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pA_higher-CheY-pMag-mNeonGreen

Plasmid

#253260

Purpose

Encodes the fusion of CheY, pMag, and mNeonGreen driven with the proA promoter and wild type T7 RBS

Depositor

Aravinthan DT Samuel

Article

Vrabioiu et al Proc Natl Acad Sci U S A. 2026 Mar 10;12

Insert

CheY_pMag_mNeonGreen

Tags

pMag and mNeonGreen

Expression

Bacterial

Available Since

March 19, 2026

Availability

Academic Institutions and Nonprofits only

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pXL_dSSN1_EF1a-tNGFR-2A-Puro

Plasmid

#200553

Purpose

Dual barcoding cassettes for SSN-seq; Barcode Set 1

Depositor

Pawel Mazur

Article

Lu et al Nat Biomed Eng. 2023 Aug 31. doi: 10.103

Inserts

SSN.guide
SSN.F30
tNGFR

Use

Lentiviral

Promoter

EF1a, Human U6, and Mouse U6

Available Since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pXL_dSSN2_EF1a-tNGFR-2A-Puro

Plasmid

#201112

Purpose

Dual barcoding cassettes for SSN-seq; Barcode Set 2

Depositor

Pawel Mazur

Article

Lu et al Nat Biomed Eng. 2023 Aug 31. doi: 10.103

Inserts

SSN.guide
SSN.F30
tNGFR

Use

Lentiviral

Promoter

EF1a, Human U6, and Mouse U6

Available Since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pXL_dSSN3_EF1a-tNGFR-2A-Puro

Plasmid

#201113

Purpose

Dual barcoding cassettes for SSN-seq; Barcode Set 3

Depositor

Pawel Mazur

Article

Lu et al Nat Biomed Eng. 2023 Aug 31. doi: 10.103

Inserts

SSN.guide
SSN.F30
tNGFR

Use

Lentiviral

Promoter

EF1a, Human U6, and Mouse U6

Available Since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pXL_dSSN4_EF1a-tNGFR-2A-Puro

Plasmid

#201114

Purpose

Dual barcoding cassettes for SSN-seq; Barcode Set 4

Depositor

Pawel Mazur

Article

Lu et al Nat Biomed Eng. 2023 Aug 31. doi: 10.103

Inserts

SSN.guide
SSN.F30
tNGFR

Use

Lentiviral

Promoter

EF1a, Human U6, and Mouse U6

Available Since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pXL_dSSN5_EF1a-tNGFR-2A-Puro

Plasmid

#201115

Purpose

Dual barcoding cassettes for SSN-seq; Barcode Set 5

Depositor

Pawel Mazur

Article

Lu et al Nat Biomed Eng. 2023 Aug 31. doi: 10.103

Inserts

SSN.guide
SSN.F30
tNGFR

Use

Lentiviral

Promoter

EF1a, Human U6, and Mouse U6

Available Since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pXL_dSSN6_EF1a-tNGFR-2A-Puro

Plasmid

#201116

Purpose

Dual barcoding cassettes for SSN-seq; Barcode Set 6

Depositor

Pawel Mazur

Article

Lu et al Nat Biomed Eng. 2023 Aug 31. doi: 10.103

Inserts

SSN.guide
SSN.F30
tNGFR

Use

Lentiviral

Promoter

EF1a, Human U6, and Mouse U6

Available Since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pXL_dSSN7_EF1a-tNGFR-2A-Puro

Plasmid

#201117

Purpose

Dual barcoding cassettes for SSN-seq; Barcode Set 7

Depositor

Pawel Mazur

Article

Lu et al Nat Biomed Eng. 2023 Aug 31. doi: 10.103

Inserts

SSN.guide
SSN.F30
tNGFR

Use

Lentiviral

Promoter

EF1a, Human U6, and Mouse U6

Available Since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pXL_dSSN8_EF1a-tNGFR-2A-Puro

Plasmid

#201118

Purpose

Dual barcoding cassettes for SSN-seq; Barcode Set 8

Depositor

Pawel Mazur

Article

Lu et al Nat Biomed Eng. 2023 Aug 31. doi: 10.103

Inserts

SSN.guide
SSN.F30
tNGFR

Use

Lentiviral

Promoter

EF1a, Human U6, and Mouse U6

Available Since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

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hSyn-Cre-hSyn-PreRNA barcode landing pad (PPIB)

Plasmid

#247718

Purpose

The set of RNA constructs used to produce barcode library in the optimized connectome-seq experiments. Also used for Basescope experiment.

