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We narrowed to 749 results for: UTY

Showing: 1 - 20 of 749 results

  1. Distribution to Industry

    Type
    Collection
    ... Distribution to Industry Featured Collections Search Plasmids...Purification Optimization Kit Using SUMO Protease Elution Protein Characterization Christopher Bahl A validation...agreement between the recipient and the depositing institution(s). It governs the transfer of biological materials...distribute plasmids on behalf of the depositing institutions. These agreements indicate the language which...which we are allowed to use for distribution. Please see the Sample Agreements on our Technology Transfer... by an authorized signatory at the recipient institution. An authorized signatory is someone who can execute...execute legal documents on behalf of the recipient institution. Once the MTA has been signed, it can be submitted...

  2. Evolution of Lab Techniques

    Type
    Blog Post
    ...equivalents and see what we can learn from their evolution. Southern blotting and quantitative real-time... less expensive sequencing fuels a sequencing revolution that still prevails. Sequencing whole genomes... evolution...

  4. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    ...examples that have guided your resolution processes in the past. Types of Resolution There are of course many...can do it. If Party X proposes solution A, and Party Y proposes solution B, with proper guidance X and ...to create solution C. And if a conflict resolver does their job well, not only will solution C be better...realized I might have some minor skills in conflict resolution after college, when I discovered that many of...any of their disagreements. In fact, conflict resolution isn’t just about resolving conflicts between ...meant to be a comprehensive guide to Conflict Resolution. There are countless classes and resources that...Conflict Before explaining my approach to conflict resolution, it’s important to recognize that there are different...

  5. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    ...insertion site.   The Sleeping Beauty transposon system The Sleeping Beauty (SB) transposon system is one...gene-of-interest, either constitutively or regulated by doxycycline. The Sleeping Beauty transposase is proving... Mates L, et al. Molecular evolution of a novel hyperactive Sleeping Beauty transposase enables robust...can translocate within the genome. The Sleeping Beauty transposase gains its name from the fact that it...changes. The basic functional version of the Sleeping Beauty transposon (SB10), developed in 1997, continues...efficiencies of integrating viral vectors. New Sleeping Beauty transposase vectors Even more recently, Eric Kowarz...transposase system - one set of vectors for robust constitutive expression (pSBbi derivatives) and one set of...

  6. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type
    Blog Post
    ...with some solutions based on what I’ve learned. I hope you can apply some of these solutions to your own...Reproducibility. The minisymposium focused on solutions to reproducibility issues in the biological sciences...of reproducibility issues and their potential solutions. Prior to this event, I gave my own talk on reproducibility...understanding how the body works, generating new therapeutics, and sometimes formulating new biological concepts...ultimately wasting both time and money. The obvious solution to this problem would be for traditional journals...issues are complicated, we have many potential solutions that could save both your lab and the entire research...

  7. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    ...quickly following the tried and true process of evolution. Watch this video showing bacteria evolve antibiotic...resistance genes survive and multiply. Factors contributing to the development of resistance include (Figure...before this information becomes available. For routine infections, like strep throat, testing for antibiotic... however, is fight smarter, not harder. We can utilize tactics like rotating antibiotic treatments, so...

  8. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    ...and highlights some of the latest breakthrough therapeutic approaches. What is Rett Syndrome? Caused by ...dysfunction, distinctive hand movements, and show autistic-like behaviors.  The role of MECP2 in Rett Syndrome...physiological levels. Much research is now focused on therapeutic strategies that use gene replacement therapy,...combined technical expertise to develop a viable therapeutic, the researchers developed adeno-associated viral... repair the mutation (Le et al., 2019). Beam Therapeutics, co-founded by Harvard Investigators and Addgene... Rett syndrome program being pursued at Vico Therapeutics, co-founded by Gail Mandel. There are numerous...

  9. Antibodies 101: Antibody Engineering and Directed Evolution

    Type
    Blog Post
    ...benefits antibody-based therapeutics. Whether antibodies are used directly as therapeutic agents or serve as...BioRender.com.   Antibody directed evolution Directed evolution is a method employed to rapidly change...Structure-guided directed evolution If you desire a bit more control, structure guided directed evolution might be more...antibody engineering, to improve reagents and therapeutics. What is antibody engineering (and why do we...haven’t discussed in depth is antibody directed evolution. This is a larger process of gradually changing...gene shuffling, and structure-guided directed evolution. The nice thing about these methods is that they...how they are screened. When conducting antibody evolution experiments, you’ll typically go through this ...

