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pCASP-SptP167-3G124-HilA

Plasmid

#153322

Purpose

Triggers secretion of SptP167 fused to the 3G124nc anti-GFP DARPin from Salmonella upon induction with arabinose

Depositor

Josef Penninger

Article

Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

Insert

SptP167-3G124 DARPin

Use

Salmonella expression

Tags

Expression

Mutation

Promoter

Available since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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pCASP-SptP120-3m160-HilA

Plasmid

#153325

Purpose

Triggers secretion of SptP120 fused to the 3m160 anti-mCherry DARPin from Salmonella upon induction with arabinose

Depositor

Josef Penninger

Article

Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

Insert

SptP120-3m160 DARPin

Use

Salmonella expression

Tags

Expression

Mutation

Promoter

Available since

July 16, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-HA-mIFITM1-C49,50,83,103A

Plasmid

#58421

Purpose

Expresses murine IFITM1-C49,50,83,103A with an N-terminal HA tag in mammalian cells (cysteine-less mutant that is not palmitoylated).

Depositor

Jacob Yount

Article

Hach et al J Virol. 2013 Sep;87(17):9923-7. doi: 10

Insert

IFITM1 (Ifitm1 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Cysteines 49, 50, 83, and 103 to Alanine

Promoter

CMV IE

Available since

Sept. 9, 2014

Availability

Academic Institutions and Nonprofits only
pCASP-SptP120-E3_5-HilA

Plasmid

#153326

Purpose

Triggers secretion of SptP120 fused to the E3_5 DARPin from Salmonella upon induction with arabinose

Depositor

Josef Penninger

Article

Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

Insert

SptP120-E3_5 DARPin

Use

Salmonella expression

Tags

Expression

Mutation

Promoter

Available since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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pCASP-SptP120-K27-HilA

Plasmid

#153328

Purpose

Triggers secretion of SptP120 fused to the K27 DARPin from Salmonella upon induction with arabinose

Depositor

Josef Penninger

Article

Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

Insert

SptP120-K27 DARPin

Use

Salmonella expression

Tags

Expression

Mutation

Promoter

Available since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
DCT.VV.Orf221

Plasmid

#12909

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf221

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 29, 2006

Availability

Academic Institutions and Nonprofits only
miR-144 Trap

Plasmid

#60934

Purpose

to knockdown endogenous miR144 levels; lentiviral vector containing 8x copies of complementary miR-144-binding sites cloned 3' to GFP under the control of a biPGK promoter

Depositor

Curt Civin

Article

Kim et al Br J Haematol. 2014 Oct 14. doi: 10.1111

Insert

miR-144 Trap

Use

Lentiviral

Tags

GFP

Expression

Mutation

Promoter

bidirectional phosphoglycerate kinase promoter (b…

Available since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only
myc-hIFITM3-F63Q

Plasmid

#104363

Purpose

Expresses human IFITM3-F63Q with an N-terminal myc tag in mammalian cells.

Depositor

Jacob Yount

Article

Chesarino et al EMBO Rep. 2017 Oct;18(10):1740-1751. doi

Insert

IFITM3 (IFITM3 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

mutated the following amino acid: F63Q

Promoter

CMV

Available since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pVRc11_987

Plasmid

#49733

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

AS11_987

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pLux

Available since

Jan. 6, 2014

Availability

Academic Institutions and Nonprofits only

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8xCTS1_BpiI-cloning-site_SV40polyA

Plasmid

#124547

Purpose

Destination cloning vector for tRNA-flanked sgRNA. A BpiI placeholder is present between a minimal ADE promoter and SV40 polyA sequence.

Depositor

Tudor Fulga

Article

Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

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pCASP-SptP120-NS1-HilA

Plasmid

#153327

Purpose

Triggers secretion of SptP120 fused to the NS1 monobody from Salmonella upon induction with arabinose

Depositor

Josef Penninger

Article

Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

Insert

SptP120-NS1 Monobody

Use

Salmonella expression

Tags

Expression

Mutation

Promoter

Available since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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RAB14 3'UTR D1D2

Plasmid

#61492

Purpose

luciferase reporter containing RAB14 3' UTR with both predicted MiR144 binding site deleted

Depositor

Curt Civin

Article

Kim et al Br J Haematol. 2014 Oct 14. doi: 10.1111

Insert

RAB14 3' UTR (RAB14 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

contains position 837-1637 (numbering relative to…

Promoter

PGK

Available since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only
pCMV-myc-mIFITM3

Plasmid

#58458

Purpose

Expresses murine IFITM3 with an N-terminal myc tag in mammalian cells.

Depositor

Jacob Yount

Article

Chesarino et al J Biol Chem. 2014 Apr 25;289(17):11986-9

Insert

IFITM3 (Ifitm3 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV IE

Available since

Sept. 16, 2014

Availability

Academic Institutions and Nonprofits only
pVRc15_436

Plasmid

#49735

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

AS15_436

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pLux

Available since

Jan. 6, 2014

Availability

Academic Institutions and Nonprofits only

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p2Flag CMV2-ZO-2-1st-PDZ-mut

Plasmid

#27416

Depositor

Marius Sudol

Article

Oka et al Biochem J. 2010 Nov 25. 432(3):461-72.

Insert

Zonula Occludens 2 (TJP2 Human)

Use

Tags

2xFlag

Expression

Mammalian

Mutation

K38E, F44H, I46E and V48E

Promoter

Available since

April 12, 2011

Availability

Academic Institutions and Nonprofits only
DCT.VV.Orf175

Plasmid

#12863

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf175

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2006

Availability

Academic Institutions and Nonprofits only
DCT.VV.Orf256

Plasmid

#12944

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf256

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 29, 2006

Availability

Academic Institutions and Nonprofits only
DCT.VV.Orf234

Plasmid

#12922

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf234

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 29, 2006

Availability

Academic Institutions and Nonprofits only
DCT.VV.Orf226

Plasmid

#12914

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf226

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 29, 2006

Availability

Academic Institutions and Nonprofits only
RAB14 3'UTR D1

Plasmid

#61490

Purpose

luciferase reporter containing RAB14 3' UTR with the first predicted MiR144 binding site deleted

Depositor

Curt Civin

Article

Kim et al Br J Haematol. 2014 Oct 14. doi: 10.1111

Insert

RAB14 3' UTR (RAB14 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

contains position 837-1637 (numbering relative to…

Promoter

PGK

Available since

Jan. 9, 2015

Availability

Academic Institutions and Nonprofits only