We narrowed to 2,759 results for: GUS

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pRSF_6His-ZmSUMO1a-GG_ZmSCE1b-Myc

Plasmid

#91932

Purpose

Bacterial expression of maize SUMO1a and SCE1b

Depositor

Richard Vierstra

Article

Augustine et al Plant Physiol. 2016 Jul;171(3):2191-210.

Inserts

6His-ZmSUMO1a-GG
ZmSCE1b-Myc

Tags

6His and Myc

Expression

Bacterial

Mutation

Truncation of ZmSUMO1a after the diGlycine motif

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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pRSF_6His-ZmSUMO1a-GG_ZmSCE1d-Myc

Plasmid

#91934

Purpose

Bacterial expression of maize SUMO1a and SCE1d

Depositor

Richard Vierstra

Article

Augustine et al Plant Physiol. 2016 Jul;171(3):2191-210.

Inserts

6His-ZmSUMO1a-GG
ZmSCE1d-Myc

Tags

6His and Myc

Expression

Bacterial

Mutation

Truncation of ZmSUMO1a after the diGlycine motif

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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pPpT4- TEF2prom-TIR1F74G-FLAG

Plasmid

#189727

Purpose

Enables expression of OsTIR1(F74G) from the TEF2 promoter, used to implement the AID2 system in Komagataella phaffii (original name pLB219)

Depositor

Anita Emmerstorfer-Augustin

Article

Lehmayer et al iScience. 2022 Aug 6;25(9):104888. doi:

Insert

OsTIR1

Tags

FLAG

Expression

Yeast

Promoter

TEF2

Available Since

Sept. 20, 2022

Availability

Academic Institutions and Nonprofits only

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Atg11 [699-800] pHis2

Plasmid

#190264

Purpose

Codon optimized Atg11 amino acids 699-800 from S. cerevisiae for expression in E. coli.

Depositor

Michael Ragusa

Article

Margolis et al J Mol Biol. 2020 Oct 2;432(21):5752-5764

Insert

ATG11

Tags

6xHis-TEV

Expression

Bacterial

Promoter

T7

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pPpT4- HTA1prom-TIR1-FLAG

Plasmid

#189725

Purpose

Enables expression of OsTIR1 from the HTA1 promoter, used to implement the AID system in Komagataella phaffii (original name pLLpLL001)

Depositor

Anita Emmerstorfer-Augustin

Article

Lehmayer et al iScience. 2022 Aug 6;25(9):104888. doi:

Insert

OsTIR1

Tags

FLAG

Expression

Yeast

Promoter

HTA1

Available Since

Sept. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pPpT4- TEF2prom-TIR1-FLAG

Plasmid

#189726

Purpose

Enables expression of OsTIR1 from the TEF2 promoter, used to implement the AID system in Komagataella phaffii (original name pLL002)

Depositor

Anita Emmerstorfer-Augustin

Article

Lehmayer et al iScience. 2022 Aug 6;25(9):104888. doi:

Insert

OsTIR1

Tags

FLAG

Expression

Yeast

Promoter

TEF2

Available Since

Sept. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pACYC_HA-ZmSAE1_ZmSAE2(TRUNC)-FLAG

Plasmid

#91945

Purpose

Bacterial expression of maize SAE1 and truncated SAE2

Depositor

Richard Vierstra

Article

Augustine et al Plant Physiol. 2016 Jul;171(3):2191-210.

Inserts

HA-ZmSAE1
ZmSAE2-FLAG

Tags

FLAG and HA

Expression

Bacterial

Mutation

Truncation of ZmSAE2 to mimic a splice variant

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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pCnCre

Plasmid

#232907

Purpose

Electroporation vector for expression of Cre recombinase in Cuprividus necator

Depositor

Anita Emmerstorfer-Augustin

Article

Arhar et al ACS Synth Biol. 2025 Feb 24. doi: 10.102

Insert

Cre recombinase (cre Synthetic)

Use

Cre/Lox and Synthetic Biology

Promoter

Ptac-cymO

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pCnRed

Plasmid

#232908

Purpose

Electroporation vector for expression of the lambda Red recombineering system in Cupriavidus necator.

Depositor

Anita Emmerstorfer-Augustin

Article

Arhar et al ACS Synth Biol. 2025 Feb 24. doi: 10.102

Insert

gam (gam phage Lambda, Synthetic)

Use

Synthetic Biology

Promoter

Pbad-rha

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pHInt-AppA-KanR-loxP

Plasmid

#232910

Purpose

Plasmid for marker free integration of E. coli appA at the phac1 locus of Cupriavidus necator.