  10. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    ...field, let's look at how prokaryotes originally utilized CRISPR/Cas as a powerful and adaptive defense ...prokaryotes developed an adaptive immune system that utilized various cas genes not only to store a record of...proteins and PAM sequences. A total of eight evolutionarily conserved CRISPR subtypes comprised of more..., 83% of archaeal and 45% of bacterial genomes utilize this system (4). Makarova et al. grouped the systems...subtypes using polythetic classification based on evolutionary similarity (5). Type II CRISPR/Cas is the system...against invading phages. Rising to the challenge - Evolution of anti-CRISPR genes in phages The CRISPR/Cas ...

  11. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    ...developed today. In this post, I will highlight the evolution of the  Brainbow multicolor labeling system - ...- a perfect example of the continued utility of Cre-lox. Check out our previous blog post, Plasmids 101...highlights the trend in biology towards single-cell resolution imaging. Sanes and Lichtman endeavored to create... of stained sections, were limited due to low resolution or were very time-intensive, necessitating the...

  13. Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

    Type
    Blog Post
    ....   Sleeping Beauty, a transposon system from ancient fish Surprisingly, Sleeping Beauty is not only a... “awakened from a long evolutionary sleep,” it was aptly named Sleeping Beauty (Ivics et al., 1997). We...rooms such as Groucho, Sonic Hedgehog, Sleeping Beauty, Cookie Monster, Spaghetti Squash, Tinman, and ...but also a biological transposon system. Sleeping Beauty is a synthetic transposon system that can be used... transposon system? Scientists can use Sleeping Beauty transposons as a non-viral system to integrate ...

  14. Isolating a Monoclonal Cell Population by Limiting Dilution

    Type
    Protocol
    ... a 1:100 dilution of the homogenized cell solution by adding 10 µL homogenized cell solution to 990 µL...Cloning by Limiting Dilution Isolating a Monoclonal Cell Population by Limiting Dilution You may also like...cell solution later in this protocol. Isolating a Monoclonal Cell Population by Limiting Dilution Isolate...homogenized cell solution is transferred. Transfer cells from the homogenized cell solution into the conditioned...cell solution at a concentration of 5 cells/mL. Prepare approximately 10 mL of this cell solution for ...medium, prepare this cell solution in fresh complete medium. This 5 cells/mL solution will be used to seed ... quantitate the homogenized cell solution. Homogenized cell solution concentration: 4 × 10 5 cells/mL ...

  15. Biosensor AAV Preps

    Type
    Collection
    ...iAChSnFR none Constitutive 1 Looger 137955 pAAV.CAG.iAChSnFR CAG iAChSnFR none Constitutive 1, 9 Looger ... none Constitutive 1, 5, rg* Looger 176753 AAV-mDlx-jGCaMP8f-WPRE Dlx jGCaMP8f none Constitutive 1, 9,...jGCaMP8f none Constitutive 1, 9 Looger 179254 AAV-CAG-jGCaMP8f-WPRE CAG jGCaMP8f none Constitutive 1, 9, rg...ribo-jGCaMP8s none Constitutive 1 Fyhn 169256 AAV-hSyn-Soma-jGCaMP8s Syn soma-jGCaMP8s none Constitutive 1, 9, rg...none Constitutive 1, 5, 9, rg* Looger 176755 AAV-mDlx-jGCaMP8s-WPRE Dlx jGCaMP8s none Constitutive 1, 9...jGCaMP8s none Constitutive 1, 9 Looger 179256 AAV-CAG-jGCaMP8s-WPRE CAG jGCaMP8s none Constitutive 1, 9, rg...ribo-jGCaMP8m none Constitutive 1 Fyhn 169257 AAV-hSyn-Soma-jGCaMP8m Syn soma-jGCaMP8m none Constitutive 1, 9, rg...

  16. Control AAV Preps

    Type
    Collection
    ...gfaABC1D tdTomato Constitutive 5 Baljit Khakh 50465 pAAV-hSyn-EGFP hSyn EGFP Constitutive 1, 2, 5, 8, 9, ...tdTomato Constitutive 5 Hongkui Zeng 58909 pAAV-GFAP104-mCherry GFAP104 mCherry Constitutive 5 Edward ...PI.eGFP.WPRE.bGH GFA104 EGFP Constitutive 5 Philip Haydon 104055 pAAV-CAG-eYFP CAG EYFP Constitutive 2, 5, rg*, PHP....-GFP Constitutive PHPeB Viviana Gradinaru 105530 pAAV.CMV.PI.EGFP.WPRE.bGH CMV EGFP Constitutive 1, 2,...ffLuciferase Constitutive 8 James M. Wilson 105534 pAAV.TBG.PI.LacZ.bGH TBG LacZ Constitutive 8 James M....TBG none Constitutive 8 James M. Wilson 105539 pAAV.hSyn.eGFP.WPRE.bGH Syn EGFP Constitutive 1 James M...CamKII EGFP Constitutive 1, 5 James M. Wilson 105535 pAAV.TBG.PI.eGFP.WPRE.bGH TBG EGFP Constitutive 8 James...