Depositor

Boxuan Zhao

Article

Chen et al Nat Methods. 2026 Mar 12. doi: 10.1038/s

Insert

Cre and PreRNA

Use

AAV

Available Since

Dec. 10, 2025

Availability

Academic Institutions and Nonprofits only

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hSyn-Cre-hSyn-PostRNA barcode landing pad (dapB)

Plasmid

#247720

Purpose

The set of RNA constructs used to produce barcode library in the optimized connectome-seq experiments. Also used for Basescope experiment.

Depositor

Boxuan Zhao

Article

Chen et al Nat Methods. 2026 Mar 12. doi: 10.1038/s

Insert

Cre and PostRNA

Use

AAV

Available Since

Dec. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pA_higher-Opto_N449S

Plasmid

#253258

Purpose

Opto-CheY N449S expressed using the proA promoter and wild type T7 RBS

Depositor

Aravinthan DT Samuel

Article

Vrabioiu et al Proc Natl Acad Sci U S A. 2026 Mar 10;12

Insert

Opto-CheY with N449S mutation

Tags

wild type T7 RBS

Expression

Bacterial

Available Since

March 19, 2026

Availability

Academic Institutions and Nonprofits only

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pA-Opto

Plasmid

#253255

Purpose

Opto-CheY expressed using the proA promoter and T7 RBS variant GAAGGAGgT

Depositor

Aravinthan DT Samuel

Article

Vrabioiu et al Proc Natl Acad Sci U S A. 2026 Mar 10;12

Insert

Opto-CheY

Expression

Bacterial

Mutation

T7 RBS variant GAAGGAGgT

Available Since

April 6, 2026

Availability

Academic Institutions and Nonprofits only

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p5g-Opto_N449S

Plasmid

#253285

Purpose

Opto-CheY N449S expressed using the pro5 promoter and T7 RBS variant GAAGGAGgT

Depositor

Aravinthan DT Samuel

Article

Vrabioiu et al Proc Natl Acad Sci U S A. 2026 Mar 10;12

Insert

Opto-CheY with N449S mutation

Tags

T7 RBS variant GAAGGAGgT

Expression

Bacterial

Available Since

April 6, 2026

Availability

Academic Institutions and Nonprofits only

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pA-Opto_N449S

Plasmid

#253256

Purpose

Opto-CheY N449S expressed using the proA promoter and T7 RBS variant GAAGGAGgT

Depositor

Aravinthan DT Samuel

Article

Vrabioiu et al Proc Natl Acad Sci U S A. 2026 Mar 10;12

Insert

Opto-CheY with N449S mutation

Tags

T7 RBS variant GAAGGAGgT

Expression

Bacterial

Available Since

March 19, 2026

Availability

Academic Institutions and Nonprofits only

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pA-Opto_V416T

Plasmid

#253257

Purpose

Opto-CheY V416T expressed using the proA promoter and T7 RBS variant GAAGGAGgT

Depositor

Aravinthan DT Samuel

Article

Vrabioiu et al Proc Natl Acad Sci U S A. 2026 Mar 10;12

Insert

Opto-CheY with V416T mutation

Expression

Bacterial

Mutation

T7 RBS variant GAAGGAGgT

Available Since

March 19, 2026

Availability

Academic Institutions and Nonprofits only

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pVenusCfC

Plasmid

#128567

Purpose

Protein interaction screening tool with Gateway-compatible BiFC vectors comprising of a set of 4 vectors permiting N or C fusions of candidate genes to the N or C fragment of Split-Venus.

Depositor

Oliver Vugrek

Article

Lepur et al J Biomol Screen. 2016 Dec;21(10):1100-11

Insert

Venus

Expression

Mammalian

Available Since

Jan. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pVenusCfN

Plasmid

#128566

Purpose

Protein interaction screening tool with Gateway-compatible BiFC vectors comprising of a set of 4 vectors permiting N or C fusions of candidate genes to the N or C fragment of Split-Venus.

Depositor

Oliver Vugrek

Article

Lepur et al J Biomol Screen. 2016 Dec;21(10):1100-11

Insert

Venus

Expression

Mammalian

Available Since

Jan. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2-HA-GAAZFP-FokI-RR

Plasmid

#18754

Depositor

Scot Wolfe

Article

Meng et al Nat Biotechnol. 2008 Jun . 26(6):695-701

Insert

zinc finger nuclease

Expression

Bacterial

Mutation

RNA expression vector for expressing the zinc fin…

Available Since

July 11, 2008

Availability

Academic Institutions and Nonprofits only

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pCS2-Flag-TTGZFP-FokI-DD

Plasmid

#18755

Depositor

Scot Wolfe

Article

Meng et al Nat Biotechnol. 2008 Jun . 26(6):695-701

Insert

zinc finger nuclease

Expression

Bacterial

Mutation

RNA expression vector for expressing the zinc fin…

Available Since

July 11, 2008

Availability

Academic Institutions and Nonprofits only

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