We narrowed to 2,695 results for: GUS

1. pACYC_HA-ZmSAE1_ZmSAE2(TRUNC)-FLAG

   Plasmid

   #91945

   Purpose

   Bacterial expression of maize SAE1 and truncated SAE2

   Depositor

   Richard Vierstra

   Article

   Augustine et al Plant Physiol. 2016 Jul;171(3):2191-210.

   Inserts

   HA-ZmSAE1
   ZmSAE2-FLAG
   

   Tags

   FLAG and HA

   Expression

   Bacterial

   Mutation

   Truncation of ZmSAE2 to mimic a splice variant

   Available Since

   June 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCnRed

   Plasmid

   #232908

   Purpose

   Electroporation vector for expression of the lambda Red recombineering system in Cupriavidus necator.

   Depositor

   Anita Emmerstorfer-Augustin

   Article

   Arhar et al ACS Synth Biol. 2025 Feb 24. doi: 10.102

   Insert

   gam (gam Synthetic, phage Lambda)
   

   Use

   Synthetic Biology

   Promoter

   Pbad-rha

   Available Since

   June 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPB-EF1A-Puro-hTBK1-EGFP

   Plasmid

   #221553

   Purpose

   Expresses GFP-tagged human TBK1 in mammalian cells. Compatible with piggybac transposase for genome integration.

   Depositor

   Shawn Ferguson

   Article

   Talaia et al bioRxiv [Preprint]. 2023 Dec 17:2023.12.

   Insert

   TBK1 (TBK1 Human)
   

   Use

   Piggybac

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCnCre

   Plasmid

   #232907

   Purpose

   Electroporation vector for expression of Cre recombinase in Cuprividus necator

   Depositor

   Anita Emmerstorfer-Augustin

   Article

   Arhar et al ACS Synth Biol. 2025 Feb 24. doi: 10.102

   Insert

   Cre recombinase (cre Synthetic)
   

   Use

   Cre/Lox and Synthetic Biology

   Promoter

   Ptac-cymO

   Available Since

   June 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHInt-AppA-KanR-loxP

   Plasmid

   #232910

   Purpose

   Plasmid for marker free integration of E. coli appA at the phac1 locus of Cupriavidus necator.

   Depositor

   Anita Emmerstorfer-Augustin

   Article

   Arhar et al ACS Synth Biol. 2025 Feb 24. doi: 10.102

   Insert

   AppA (appA )
   

   Use

   Synthetic Biology

   Promoter

   Pj5

   Available Since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKESaPar

   Plasmid

   #211829

   Purpose

   Low copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein and a RP4 partitioning system to increase segregational stability.

   Depositor

   Anita Emmerstorfer-Augustin

   Article

   Arhar et al Microb Cell Fact. 2024 Jan 3;23(1):9. do

   Insert

   eGFP
   

   Expression

   Bacterial

   Promoter

   T5

   Available Since

   Feb. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKERepPar

   Plasmid

   #211827

   Purpose

   Medium copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein and a RP4 partitioning system to increase segregational stability.

   Depositor

   Anita Emmerstorfer-Augustin

   Article

   Arhar et al Microb Cell Fact. 2024 Jan 3;23(1):9. do

   Insert

   eGFP
   

   Expression

   Bacterial

   Promoter

   T5

   Available Since

   Feb. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pQCXIH-PSM-Rb

   Plasmid

   #37106

   Depositor

   Joseph Nevins

   Article

   Angus et al Oncogene. 2012 Jan 16. doi: 10.1038/onc.

   Insert

   Rb (RB1 Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   aa 379-928 only; large pocket of human Rb with aa…

   Promoter

   CMV

   Available Since

   Sept. 21, 2012

   Availability

   Academic Institutions and Nonprofits only

9. RhGC(BE)_pGEM

   Plasmid

   #85469

   Purpose

   The rhodopsin-guanylyl cyclase of the aquatic fungus Blastocladiella emersonii enables fast optical control of cGMP signaling

   Depositor

   Peter Hegemann

   Article

   Scheib et al Sci Signal. 2015 Aug 11;8(389):rs8. doi:

   Insert

   rhodopsin-guanylyl cyclase
   

   Expression

   Mammalian

   Promoter

   T7 Promoter

   Available Since

   Jan. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. ALFY-FYVE 1G

    Plasmid

    #197045

    Purpose

    Expression plasmid for the FYVE domain from ALFY for recombinant expression in E. coli.

