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pIVT/Smarcc1

Plasmid

#122145

Purpose

For making Smarcc1 RNA

Depositor

Carmen Williams

Article

Miao et al Biol Reprod. 2018 Jan 9. pii: 4794936. d

Insert

Smarcc1 (Smarcc1 Mouse)

Use

For in vitro transcription

Promoter

T7

Available Since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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f-CTS-EGFP

Plasmid

#86064

Purpose

A mammalian expression plasmid encoding fibrocystin ciliary targeting signal domain with C-terminus EGFP.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

Fibrocystin (Pkhd1 Mouse)

Tags

GFP

Expression

Mammalian

Mutation

contains ciliary targeting signal (3870-3887 amin…

Promoter

CMV

Available Since

May 8, 2017

Availability

Academic Institutions and Nonprofits only

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pLYS5-MCU-Flag

Plasmid

#50054

Purpose

Expression of human MCU in mammalian cells

Depositor

Vamsi Mootha

Article

Sancak et al Science. 2013 Nov 14.

Insert

MCU-Flag (MCU Human)

Tags

Flag

Expression

Mammalian

Available Since

Jan. 21, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV-Neo-Bam E2F6

Plasmid

#37965

Depositor

Jacqueline Lees

Article

Trimarchi et al Proc Natl Acad Sci U S A. 2001 Feb 13;98

Insert

E2F6 (E2F6 Human)

Expression

Mammalian

Available Since

July 18, 2012

Availability

Academic Institutions and Nonprofits only

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pCAG-OSF-VPS15

Plasmid

#99326

Purpose

Component for mammalian expression of recombinant PI3KC3-C1 complex

Depositor

James Hurley

Article

Baskaran et al Elife. 2014 Dec 9;3. doi: 10.7554/eLife.

Insert

VPS15 (PIK3R4 Human)

Tags

Strep-Strep-Flag

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 21, 2017

Availability

Academic Institutions and Nonprofits only
AAV-hSyn-evoCjCas9-ABE8e_gRNA

Plasmid

#202561

Purpose

hSyn-evoCjCas9-ABE8e and hU6-BsmBI-gRNA in AAV2 backbone (ITRs)

Depositor

Gerald Schwank

Article

Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

Insert

evoCjCas9-ABE8e

Use

AAV

Available Since

Sept. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA-Dest47- C11orf83-GFP

Plasmid

#65844

Purpose

Mammalian expression of C terminally GFP-tagged C11orf83/UQCC3

Depositor

Amos Bairoch

Article

Desmurs et al Mol Cell Biol. 2015 Apr;35(7):1139-56. d

Insert

UQCC3 WT (UQCC3 Human)

Tags

GFP

Expression

Mammalian

Mutation

WT

Available Since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

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pLYS1-EMRE/RNAi resistant

Plasmid

#50056

Purpose

Expression of human EMRE in mammalian cells with silent mutation making it resistant to RNAi

Depositor

Vamsi Mootha

Article

Sancak et al Science. 2013 Nov 14.

Insert

EMRE-RNAi resistant (SMDT1 Human)

Expression

Mammalian

Mutation

RNAi resistant, silent mutant

Available Since

Feb. 24, 2014

Availability

Academic Institutions and Nonprofits only

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pST39-Hrs-STAM1 core

Plasmid

#21497

Depositor

James Hurley

Article

Ren et al Structure. 2009 Mar 11. 17(3):406-16.

