We narrowed to 6,084 results for: nls

1. pAAV-EF1a-ArgiNLS-mVenus-Q69M(ME)

   Plasmid

   #220597

   Purpose

   Constitutive expression of a single-cell discriminating version of mVenus-Q69M (ME) fluorescent protein.

   Depositor

   Sam Golden

   Article

   Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

   Insert

   mVenus-Q69M (ME)
   

   Use

   AAV

   Tags

   ArgiNLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAAV-CAG-FLEX-ArgiNLS-EGFP

   Plasmid

   #220601

   Purpose

   Cre-dependent expression of a single-cell discriminating version of EGFP fluorescent protein

   Depositor

   Sam Golden

   Article

   Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

   Insert

   EGFP
   

   Use

   AAV and Cre/Lox

   Tags

   ArgiNLS

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. AAVS1-Puro CAG eGFP NLS

   Plasmid

   #170830

   Purpose

   Donor plasmid for knocking eGFP NLS into AAVS1 safe harbor locus

   Depositor

   Xiaoping Bao

   Article

   Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

   Insert

   eGFP
   

   Use

   CRISPR, Synthetic Biology, and TALEN

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pHR-IDR4-mCh-Cry2WT-NLS

   Plasmid

   #221920

   Purpose

   Expression of CBP IDR4 fragment fused to optogenetic protein mCh-Cry2WT

   Depositor

   Daniel Bose

   Insert

   CBP IDR4 (aa 662-787) (CREBBP Human)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   insertion of the IDR4 region (aa 662-787); insert…

   Promoter

   Available since

   July 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pHR-CFID-mCh-Cry2WT-NLS

   Plasmid

   #221923

   Purpose

   Expression of CBP CFID fragment fused to optogenetic protein mCh-Cry2WT

   Depositor

   Daniel Bose

   Insert

   CBP CFID (aa 1892-2441) (CREBBP Human)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   insertion of the CBP-FUS interaction domain (CFID…

   Promoter

   Available since

   July 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pHR-IDR3-mCh-Cry2WT-NLS

   Plasmid

   #221919

   Purpose

   Expression of CBP IDR3 fragment fused to optogenetic protein mCh-Cry2WT

   Depositor

   Daniel Bose

   Insert

   CBP IDR3 (aa 441-587) (CREBBP Human)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   insertion of the IDR3 region (aa 441 - 587); inse…

   Promoter

   Available since

   July 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pHR-IDR7-mCh-Cry2WT-NLS

   Plasmid

   #221922

   Purpose

   Expression of CBP IDR7 fragment fused to optogenetic protein mCh-Cry2WT

   Depositor

   Daniel Bose

   Insert

   CBP IDR7 (aa 2109-2434) (CREBBP Human)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   insertion of the IDR7 region (aa 2109 - 2434); in…

   Promoter

   Available since

   July 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pHR-IDR6-mCh-Cry2WT-NLS

   Plasmid

   #221921

   Purpose

   Expression of CBP IDR6 fragment fused to optogenetic protein mCh-Cry2WT

   Depositor

   Daniel Bose

   Insert

   CBP IDR6 (aa 1856-2090) (CREBBP Human)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   insertion of the IDR6 region (aa 1856-2090); inse…

   Promoter

   Available since

   July 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pHR-IDR1-mCh-Cry2WT-NLS

   Plasmid

   #221918

   Purpose

   Expression of CBP IDR1 fragment fused to optogenetic protein mCh-Cry2WT

   Depositor

   Daniel Bose

   Insert

   CBP IDR1 (aa 72-175) (CREBBP Human)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   insertion of the IDR1 region (aa 72 - 175); inser…

   Promoter

   Available since

   July 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. L1P137 - pAHP6-PhiC31-NLS-tUBQ1

    Plasmid

    #219702

    Purpose

    Level 1 pAHP6-PhiC31-NLS-tUBQ1, for xylem pole specific expression of PhiC31 integrase