    Depositor

    Michael Ragusa

    Article

    Reinhart et al Traffic. 2021 Jan;22(1-2):23-37. doi: 10

    Insert

    ALFY (WDFY3 Human)
    

    Tags

    6xHis-GST-TEV

    Expression

    Bacterial

    Available Since

    Dec. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. px459-RhebL1 sgRNA

    Plasmid

    #133769

    Purpose

    Expresses Cas9 and human RhebL1 sgRNA

    Depositor

    Shawn Ferguson

    Article

    Angarola et al Mol Biol Cell. 2019 Oct 15;30(22):2750-2

    Insert

    RhebL1 sgRNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Nov. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPpT4- HTA1prom-TIR1 F74G -FLAG

    Plasmid

    #189728

    Purpose

    Enables expression of OsTIR1(F74G) from the HTA1 promoter, used to implement the AID2 system in Komagataella phaffii (original name pLB220)

    Depositor

    Anita Emmerstorfer-Augustin

    Article

    Lehmayer et al iScience. 2022 Aug 6;25(9):104888. doi:

    Insert

    OsTIR1
    

    Tags

    FLAG

    Expression

    Yeast

    Promoter

    HTA1

    Available Since

    Sept. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. px459-Rheb sgRNA

    Plasmid

    #133768

    Purpose

    Expresses Cas9 and human Rheb sgRNA

    Depositor

    Shawn Ferguson

    Article

    Angarola et al Mol Biol Cell. 2019 Oct 15;30(22):2750-2

    Insert

    Rheb sgRNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAdTrackCMV-PSM-Rb

    Plasmid

    #37107

    Depositor

    Joseph Nevins

    Article

    Angus et al Oncogene. 2012 Jan 16. doi: 10.1038/onc.

    Insert

    Rb (RB1 Human)
    

    Use

    Adenoviral

    Expression

    Mammalian

    Mutation

    aa 379-928 only; large pocket of human Rb with aa…

    Promoter

    CMV

    Available Since

    May 31, 2012

    Availability

    Academic Institutions and Nonprofits only

15. pRSF_6His-ZmSUMO1a-GG_ZmSCE1d(C94S)-Myc

    Plasmid

    #91935

    Purpose

    Bacterial expression of maize SUMO1a and inactive SCE1d

    Depositor

    Richard Vierstra

    Article

    Augustine et al Plant Physiol. 2016 Jul;171(3):2191-210.

    Inserts

    6His-ZmSUMO1a-GG
    ZmSCE1d(C94S)-Myc
    

    Tags

    6His and Myc

    Expression

    Bacterial

    Mutation

    C94S active site mutation and Truncation of ZmSUM…

    Available Since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRSF_6His-ZmSUMO1a-GG_ZmSCE1b(C94S)-Myc

    Plasmid

    #91933

    Purpose

    Bacterial expression of maize SUMO1a and inactive SCE1b

    Depositor

    Richard Vierstra

    Article

    Augustine et al Plant Physiol. 2016 Jul;171(3):2191-210.

    Inserts

    6His-ZmSUMO1a-GG
    ZmSCE1b(C94S)-Myc
    

    Tags

    6His and Myc

    Expression

    Bacterial

    Mutation

    C94S active site mutation and Truncation of ZmSUM…

    Available Since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. SuperClomeleon

    Plasmid

    #203724

    Purpose

    An improved engineered fluorescent ratiometric optogenetic sensor to detect inhibitory synaptic activity via changes in intracellular chloride

    Depositor

    George Augustine

    Article

    Grimley et al J Neurosci. 2013 Oct 9;33(41):16297-309.

    Insert

    Cerulean linked topaz fluorescent protein
    

    Expression

    Bacterial and Mammalian

    Mutation

    Cerulean, modified Topaz Fluorescent Protein with…

    Available Since

    Feb. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSpCas9-2A-Puro-TBK1-gRNA (PX459)

    Plasmid

    #221550

    Purpose

    Expresses Cas9 and gRNA for disruption of TBK1 gene in human cells

    Depositor

    Shawn Ferguson

    Article

    Talaia et al bioRxiv [Preprint]. 2023 Dec 17:2023.12.

    Insert

    gRNA targeting human TBK1 (TBK1 Human)
    

    Expression

    Mammalian

    Promoter

    u6

    Available Since

    July 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPB-EF1A-Puro-hTBK1-E696K-EGFP

    Plasmid

    #221554

    Purpose

    Expresses GFP-tagged human TBK1 with E696K mutation in mammalian cells. Compatible with piggybac transposase for genome integration.

    Depositor

    Shawn Ferguson

    Article

    Talaia et al bioRxiv [Preprint]. 2023 Dec 17:2023.12.

    Insert

    TBK1 (TBK1 Human)
    

    Use

    Piggybac

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Changed glutamate 696 to lysine

    Promoter

    EF1a

    Available Since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCDNA3.1(+) miniTurboID-6xGGSG linker-FKBPF36V-2xHA

    Plasmid

    #200641

    Purpose

    Expresses miniTurboID-FKBPF36V-2xHA in mammalian cells.

    Depositor

    Justin English, Fleur Ferguson

    Article

    Tao et al Nat Commun. 2023 Dec 4;14(1):8016. doi:

    Insert

    miniTurboID-6xGGSG linker-FKBPF36V-2xHA
    

    Tags

    HA-tag

    Expression

    Mammalian

    Promoter

    T7

    Available Since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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