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Plasmid#213525PurposeExpresses NSP14 of SARS-CoV-2 with a single point mutation P335HDepositorInsertSARS-CoV-2 NSP14 (ORF1ab SARS-CoV-2)
UseTagsHis6-sumoExpressionBacterialMutationchanged P335 to HPromoterAvailable sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSF_his6_sumo_SARS_CoV2_NSP14_H424R
Plasmid#213526PurposeExpresses NSP14 of SARS-CoV-2 with a single point mutation H424RDepositorInsertSARS-CoV-2 NSP14 (ORF1ab SARS-CoV-2)
UseTagsHis6-sumoExpressionBacterialMutationchanged H424 to RPromoterAvailable sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pAAV-Syn-FLEX-rc[ChrimsonR-tdTomato]
Plasmid#62723PurposeAAV-mediated expression of ChrimsonR-tdTomato under the Syn promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal. TdTomato is a codon diversified version.DepositorHas ServiceAAV1 and AAV5InsertChrimsonR-tdTomato
UseAAVTagstdTomatoExpressionMammalianMutationChrimson K176R mutantPromoterhuman synapsin promoterAvailable sinceApril 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1α1.1-ChrimsonR-tdTomato
Plasmid#122064PurposeAAV-mediated expression of ChrimsonR-tdTomato under the EF1α promoter (1.1kb short version).tdTomato has codons varied to reduce recombination. Using SV40 pA signal.DepositorInsertChrimsonR-tdTomato
UseAAVTagstdTomato (codon diversified version)ExpressionMammalianMutationChrimson K176R mutantPromoterEF1a(1.1kb short version)Available sinceNov. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-FLEX-fd [ChrimsonR-tdTomato]
Plasmid#84483PurposeAAV-mediated expression of ChrimsonR-tdTomato under the Syn promoter, in floxed/forward (Cre-dependent) manner. Cre turns gene off. Using bGHpA signal. tdTomato is a codon diversified version.DepositorInsertChrimsonR-tdTomato
UseAAVTagstdTomatoExpressionMammalianMutationChrimson K176R mutantPromoterhuman synapsin promoterAvailable sinceApril 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
AAV-EF1α1.1-FLPX-rc [ChrimsonR-tdTomato]
Plasmid#128587PurposeAAV-mediated expression of ChrimsonR-tdTomato under the EF1α1.1 promoter in frt/reversed (Flp-dependent) manner. tdTomato has codons varied to reduce recombination.DepositorInsertChrimsonR-tdTomato
UseAAVTagstdTomato (codon diversified version)ExpressionMammalianMutationChrimson K176R mutantPromoterEF1α (1.1 kb short version)Available sinceSept. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCS-TRE-FHDnd1-Ubc-rtTA-I2G
Plasmid#70076Purpose2nd gen lentiviral vector. tet/dox controllable expression of FLAG-HA-tagged murine Dnd1 in mammalian cellsDepositorInsertDnd1 (Dnd1 Mouse)
UseLentiviralTagsFLAG and HAExpressionMammalianMutationSilent mutations are introduced to escape from RN…PromoterTet responsible promoterAvailable sinceNov. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDCAF3-IS5
Plasmid#65220PurposeExpresses N-demethylases for the degredation of caffeine and related methylxanthines. Can be used to measure caffeine content as described in the publication listed below.DepositorInsertsndmA
ndmB
ndmC
ndmD
gst9
chlR
UseSynthetic BiologyTagsExpressionBacterialMutationChanged start codon from gtg to atg and Changed s…PromoterBBa_J23100Available sinceJan. 8, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCS-TRE-FHDnd1-Y102A-Ubc-rtTA-I2G
Plasmid#70075Purpose2nd gen lentiviral vector. tet/dox controllable expression of FLAG-HA-tagged murine Dnd1 (RRM mutant) in mammalian cellsDepositorInsertDnd1 (Dnd1 Mouse)
UseLentiviralTagsFLAG and HAExpressionMammalianMutationY102A. Silent mutations are introduced to escape …PromoterTet responsible promoterAvailable sinceNov. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGLU_66
Plasmid#225769PurposeThis is the native promoter from the catP chloramphenicol resistance cassette.DepositorInsertPcatP_[abr]
UseSynthetic BiologyTagsExpressionMutationN/APromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC-L3L2
Plasmid#114019PurposeCreate Entry clones for Gateway LR reactions through simple restriction enzyme based cloningDepositorInsertccdB gene, Chloramphenicol resistance gene
UseGatewayTagsExpressionMutationPromoterAvailable sinceJan. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC-L1L4
Plasmid#114018PurposeCreate Entry clones for Gateway LR reactions through simple restriction enzyme based cloningDepositorInsertccdB gene, Chloramphenicol resistance gene
UseGatewayTagsExpressionMutationPromoterAvailable sinceJan. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC-L1L2
Plasmid#114017PurposeCreate Entry clones for Gateway LR reactions through simple restriction enzyme based cloningDepositorInsertccdB gene, Chloramphenicol resistance gene
UseGatewayTagsExpressionMutationPromoterAvailable sinceJan. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBD026
Plasmid#201309PurposeAvrSr27-2 as Golden Gate compatible MoClo CDS partDepositorInsertAvrSr27-2
UseTagsExpressionPlantMutationremoval of BsaI and BpiI sitesPromoterAvailable sinceOct. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBD025
Plasmid#201308PurposeAvrSr27-1 as Golden Gate compatible MoClo CDS partDepositorInsertAvrSr27-1
UseTagsExpressionPlantMutationremoval of BsaI and BpiI sitesPromoterAvailable sinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSB4A3-PBAD-phzO
Plasmid#165619PurposeArabinose-inducible expression of phzO for conversion of phenazine-1-carboxylic acid to 2-hydroxyphenazineDepositorInsertphzO
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPBADAvailable sinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_50_CA_repeat
Plasmid#65869PurposeCarries CA repeats of 50 nucleotides in length, an RBPMS targetDepositorInsertCA repeats 50 nucleotides in lngth
UseTagsExpressionMutationPromoterT7Available sinceJuly 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_50_100_CA_repeat
Plasmid#65867PurposeCarries CA repeats of 50-100 nucleotides in length, an RBPMS targetDepositorInsertCA repeats 50-100 nucleotides in lngth
UseTagsExpressionMutationPromoterT7Available sinceJune 23, 2015AvailabilityAcademic Institutions and Nonprofits only