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pEJS2194-RP_PiggyBac_SpCas9ABE8e-tracrRNA-Puro

Plasmid

#211821

Purpose

PiggyBac vector expressing SpCas9-ABE8e with tracrRNA for stable cell line establishment

Depositor

Erik Sontheimer

Article

Zhang et al Nucleic Acids Res. 2023 Nov 30:gkad1125.

Insert

SpCas9-ABE8e, tracrRNA and Puromycin resistance gene

Expression

Mammalian

Promoter

chicken β-actin promoter, U6 promoter and hPGK pr…

Available Since

Jan. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pEJS-HZ08-pAAVsc.U6-tracrRNA

Plasmid

#211816

Purpose

AAV vector expressing tracrRNA under U6 promoter

Depositor

Erik Sontheimer

Article

Zhang et al Nucleic Acids Res. 2023 Nov 30:gkad1125.

Insert

U6-tracrRNA

Use

AAV

Expression

Mammalian

Promoter

U6

Available Since

Jan. 10, 2024

Availability

Academic Institutions and Nonprofits only

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TRAPa(R150A)-3xFLAG-pCDNA

Plasmid

#201966

Purpose

Mammalian expression of mutated Translocon-associated protein subunit alpha

Depositor

Ville Paavilainen

Article

Karki et al EMBO Rep. 2023 Dec 6;24(12):e57910. doi:

Insert

SSR1 (SSR1 Human)

Tags

3xFLAG-tag

Expression

Mammalian

Mutation

R150A

Available Since

Jan. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmTral-ADF_E

Plasmid

#146206

Purpose

Insect Expression of DmTral-ADF

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

Insert

DmTral-ADF (tral Fly)

Expression

Insect

Mutation

three silent mutations A198C, A387G, A1437G and 4…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmTral-FAF_E

Plasmid

#146207

Purpose

Insect Expression of DmTral-FAF

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

Insert

DmTral-FAF (tral Fly)

Expression

Insect

Mutation

three silent mutations A198C, A387G, A1437G and 4…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmTral-FDA_E

Plasmid

#146208

Purpose

Insect Expression of DmTral-FDA

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

Insert

DmTral-FDA (tral Fly)

Expression

Insect

Mutation

three silent mutations A198C, A387G, A1437G and 4…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmTral-S13AI15AD30KT35A_E

Plasmid

#146217

Purpose

Insect Expression of DmTral-S13AI15AD30KT35A

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

Insert

DmTral-S13AI15AD30KT35A (tral Fly)

Expression

Insect

Mutation

two silent mutations A198C, A387G and a mutation …

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmTral-S13AI15AR21A_E

Plasmid

#146218

Purpose

Insect Expression of DmTral-S13AI15AR21A

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

Insert

DmTral-S13AI15AR21A (tral Fly)

Expression

Insect

Mutation

two silent mutations A198C, A387G and a mutation …

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmTral-R21A_E

Plasmid

#146220

Purpose

Insect Expression of DmTral-R21A

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

Insert

DmTral-R21A (tral Fly)

Expression

Insect

Mutation

two silent mutations A198C, A387G and a mutation …

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pETM-60-NusA-DmTral_1-84_C

Plasmid

#146082

Purpose

Bacterial Expression of DmTral_1-84

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

Insert

DmTral_1-84 (tral Fly)

Expression

Bacterial

Mutation

one silent mutation A198C compared to the sequenc…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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