Depositor

Anita Emmerstorfer-Augustin

Article

Arhar et al ACS Synth Biol. 2025 Feb 24. doi: 10.102

Insert

AppA (appA )

Use

Synthetic Biology

Promoter

Pj5

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pKESaPar

Plasmid

#211829

Purpose

Low copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein and a RP4 partitioning system to increase segregational stability.

Depositor

Anita Emmerstorfer-Augustin

Article

Arhar et al Microb Cell Fact. 2024 Jan 3;23(1):9. do

Insert

eGFP

Expression

Bacterial

Promoter

T5

Available Since

Feb. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pKERepPar

Plasmid

#211827

Purpose

Medium copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein and a RP4 partitioning system to increase segregational stability.

Depositor

Anita Emmerstorfer-Augustin

Article

Arhar et al Microb Cell Fact. 2024 Jan 3;23(1):9. do

Insert

eGFP

Expression

Bacterial

Promoter

T5

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pQCXIH-PSM-Rb

Plasmid

#37106

Depositor

Joseph Nevins

Article

Angus et al Oncogene. 2012 Jan 16. doi: 10.1038/onc.

Insert

Rb (RB1 Human)

Use

Retroviral

Expression

Mammalian

Mutation

aa 379-928 only; large pocket of human Rb with aa…

Promoter

CMV

Available Since

Sept. 21, 2012

Availability

Academic Institutions and Nonprofits only
RhGC(BE)_pGEM

Plasmid

#85469

Purpose

The rhodopsin-guanylyl cyclase of the aquatic fungus Blastocladiella emersonii enables fast optical control of cGMP signaling

Depositor

Peter Hegemann

Article

Scheib et al Sci Signal. 2015 Aug 11;8(389):rs8. doi:

Insert

rhodopsin-guanylyl cyclase

Expression

Mammalian

Promoter

T7 Promoter

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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ALFY-FYVE 1G

Plasmid

#197045

Purpose

Expression plasmid for the FYVE domain from ALFY for recombinant expression in E. coli.

Depositor

Michael Ragusa

Article

Reinhart et al Traffic. 2021 Jan;22(1-2):23-37. doi: 10

Insert

ALFY (WDFY3 Human)

Tags

6xHis-GST-TEV

Expression

Bacterial

Available Since

Dec. 7, 2023

Availability

Academic Institutions and Nonprofits only

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px459-RhebL1 sgRNA

Plasmid

#133769

Purpose

Expresses Cas9 and human RhebL1 sgRNA

Depositor

Shawn Ferguson

Article

Angarola et al Mol Biol Cell. 2019 Oct 15;30(22):2750-2

Insert

RhebL1 sgRNA

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pPpT4- HTA1prom-TIR1 F74G -FLAG

Plasmid

#189728

Purpose

Enables expression of OsTIR1(F74G) from the HTA1 promoter, used to implement the AID2 system in Komagataella phaffii (original name pLB220)

Depositor

Anita Emmerstorfer-Augustin

Article

Lehmayer et al iScience. 2022 Aug 6;25(9):104888. doi:

Insert

OsTIR1

Tags

FLAG

Expression

Yeast

Promoter

HTA1

Available Since

Sept. 20, 2022

Availability

Academic Institutions and Nonprofits only

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px459-Rheb sgRNA

Plasmid

#133768

Purpose

Expresses Cas9 and human Rheb sgRNA

Depositor

Shawn Ferguson

Article

Angarola et al Mol Biol Cell. 2019 Oct 15;30(22):2750-2

Insert

Rheb sgRNA

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pAdTrackCMV-PSM-Rb

Plasmid

#37107

Depositor

Joseph Nevins

Article

Angus et al Oncogene. 2012 Jan 16. doi: 10.1038/onc.

Insert

Rb (RB1 Human)

Use

Adenoviral

Expression

Mammalian

Mutation

aa 379-928 only; large pocket of human Rb with aa…

Promoter

CMV

Available Since

May 31, 2012

Availability

Academic Institutions and Nonprofits only
pRSF_6His-ZmSUMO1a-GG_ZmSCE1d(C94S)-Myc

Plasmid

#91935

Purpose

Bacterial expression of maize SUMO1a and inactive SCE1d

Depositor

Richard Vierstra

Article

Augustine et al Plant Physiol. 2016 Jul;171(3):2191-210.

Inserts

6His-ZmSUMO1a-GG
ZmSCE1d(C94S)-Myc

Tags

6His and Myc

Expression

Bacterial

Mutation

C94S active site mutation and Truncation of ZmSUM…

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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