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pKLV-EF1aGFP2AKAT7(E508Q)-W

Plasmid

#159293

Purpose

Lentiviral vector expressing GFP and KAT7 (gRNA-resistant, E508Q mutant) driven by human EF1a promoter

Depositor

Kosuke Yusa

Article

Au et al Leukemia. 2021 Apr;35(4):1012-1022. doi:

Insert

KAT7 E508Q

Use

Lentiviral

Expression

Mammalian

Mutation

CRISPR sites mutated (gRNA ID. 5 and A10) and E50…

Promoter

human EF1a promoter

Available Since

Dec. 3, 2020

Availability

Academic Institutions and Nonprofits only

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hSyn-BiPOLES-mCerulean

Plasmid

#154944

Purpose

Optogenetic tool for blue-light inhibition and red-light excitation of neurons

Depositor

Simon Wiegert

Article

Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

Insert

BiPOLES

Use

AAV

Expression

Mammalian

Promoter

human synapsin

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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p2R3a-nosT

Plasmid

#71272

Purpose

Entry clone containing nosT. Necessary to complete the transcriptional reporters by providing the poly-A tail. For use in plants and compatible with the MultiSite Gateway system

Depositor

Ari Pekka Mähönen

Article

Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

Insert

nopaline synthase terminator

Use

Gateway

Available Since

Dec. 7, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV EF1a DIO iChloC 2A dsRed

Plasmid

#70762

Purpose

An improved chloride-conducting channelrhodopsin for light-induced inhibition of neuronal activity in vivo. Cre inducible expression (double floxed inversed ORF)

Depositor

Thomas Oertner

Article

Wietek et al Sci Rep. 2015 Oct 7;5:14807. doi: 10.103

Inserts

Channelrhodopsin-2
red fluorescent protein

Use

AAV

Expression

Mammalian

Mutation

E83Q,E90R,E101S,D156N,T159C

Promoter

EF1a

Available Since

Jan. 7, 2016

Availability

Academic Institutions and Nonprofits only

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Il12b 400bp pro-Luc

Plasmid

#20020

Depositor

Stephen Smale

Article

Plevy et al Mol Cell Biol. 1997 Aug . 17(8):4572-88.

Insert

Il12b promoter (Il12b Mouse)

Use

Luciferase

Expression

Mammalian

Available Since

Jan. 28, 2009

Availability

Academic Institutions and Nonprofits only

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p2R3a-TagRFP-OcsT

Plasmid

#71270

Purpose

Entry clone containing TagRFP. Can be fused with linker to the C-terminal end of protein of interest. For use in plants and compatible with the MultiSite Gateway system

Depositor

Ari Pekka Mähönen

Article

Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

Insert

TagRFP

Use

Gateway

Tags

4xGly linker and octaline synthase terminator

Available Since

Dec. 7, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-C1V1 (t/t)-TS-mCherry

Plasmid

#35500

Purpose

AAV expression of CaMKII-driven chimeric opsin variant C1V1 (E122T/E162T) for fast and potent optical excitation at red-shifted wavelengths.

Depositor

Karl Deisseroth

Article

Yizhar et al Nature. 2011 Jul 27;477(7363):171-8. doi

Insert

ChR1-VChR1 Chimera

Use

AAV

Tags

mCherry

Expression

Mammalian

Mutation

E122T and E162T

Promoter

CaMKIIa

Available Since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

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mCer-4aa-VAMP2

Plasmid

#53233

Purpose

Fluorescent probe: VAMP2 (synaptobrevin2), N-terminally-tagged with monomeric Cerulean, connected with a 4 amino acid linker. Expressed in mammalian cells, used with mCit-4aa-SNAP25B for FRET.

Depositor

Robert Zucker

Article

Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.

Insert

VAMP2 (Vamp2 Rat)

Tags

4aa-linker and mCerulean

Expression

Mammalian

Available Since

June 9, 2014

Availability

Academic Institutions and Nonprofits only
J23104-mRFP1-331Bb

Plasmid

#78274

Purpose

mRFP1 behind constitutive promoter J23104 in pSEVA331Bb backbone

Depositor

Tom Ellis

Article

Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

Insert

J23104-mRFP1

Available Since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pFBD-Akt13C(S473D)

Plasmid

#86577

Purpose

Expresses cleavable human full-length Akt1(S473D) phosphomimetic

Depositor

Thomas Leonard, Ivan Yudushkin

Article

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

Akt1 (AKT1 Human)

Tags

10xHis

Expression

Insect

Mutation

3C cleavage site inserted at pos. 130; S473D phos…

Available Since

April 4, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-hChR2(C128S/D156A)-EYFP

Plasmid

#35501

Purpose

AAV expression of CaMKII-driven stabilized step function opsin (SSFO) for optogenetic activation.

