We narrowed to 3,143 results for: BFP

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pU6-sgRNA EF1Alpha-puro-T2A-BFP.ERH2

Plasmid

#164801

Purpose

For CRISPRi knockdown of ERH by lentiviral delivery of ERH gRNA2

Depositor

David Bartel

Article

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Inserts

ERH CRISPRi gRNA2
puro-T2A-BFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1Alpha and U6

Available Since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV-mtagBFP-2A STING V155M 342STOP

Plasmid

#102594

Purpose

Lentivector to express STING V155M delta 342 and mTagBFP

Depositor

Nicolas Manel

Article

Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

Insert

STING (STING1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

V155M delta 342

Promoter

SFFV

Available Since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pCCL-PGK-SPdCas9-BFP-DNMT3B(E697A)

Plasmid

#71214

Purpose

dCas9 fused to BFP and the human DNMT3B catalytically inactive domain

Depositor

Yonglun Luo

Article

Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

Insert

dSpCas9-BFP-DNMT3B(E697A), DNMT3B catalytic domain with inactivation mutation E697A

Tags

dSpCas9-BFP-DNMT3B(E697A)

Expression

Mammalian

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pHBS1504 [IBB-GFP-mCherry3E]-[BFP-TDP43 F-S]

Plasmid

#133328

Purpose

To test the effect of sequence on TDP43 splicing activity

Depositor

Rajat Rohatgi

Article

Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

Insert

TARDBP mutant (TARDBP Human)

Expression

Mammalian

Mutation

All F to S in CTD of full-length TDP43

Available Since

Nov. 6, 2019

Availability

Academic Institutions and Nonprofits only

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RAB11A-BFP HDRT Source (pTR 145)

Plasmid

#112014

Purpose

DNA sequence source for amplifying an HDR template to tag endogenous human RAB11A gene with BFP

Depositor

Alexander Marson

Article

Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

RAB11A-BFP HDRT (RAB11A Human)

Use

CRISPR and Synthetic Biology

Available Since

March 13, 2019

Availability

Academic Institutions and Nonprofits only

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pCAGGS-mTagBFP2-T2A-sfGFP-iCre-ERT2

Plasmid

#128166

Purpose

Monomeric TagBFP2 and sfGFP-iCre-ERT2 recombinase (PAPGSTM N-terminus, unskippable linker)

Depositor

Mario Capecchi

Article

Nam et al Neural Dev. 2020 Mar 17;15(1):3. doi: 10

Insert

mTagBFP2-T2A-sfGFP-iCre-ERT2

Use

Cre/Lox

Expression

Mammalian

Available Since

March 17, 2020

Availability

Academic Institutions and Nonprofits only

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pHBS1527 [IBB-GFP-mCherry3E]-[BFP-TDP43 W-S]

Plasmid

#133333

Purpose

To test the effect of sequence on TDP43 splicing activity

Depositor

Rajat Rohatgi

Article

Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

Insert

TARDBP mutant (TARDBP Human)

Expression

Mammalian

Mutation

All W to S in CTD of full-length TDP43

Available Since

Nov. 6, 2019

Availability

Academic Institutions and Nonprofits only

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CD4-C-BFP HDRT Source (pTR 176)

Plasmid

#112020

Purpose

DNA sequence source for amplifying an HDR template to tag endogenous human CD4 gene with BFP

Depositor

Alexander Marson

Article

Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

CD4-BFP HDRT (CD4 Human)

Use

CRISPR and Synthetic Biology

Available Since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV-mtagBFP-2A STING V155M 354STOP

Plasmid

#102595

Purpose

Lentivector to express STING V155M delta 354 and mTagBFP

Depositor

Nicolas Manel

Article

Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

Insert

STING (STING1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

V155M delta 354

Promoter

SFFV

Available Since

July 5, 2018

Availability

Academic Institutions and Nonprofits only

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pJM450 EF1alpha EBFP2-P2A-BlastR (TUPV1)

Plasmid

#139230

Purpose

mMoClo TUPV1, with EF1alpha promoter and EBFP2-P2A-BlastR in the MCS

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

EBFP2-P2A-Blasticidin S Deaminase

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1alpha

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pBW768_pCAG-PV1-FRT-lox2272-BFP-BGHpA

Plasmid

#87574

Purpose

PV1 for 2-input, 4-output decoder circuit

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

mtagBFP

Use

Cre/Lox and Synthetic Biology

Expression

Mammalian

Available Since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

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pBABE-tTA-SV40-puro-T2A-TagBFP

Plasmid

#254076

Purpose

Retroviral transfer vector for tTA expression, puromycin selection marker.

