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pENTR2b-PTPN11(D425A,C459S)

Plasmid

#215515

Purpose

Gateway entry vector with a phosphatase-inactive Shp2 mutant (D425A,C459S)

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

PTPN11 (PTPN11 Human)

Use

Gateway Cloning

Mutation

D425A, C459S (Phosphatase inactive)

Promoter

none

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pENTR2b-ITGB1(WT)

Plasmid

#215450

Purpose

Gateway entry vector with integrin beta1 wild-type (WT)

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

ITGB1 (ITGB1 Human)

Use

Gateway Cloning

Mutation

Silent mutations added to disrupt shRNA binding a…

Promoter

none

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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p5E-Dll4-F2-E1B-B-Globin (JDW 1366)

Plasmid

#229843

Purpose

A gateway compatible 5' entry clone containing the murine Dll4 F2 arterial specific enhancer upstream of a minimal E1b promoter and intron for arterial and endocardial expression.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Dll4-F2 (exon3)

Use

Gateway Cloning

Available Since

March 28, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR1A 2xHA GPR153

Plasmid

#226808

Purpose

Gateway entry vector for 2xHA GPR153

Depositor

Ramiro Iglesias-Bartolome

Article

Pedro et al J Invest Dermatol. 2024 Jun;144(6):1311-

Insert

GPR153 (GPR153 Human)

Use

Gateway Cloning

Tags

2xHA tag followed by a GDPPVAT linker

Mutation

Sequence optimized from NP_997253.2

Available Since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR1A 2xHA LTB4R

Plasmid

#226806

Purpose

Gateway entry vector for 2xHA LTB4R

Depositor

Ramiro Iglesias-Bartolome

Article

Pedro et al J Invest Dermatol. 2024 Jun;144(6):1311-

Insert

LTB4R (LTB4R Human)

Use

Gateway Cloning

Tags

2xHA tag followed by a GDPPVAT linker

Mutation

Sequence optimized from NP_858043.1

Available Since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pME-LexA-mODC-LexOP-35SCaMV (JDW 1348)

Plasmid

#224505

Purpose

Gateway compatible middle entry clone containing a Destablized LexA and LexOperon with minimal CaMV 35S promoter as an all in one casette for RU486 driven gene expression

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

LexA-mODC-LexOP-35SCaMV

Use

Gateway Cloning

Available Since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pME-LexA-mODC-LexOP-c-Fos (JDW 1359)

Plasmid

#224506

Purpose

Gateway compatible middle entry clone containing a Destablized LexA and LexOperon with 3' minimal c-Fos promoter for an all in one RU486 driven gene expression construct

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

LexA-oMDC-LexOP-c-Fos

Use

Gateway Cloning

Available Since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pME-3xHA-BirA-T2A-mCherry-caax (JDW 889)

Plasmid

#224512

Purpose

A Gateway compatible middle entry clone containing Biotin Ligase followed by T2A and mCherry membrane reporter (biotinylation and flourescent cell membrane reporter)

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

3xHA-BirA-T2A-mCherry-Caax

Use

Gateway Cloning

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pME-lox-3xNLS-mCherry-V5-SV40pA-3xstop-lox (JDW 1232)

Plasmid

#224522

Purpose

A Gateway compatible middle entry clone containing loxP flanked 3xNLS-mCherry-V5-3xSTOP cassette

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

lox-3xNLS-mCherry-V5-SV40pA-3xstop-lox

Use

Cre/Lox

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR-SLC66A5_STOP

Plasmid

#221422

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC66A5 (MPDU1 Human)

Expression

Mammalian

Available Since

July 18, 2024

Availability

Academic Institutions and Nonprofits only

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