We narrowed to 6,833 results for: Proc
-
Plasmid#219820PurposehU6-driven pre-gRNA plasmid for CasRx applications with puromycin resistance. 5' processed DR followed by BbsI sites for guide cloning (Adapted from plasmid #10954)DepositorTypeEmpty backboneUseCRISPRExpressionMammalianPromoterhU6Available SinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only
-
pOpen-phi29pol
Plasmid#165573PurposeDNA polymerase responsible for protein-primed viral DNA replication by strand displacement with high processivity and fidelity. Possesses three enzymatic activities: DNA synthesis (polymerase), primer terminal protein (TP) deoxynucleotidylation, and 3' to 5' exonuclease activity.DepositorInsertphi29 DNA Polymerase
UseSynthetic BiologyAvailable SinceAug. 24, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pXR003_puroR
Plasmid#219819PurposehU6-driven sgRNA plasmid for CasRx applications with puromycin resistance. 5' processed DR followed by BbsI sites for guide cloning (Adapted from plasmid #109053)DepositorTypeEmpty backboneUseCRISPRExpressionMammalianPromoterhU6Available SinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
psubCAG-WPRE-hVEGF-D-Strept
Plasmid#119227PurposePlasmid encodes Strep-tagged full-length (unprocessed) VEGF-D of human origin to be used for stimulation of lymphangiogenesis in mice in vivo.DepositorAvailable SinceDec. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pANY4-pL18-ccdB
Plasmid#164898PurposeTwo valuable constructs for either IPTG-induced or optimized temperature-induced expression can be simultaneously obtained in a single cloning process by utilizing plasmid pANY4-pL18-ccdB.DepositorInsertccdB cassette
TagsHis-tagExpressionBacterialAvailable SinceApril 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHH0103 HYPC WW domain #2
Plasmid#104232PurposeBacterial expression of WW domain #2 from HYPCDepositorInsertHYPC WW domain #2 (PRPF40B Human)
TagsGSTExpressionBacterialMutationcodon optimized for expression in bacteriaPromotertacAvailable SinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
3'dmDR-CMV-CasRx-bGHpolyA
Plasmid#229556PurposeExpresses CasRx (RfxCas13d) with double processed direct repeats (15nt) and a non-target spacer in between at 3' endDepositorInsertCasRx
UseCRISPRExpressionMammalianAvailable SinceFeb. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
1203U-U6-gRNA-pCMV-wtCas9-NG-P2A-mcherry-3'dmDR
Plasmid#221448PurposeExpresses wtCas9-NG with double processed direct repeats (15nt) and a non-target spacer in between at 3' end, mCherry tag, and the gRNA targeting HEK293T-site3DepositorInsertswtCas9-NG
mCherry
UseCRISPRExpressionMammalianAvailable SinceFeb. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pANY4-pR18/pL18-ccdB
Plasmid#164899PurposeTwo valuable constructs for either IPTG-induced or optimized temperature-induced expression can be simultaneously obtained in a single cloning process by utilizing plasmid pANY4-pR18/pL18-ccdB.DepositorInsertccdB cassette
TagsHis-tagExpressionBacterialAvailable SinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOpen-T4 g32
Plasmid#165560PurposeA single-stranded DNA binding protein required for bacteriophage T4 replication and repair; Used extensively to stabilize and mark regions of ssDNA for electron microscopic examination of intracellular DNA structure, to increase yield and processivity of reverse transcriptase during RT-PCR, and to increase yield and specificity of PCR products from soil samples.DepositorInsertT4 gene 32
UseSynthetic BiologyAvailable SinceAug. 16, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits