,L171C

    Promoter

    T7 promoter

    Available since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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50. pET15T+1X

    Plasmid

    #108188

    Purpose

    PCNA1-PdX fusion protein

    Depositor

    Teruyuki Nagamune

    Article

    Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

    Insert

    PCNA1-PdX fusion protein
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    C73S/C85S (PdX)

    Promoter

    T7 promoter

    Available since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge