Addgene

Showing: 1341 - 1360 of 28186 results

Results per page:

Sort results by:

pLEX-FKBP-Rab7a-Blasticidin

Plasmid

#120716

Purpose

Expresses late endosome-targeted recruiter protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

Rab7a

Use

Lentiviral

Tags

FKBP and mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 30, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
plex_307-3xflag-ccdb-puro

Plasmid

#201122

Purpose

pLEX_307 plasmid with N-terminal FLAG tag and puro marker

Depositor

Alejandro Chavez

Type

Empty backbone

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

EF-1α

Available since

June 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-Tet-MKK6(DD)-Puro

Plasmid

#86094

Purpose

Tet/Dox inducible (TetR) constitutively active mutant MKK6/MAP2K6 in lentiviral vector

Depositor

Cindy Miranti

Article

Frank et al J Cell Sci. 2017 Apr 26. pii: jcs.197152

Insert

MKK6 (MAP2K6 Human)

Use

Lentiviral

Tags

Myc tag

Expression

Mammalian

Mutation

Serine 207 and Threonine 211 both changed to Aspa…

Promoter

CMV/TetO

Available since

Nov. 22, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-BE4GamRA-P2A-Puro

Plasmid

#112673

Purpose

Lentivirus for constitutive expression of BE4GamRA in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

BE4GamRA

Use

Lentiviral

Tags

FLAG

Expression

Mutation

NLS sequence at the N-terminus and D10A

Promoter

EF1s

Available since

July 30, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-xFNLS-P2A-Puro

Plasmid

#110872

Purpose

Lentiviral vector for constitutive expression of xFNLS in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

xFNLS(3.7)

Use

Lentiviral

Tags

3X FLAG

Expression

Mutation

D10A, A262T, R324L, S409I, E480K, E543D, M694I, E…

Promoter

EF1s

Available since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti X2 Zeo DEST (749-3)

Plasmid

#21562

Purpose

3rd gen lentiviral Gateway destination vector, RNAi, 3'LTR insertion, Zeo

Depositor

Eric Campeau

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 22, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-FlaviA-GFP-puro

Plasmid

#140088

Purpose

Fluorescence activatable reporter of flavivirus NS2B-NS3 protease activity

Depositor

Jorge Luis Arias-Arias

Article

Arias-Arias et al J Biol Chem. 2020 Jan 9. pii: RA119.0113

Insert

FlaviA-GFP

Use

Lentiviral; Low expression

Tags

6XHis tag and GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-puro/RAF1-P261A

Plasmid

#131728

Purpose

Mammalian Expression of RAF1

Depositor

Jacques De Grève

Article

Noeparast et al Oncogene. 2019 Aug;38(31):5933-5941. doi

Insert

RAF1 (RAF1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

P261A

Promoter

CMV

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCri_L889-antiSH2-OptoMB-Triple

Plasmid

#159937

Purpose

Opto-MB bacterial expression

Depositor

Jose Avalos, Jared Toettcher

Article

Carrasco-Lopez et al Nat Commun. 2020 Aug 13;11(1):4045. doi:

Insert

anti-SH2 MB with SS58 insert

Use

Tags

Expression

Bacterial

Mutation

AsLOV2 V416I_G528A_N538E

Promoter

Available since

Nov. 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
GNAS_pLENTI-CAG-IRES-GFP

Plasmid

#176991

Purpose

Mammalian lentiviral expression vector encoding GNAS

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

GNAS (GNAS Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CAG

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-ePEG-LINE1-loxPsym

Plasmid

#216866

Purpose

Lentiviral guide RNA vector to insert the loxPsym sequence into LINE1 elements

Depositor

Leopold Parts

Insert

spacerLINE1_cr772scaffold_PBS-loxPsm-HA-tevopreQ

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

Aug. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-FNLS-PGK-Puro

Plasmid

#110844

Purpose

Lentiviral vector for constitutive expression of FNLS in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

BE3RA-FNLS

Use

Lentiviral

Tags

3X FLAG

Expression

Mutation

D10A and NLS sequence at the N-terminus

Promoter

EF1s

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-smURFP-T2A-mCherry

Plasmid

#80347

Purpose

Lentiviral transfer vector under the CMV promoter that expresses smURFP and mCherry.

Depositor

Roger Tsien, Erik Rodriguez

Article

Rodriguez et al Nat Methods. 2016 Aug 1. doi: 10.1038/nm

Inserts

smURFP
mCherry

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CMV

Available since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-CMV-TMPRSS2-TwinStrep_Zeo

Plasmid

#192000

Purpose

Lentiviral vector to generate TwinStrep-tagged TMPRSS2 stable expressing cell line

Depositor

Jan Carette

Article

Richards et al Science. 2022 Sep 8:eabn5648. doi: 10.11

Insert

TMPRSS2 (TMPRSS2 Human)

Use

Lentiviral

Tags

TMPRSS2

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-eGFP-dSaCas9-blast

Plasmid

#191653

Purpose

Lentiviral expression of S. aureus dead Cas9 (dCas9/dSaCas9/SadCas9), eGFP, and the blasticidin resistance gene.

Depositor

Moshe Szyf

Article

Sapozhnikov et al Sci Rep. 2023 Feb 9;13(1):2280. doi: 10.

Insert

eGFP-T2A-dSaCas9

Use

CRISPR and Lentiviral

Tags

3XFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-CaMKIIa-Archer1-EGFP

Plasmid

#60423

Purpose

Lentiviral vector with CaMKIIa promoter and upstream CMV promoter expressing Archer1-EGFP fusion. Archer1's red fluorescence monitors changes in membrane voltage.

Depositor

Viviana Gradinaru

Article

Flytzanis et al Nat Commun. 2014 Sep 15;5:4894. doi: 10.

Insert

Archer1-EGFP

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CaMKIIa

Available since

Nov. 4, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-xF2X-P2A-Puro

Plasmid

#110873

Purpose

Lentiviral vector for constitutive expression of xFNLS in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

xFNLS-2X(3.7)

Use

Lentiviral

Tags

3X FLAG

Expression

Mutation

D10A, A262T, R324L, S409I, E480K, E543D, M694I, E…

Promoter

EF1s

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti X2 Hygro DEST (w17-1)

Plasmid

#17295

Purpose

3rd gen lentiviral Gateway destination vector, RNAi, 3’LTR insertion, Hygro

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-xBE4Gam-P2A-Puro

Plasmid

#112676

Purpose

Lentivirus for constitutive expression of xCas9(3.7)-BE4Gam in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

xCas9(3.7)-BE4Gam

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

D10A, A262T, R324L, S409I, E480K, E543D, M694I, E…

Promoter

EF1s

Available since

July 30, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
plenti hSyn PGK1-HALO

Plasmid

#220910

Purpose

Neuronal specific expression of the fusion construct PGK1-HALO

Depositor

Timothy Ryan

Article

Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

Insert

PGK1-HALO (PGK1 Human)

Use

Lentiviral

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

June 14, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge