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YIp128-TET-NUbo-E3(48)-NLS-VSV

Plasmid

#212746

Purpose

Doxycycline-inducible expression of NUbo-E3(48)-NLS-VSV in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(48)

Use

Integrative vector

Tags

NLS-VSV and NUbo

Expression

Yeast

Promoter

Tet

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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YIp128-TET-NUbo-E3(63)-NLS-VSV

Plasmid

#212756

Purpose

Doxycycline-inducible expression of NUbo-E3(63)-NLS-VSV in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(63)

Use

Integrative vector

Tags

NLS-VSV and NUbo

Expression

Yeast

Promoter

Tet

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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YIp128-CUP1-His6-CUbo(K48R)-GFP

Plasmid

#212793

Purpose

Copper-inducible expression of the artificial test substrate His6-CUbo(K48R)-GFP in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

His6-CUbo(K48R)-GFP

Use

Integrative vector

Expression

Yeast

Mutation

CUb(K48R)

Promoter

CUP1

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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YIp128-CUP1-His6-CUbo(K63R)-GFP

Plasmid

#212794

Purpose

Copper-inducible expression of the artificial test substrate His6-CUbo(K63R)-GFP in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

His6-CUbo(K63R)-GFP

Use

Integrative vector

Expression

Yeast

Mutation

CUb(K63R)

Promoter

CUP1

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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YIp128-CUP1-His6-CUbo(K48R/K63R)-GFP

Plasmid

#212795

Purpose

Copper-inducible expression of the artificial test substrate His6-CUbo(K48R/K63R)-GFP in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

His6-CUbo(K48R/K63R)-GFP

Use

Integrative vector

Expression

Yeast

Mutation

CUb(K48R/K63R)

Promoter

CUP1

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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YIp211-CUP1-NUbo-E3(63) I227A-VSV

Plasmid

#212757

Purpose

Copper-inducible expression of NUbo-E3(63) I227A-VSV in budding yeast, reduced activity of E3(63)

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(63) I227A

Use

Integrative vector

Tags

NUbo and VSV

Expression

Yeast

Mutation

Pib1(I227A)

Promoter

CUP1

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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YIp211-ADH-NUbo-E3(48) aa24-150-VSV

Plasmid

#212747

Purpose

Constitutive expression of NUbo-E3(48)ΔU7BD-VSV in budding yeast, inactive E3(48)

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(48) aa24-150

Use

Integrative vector

Tags

NUbo and VSV

Expression

Yeast

Mutation

Cue1 ΔU7BD

Promoter

ADH1

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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YIp211-ADH-myc-E3(1)-CUbo-mCherry-VSV

Plasmid

#212740

Purpose

Consitutive expression of myc-E3(1)-CUbo-mCherry-VSV in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(1)

Use

Integrative vector

Tags

CUbo-mCherry-VSV and myc

Expression

Yeast

Promoter

ADH1

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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YIp128-ADH-myc-E3(1)-CUbo-mCherry-VSV

Plasmid

#212739

Purpose

Consitutive expression of myc-E3(1)-CUbo-mCherry-VSV in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(1)

Use

Integrative vector

Tags

CUbo-mCherry-VSV and myc

Expression

Yeast

Promoter

ADH1

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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YIp211-ADH-myc-E3(1) C885A-CUbo-VSV

Plasmid

#212738

Purpose

Consitutive expression of myc-E3(1) C885A-CUbo-VSV in budding yeast, catalytic inactive E3(1)

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(1)

Use

Integrative vector

Tags

CUbo-NLS-VSV and myc

Expression

Yeast

Mutation

HOIP(C885A)

Promoter

ADH1

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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