Depositor

Jun Huh

Article

Lu et al Cell. 2026 Feb 5;189(3):939-955.e26. doi

Insert

tTA

Use

Retroviral

Available Since

April 13, 2026

Availability

Academic Institutions and Nonprofits only

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aRJ_00134_PGK-mNeonGreen-syn_pA-EF1alpha-TagBFP-syn_pA

Plasmid

#252538

Purpose

Constitutive promoters with downstream tandem syntax for PiggyBac integration

Depositor

Kate Galloway

Article

Johnstone et al bioRxiv [Preprint]. 2025 Jan 19:2025.01.

Insert

TagBFP

Expression

Mammalian

Promoter

hEF1a (human elongation factor 1-alpha), hPGK (hu…

Available Since

April 9, 2026

Availability

Academic Institutions and Nonprofits only

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aRJ_00135_EF1alpha-TagBFP-syn_pA-PGK-mNeonGreen-syn_pA

Plasmid

#252539

Purpose

Constitutive promoters with upstream tandem syntax for PiggyBac integration

Depositor

Kate Galloway

Article

Johnstone et al bioRxiv [Preprint]. 2025 Jan 19:2025.01.

Insert

TagBFP

Expression

Mammalian

Promoter

hEF1a (human elongation factor 1-alpha), hPGK (hu…

Available Since

April 9, 2026

Availability

Academic Institutions and Nonprofits only

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pKG01416_TRE-TagBFP-bGH-EF1alpha-mRuby2-SV40

Plasmid

#252541

Purpose

Inducible gene and constitutive reporter with downstream tandem syntax, used to generate monoclonal PiggyBac-integrated lines

Depositor

Kate Galloway

Article

Johnstone et al bioRxiv [Preprint]. 2025 Jan 19:2025.01.

Insert

mRuby2

Expression

Mammalian

Promoter

hEF1a (human elongation factor 1-alpha), Tight TR…

Available Since

April 9, 2026

Availability

Academic Institutions and Nonprofits only

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pKG01417_TRE-TagBFP-bGH-(EF1alpha-mRuby2-SV40)

Plasmid

#252542

Purpose

Inducible gene and constitutive reporter with convergent syntax, used to generate monoclonal PiggyBac-integrated lines

Depositor

Kate Galloway

Article

Johnstone et al bioRxiv [Preprint]. 2025 Jan 19:2025.01.

Insert

mRuby2

Expression

Mammalian

Promoter

hEF1a (human elongation factor 1-alpha), Tight TR…

Available Since

April 9, 2026

Availability

Academic Institutions and Nonprofits only

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pAJS1222 SOCS4-HA-IRES-Puro-T2A-mTagBFP2

Plasmid

#250756

Purpose

Vector for lentiviral expression of SOCS4-HA with a Puro-T2A-mTAGBFP2 marker

Depositor

Martin Kampmann

Article

Samelson et al Cell. 2026 Jan 28:S0092-8674(25)01487-4.

Insert

SOCS4 (SOCS4 Human)

Use

Lentiviral

Tags

HA

Promoter

CAGG

Available Since

April 2, 2026

Availability

Academic Institutions and Nonprofits only

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pMC-ME_2a-BFP-ORF-1 without ATG

Plasmid

#241162

Purpose

Middle entry vector for mini-Golden subcloning platform; BFP

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

BFP

Use

Mini-golden

Mutation

TCGC linker

Available Since

Feb. 10, 2026

Availability

Academic Institutions and Nonprofits only

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Lenti-CMV-tagBFP-NUDT5 E112Q-WPRE

Plasmid

#250376

Purpose

Lentiviral expression of human NUDT5 carrying E112Q mutation with N-terminal tagBFP under CMV promoter and puromycin resistance cassette under PGK promoter.

Depositor

Stefan Kubicek

Article

Nguyen et al Science. 2025 Nov 6:eadv4257. doi: 10.11

Insert

NUDT5 (NUDT5 Human)

Use

Lentiviral

Tags

tagBFP

Expression

Mammalian

Mutation

E112Q

Promoter

CMV

Available Since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

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pJC2739 - pHR-EF1A-HA-FBXO31-IRES-mTagBFP

Plasmid

#250270

Purpose

cDNA expression of affinity-tagged FBXO31 (HA-FBXO31)

Depositor

Jacob Corn

Article

Muhar et al Nature. 2025 Feb;638(8050):519-527. doi:

Insert

FBXO31 (FBXO31 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

EF-1a

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

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