Depositor

Karl Deisseroth

Article

Yizhar et al Nature. 2011 Jul 27;477(7363):171-8. doi

Insert

hChR2

Use

AAV

Tags

EYFP

Expression

Mammalian

Mutation

C128S and D156A

Promoter

CaMKIIa

Available Since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

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ESR1-luciferase

Plasmid

#113351

Purpose

evaluate FOXA1 DIV motif enhancer activity near 50 kb of ESR1 gene

Depositor

Ralf Jauch

Article

Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

Insert

ESR1-enhancer (ESR1 Human)

Use

Luciferase

Available Since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pCEP4-HA-Flag-natT1R2 FS1

Plasmid

#113955

Purpose

mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variant

Depositor

Erik Procko

Article

Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

Insert

T1R2 (TAS1R2 Human)

Tags

FLAG and Signal/leader sequence from influenza he…

Expression

Mammalian

Mutation

delete N-terminal 21 residues, one base pair dele…

Promoter

cmv

Available Since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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hMLKL-Venus-Plasma

Plasmid

#106081

Purpose

expression in mammalian cells

Depositor

Douglas Green

Article

Quarato et al Mol Cell. 2016 Feb 18;61(4):589-601. doi

Insert

hMLKL-Venus-Plasma (MLKL Human)

Use

Retroviral; Tet-on retrovirus

Tags

plasma membrane-targetting domain of H-Ras (aa 1-…

Expression

Mammalian

Promoter

dox-inducible

Available Since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-CamkII-bPac(WT)-mCherry-minWPRE.sbd

Plasmid

#118278

Purpose

Expresses bPAC(WT)-mCherry under a minimal CamKII Promoter

Depositor

Dietmar Schmitz

Article

Bernal Sierra et al Nat Commun. 2018 Nov 5;9(1):4611. doi: 1

Insert

bPAC(WT)

Use

AAV

Tags

mCherry and myc

Promoter

CamKII

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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pGL4.23_Promoter_1-3

Plasmid

#210629

Purpose

Luciferase experiment for TAL1 promoter 1-3

Depositor

Maayan Salton

Article

Sharma et al PLoS Biol. 2023 Jun 28;21(6):e3002175. d

Insert

promoter 1-3 (TAL1 Human)

Expression

Mammalian

Promoter

Minimal Promoter

Available Since

Jan. 9, 2024

Availability

Academic Institutions and Nonprofits only

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mDlx-BiPOLES-mCerulean

Plasmid

#154946

Purpose

Optogenetic tool for blue-light inhibition and red-light excitation of neurons

Depositor

Simon Wiegert

Article

Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

Insert

BiPOLES

Use

AAV

Expression

Mammalian

Promoter

minimal Dlx

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CamKII-GFP-P2A-bReaches-minWPRE

Plasmid

#118276

Purpose

Expresses EGFP and bReaChES under a minimal CamKII Promoter

Depositor

Dietmar Schmitz

Article

Bernal Sierra et al Nat Commun. 2018 Nov 5;9(1):4611. doi: 1

Inserts

EGFP
bReaCHES

Use

AAV

Tags

P2A

Promoter

CamK II

Available Since

Dec. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-HLA-cmyc-natT1R2

Plasmid

#113946

Purpose

mammalian expression plasmid for c-myc-tagged human T1R2 with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain

Depositor

Erik Procko

Article

Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

Insert

T1R2 (TAS1R2 Human)

Tags

Signal/leader sequence from HLA class I histocomp…

Expression

Mammalian

Mutation

residues 22-839

Available Since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pGL4.23_Promoter_4

Plasmid

#210630

Purpose

Luciferase experiment for TAL1 promoter 4

Depositor

Maayan Salton

Article

Sharma et al PLoS Biol. 2023 Jun 28;21(6):e3002175. d

Insert

promoter 4 (TAL1 Human)

Expression

Mammalian

Promoter

Minimal Promoter

Available Since

June 10, 2024

Availability

Academic Institutions and Nonprofits only

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