  17. Optogenetics AAV Preps

    Type
    Collection
    ...mScarlet Constitutive 9 Christopher Harvey 59170 pAAV-Syn-Chronos-GFP Syn Chronos GFP Constitutive 1, 5, ...ChETA EYFP Constitutive 9 Karl Deisseroth 135633 pAAV-S5E2-C1V1-eYFP E2 C1V1 EYFP Constitutive 1, 5, 9, ...mCherry Constitutive 8 Roger Tsien 99039 pAAV-CamKII-ArchT-GFP (PV2527) CaMKII ArchT GFP Constitutive 1, 5...EYFP Constitutive 2, 5 Karl Deisseroth 137151 pAAV-nEF-NpHR3.3-EYFP nEF NpHR 3.3 EYFP Constitutive 8 Karl...dependent Flp dependent Cre and Flp dependent Constitutive Serotype AAV1 AAV2 AAV5 AAV8 AAV9 AAVrg PHPeB...PI 20071 pACAGW-ChR2-Venus-AAV CAG ChR2 Venus Constitutive 1, 9 Karel Svoboda 83898 pAAV-mDlx-ChR2-mCherry-Fishell...mCherry-Fishell-3 Dlx ChR2 mCherry Constitutive 1, 9, rg* Gordon Fishell 135634 pAAV-S5E2-ChR2-mCherry nEF...

  18. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ... or mammalian expression (Constitutive or Cre-dependent) Directed Evolution of a Selective and Sensitive...and Archon 2 (Constitutive or Cre-dependent) A robotic multidimensional directed evolution approach applied...Fast Genetically Encoded Calcium Indicators (Constitutive or Cre-dependent) jGCaMP8 Fast Genetically Encoded...High-performance GFP-based calcium indicators (Constitutive or Cre-dependent) High-performance calcium sensors...calcium sensors for imaging neural activity (Constitutive or Cre-dependent) Ultrasensitive fluorescent...mRuby2 for imaging neural activity (GCaMP6f/m/s) (Constitutive or Cre-dependent) Cell-specific restoration ...Imaging intraorganellar Ca(2+) at subcellular resolution using CEPIA. Nat Commun. 2014 Jun 13;5:4153. ...

  19. Genetic Code Expansion

    Type
    Collection
    ...options, such a 4-base pair codons, have also been utilized. A tRNA that recognizes this codon. An aminoacyl-tRNA... can be mutated and screened through directed evolution to charge the tRNA with a different amino acid...and evolve proteins for various purposes from therapeutics to biopolymers. Tips for Success Before beginning... IBBN (G2) synthetase M. jannaschii 4-(2′-bromoisobutyramido)-phenylalanine (IBBN) and structurally analogous...Howard Hang 126035 pEVOL-ABK PylRS M. barkeri 3’-azibutyl-N-carbamoyl-lysine (AbK) Bacterial TAG Andrea ...Expresses yeast TrpRS/tRNA pair instead. For directed evolution of the liberated EcTrpRS/tRNA pair to incorporate...

  20. AAV Molecular Tools

    Type
    Collection
    ...Activity Serotype PI 99118 pAAV-CAG-tTA CAG-driven, constitutive Expression of the tet-off transactivator (tTA...34910 paavCAG-pre-mGRASP-mCerulean CAG-driven, constitutive Expression of pre-synaptic mGRASP-mCerulean ...34912 paavCAG-post-mGRASP-2A-dTomato CAG-driven, constitutive Expression of post-synaptic mGRASP-dTomato for...AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA CMV-driven, constitutive Expression of human codon-optimized Cas9 from...Serotype PI 149415 pAAV-TBG-ER-TurboID TBG-driven, constitutive Expression of ER-localized TurboID (with HA ...Serotype PI 58376 pAAV/D377Y-mPCSK9 hAAT-driven, constitutive Expression of mutant (D377Y) murine PCSK9 for...Serotype PI 232351 pAAV-ATLASsnCre Syn-driven, constitutive Anterograde transsynaptic tracer protein to ...
Showing: 1 - 20 of 749 results