Insert

pST39- Hrs(398-514)-His/STAM1(301-387) (STAM, HGS Human)

Tags

His

Expression

Bacterial

Mutation

none

Available Since

Feb. 1, 2010

Availability

Academic Institutions and Nonprofits only
pIVT/Smarca4

Plasmid

#122146

Purpose

For making Smarca4 RNA

Depositor

Carmen Williams

Article

Miao et al Biol Reprod. 2018 Jan 9. pii: 4794936. d

Insert

Smarca4 (Smarca4 Mouse)

Use

For in vitro transcription

Promoter

T7

Available Since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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FLAG-hIes6

Plasmid

#15356

Depositor

Joan Conaway, Ronald Conaway

Article

Jin et al J Biol Chem. 2005 Dec 16. 280(50):41207-

Insert

hIes6 (INO80C Human)

Tags

FLAG

Expression

Mammalian

Available Since

Aug. 17, 2007

Availability

Academic Institutions and Nonprofits only
pF208wt-luc

Plasmid

#122143

Purpose

For luciferase expression

Depositor

Carmen Williams

Article

Miao et al Biol Reprod. 2018 Jan 9. pii: 4794936. d

Insert

Fam208a promoter (Tasor Mouse)

Use

Luciferase

Promoter

Fam208a

Available Since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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MAC_C_TGON2

Plasmid

#111677

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

Insert

TGON2 (TGOLN2 Human)

Expression

Mammalian

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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FLAG-FLJ90652

Plasmid

#15359

Depositor

Joan Conaway, Ronald Conaway

Article

Jin et al J Biol Chem. 2005 Dec 16. 280(50):41207-

Insert

FLJ90652 (INO80E Human)

Tags

FLAG

Expression

Mammalian

Available Since

Aug. 17, 2007

Availability

Academic Institutions and Nonprofits only

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JW617

Plasmid

#64809

Purpose

CUC3 expression in yeast

Depositor

Detlef Weigel

Article

Rubio-Somoza et al Curr Biol. 2014 Nov 17;24(22):2714-9. do

Insert

CUC3 (CUC3 Mustard Weed)

Tags

GAL4 DNA binding domain and Myc

Available Since

Aug. 26, 2015

Availability

Academic Institutions and Nonprofits only

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JW624

Plasmid

#64808

Purpose

CUC2 expression in yeast

Depositor

Detlef Weigel

Article

Rubio-Somoza et al Curr Biol. 2014 Nov 17;24(22):2714-9. do

Insert

CUC2 (CUC2 Mustard Weed)

Tags

GAL4 DNA binding domain

Available Since

Sept. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pmcsg7-mCRT(WT)

Plasmid

#83505

Purpose

Bacterial expression of Calreticulin

Depositor

Malini Raghavan

Article

Del Cid et al J Biol Chem. 2010 Feb 12;285(7):4520-35.

Insert

CALR (Calr Mouse)

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 12, 2016

Availability

Academic Institutions and Nonprofits only
LifeAct-TagBFP-DiB-RM

Plasmid

#168782

Purpose

Actin filament reporter, LifeAct, fused with blue fluorescent protein TagBFP and fluorogen activating protein (FAP) DiB-RM

Depositor

Jens Meiler

Article

Bozhanova et al PLoS Comput Biol. 2021 Nov 8;17(11):e100

Insert

LifeAct-TagBFP-DiB-RM

Expression

Mammalian

Promoter

CMV

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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YFP-Gamma 5

Plasmid

#36044

Depositor

Narasimhan Gautam

Article

Akgoz et al Cell Signal. 2006 Oct;18(10):1758-68. Ep

Insert

G protein subunit Gamma 5 (Gng5 Rat)

Tags

His and YFP

Expression

Mammalian

Promoter

CMV

Available Since

March 22, 2013

Availability

Academic Institutions and Nonprofits only
pBCC155-MXMT

Plasmid

#79816

Purpose

CFP-based BiFC vector for plants (Positive control: MXMT)

Depositor

Yutaka Kodama, Masamitsu Wada

Article

Kodama et al Plant Mol Biol. 2009 May;70(1-2):211-7.

Insert

CaMXMT1

Use

Reporter plasmid

Tags

CC155/CFP (155–239)

Expression

Plant

Promoter

CaMV 35S promoter and CaMV35S

Available Since

Dec. 2, 2016

Availability

Academic Institutions and Nonprofits only

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