    Depositor

    Jennifer Nemhauser

    Insert

    pAHP6:PhiC31-NLS:tUBQ1
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDestTol2-QUAS:NLS-mApple-he1.1:CFP

    Plasmid

    #184814

    Purpose

    Used to generate the Tg(QUAS:NLS-mApple; he1.1:CFP)c718 transgenic line

    Depositor

    Marnie Halpern

    Article

    Spikol et al Elife. 2024 May 31;12:RP89516. doi: 10.7

    Insert

    QUAS:NLS-mApple-pA; he1.1:CFP
    

    Use

    Zebrafish tol2 transgenesis

    Tags

    NLS-mApple

    Expression

    Mutation

    Promoter

    Available since

    May 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDestTol2-QUAS:NLS-GFP-he1.1:CFP

    Plasmid

    #184815

    Purpose

    Used to generate the Tg(QUAS:NLS-GFP; he1.1:CFP)c682 transgenic line

    Depositor

    Marnie Halpern

    Article

    Spikol et al Elife. 2024 May 31;12:RP89516. doi: 10.7

    Insert

    QUAS:NLS-GFP-pA; he1.1:CFP
    

    Use

    Zebrafish tol2 transgenesis

    Tags

    NLS-GFP

    Expression

    Mutation

    Promoter

    Available since

    May 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. YIp128-TET-NUbo-E3(48)-NLS-VSV

    Plasmid

    #212746

    Purpose

    Doxycycline-inducible expression of NUbo-E3(48)-NLS-VSV in budding yeast

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    E3(48)
    

    Use

    Integrative vector

    Tags

    NLS-VSV and NUbo

    Expression

    Yeast

    Mutation

    Promoter

    Tet

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. YIp128-TET-NUbo-E3(63)-NLS-VSV

    Plasmid

    #212756

    Purpose

    Doxycycline-inducible expression of NUbo-E3(63)-NLS-VSV in budding yeast

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    E3(63)
    

    Use

    Integrative vector

    Tags

    NLS-VSV and NUbo

    Expression

    Yeast

    Mutation

    Promoter

    Tet

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. YIp128-ADH-NUbo-E3(63)-NLS-VSV

    Plasmid

    #212755

    Purpose

    Constitutive expression of NUbo-E3(63)-NLS-VSV in budding yeast

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    E3(63)
    

    Use

    Integrative vector

    Tags

    NLS-VSV and NUbo

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. YIp128-ADH-NUbo-E3(48)-NLS-VSV

    Plasmid

    #212745

    Purpose

    Constitutive expression of NUbo-E3(48)-NLS-VSV in budding yeast

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    E3(48)
    

    Use

    Integrative vector

    Tags

    NLS-VSV and NUbo

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. A3Bctd-L7G-Cas9n-UGI-NLS

    Plasmid

    #198886

    Purpose

    Express A3Bctd-L7G BE3-based base editor

    Depositor

    Reuben Harris

    Article

    Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

    Insert

    Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-like 3B C-terminal domain (APOBEC3B Human)
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Loop 7 of A3G

    Promoter

    CMV

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. A3Bctd-D314E-Cas9n-UGI-NLS

    Plasmid

    #198887

    Purpose

    Express A3Bctd-D314E BE3-based base editor

    Depositor

    Reuben Harris

    Article

    Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

    Insert

    Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-like 3B C-terminal domain (APOBEC3B Human)
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    D314E

    Promoter

    CMV

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCMV-NLS-MBP-rATOM(WDR5)

    Plasmid

    #209710

    Purpose

    Mammalian expression of r-ATOM biosensor for WDR5 with nuclear localization signal

    Depositor

    Stewart Loh

    Article

    Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

    Insert

    NLS-MBP-rATOM(WDR5)
    

    Use

    Tags

    NLS-MBP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PET-B-HypaR-SpCas9-NLS-6xHis

    Plasmid

    #207388

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-HypaR-SpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    R661A, N692A, M694A, Q695A, H698A, amino acids 10…

    Promoter

    T7

    Available since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge