Skip to main content
Addgene
Showing: 121 - 140 of 12614 results

  7. EK0438 SFFV-mCherry-SGc(s70587)cSG-EGFP-(3xMS2) (FLP-IN)

    Plasmid
    #191163
    Purpose
    Expression of a sensor for synthetic sequence 1 (EK0208) with three MS2 sequences in the 3' UTR in mammalian cells. Compared to EK0285, also has additional restriction sites around the sensor sequence. Recommended for the basis for cloning new sensors for use with MCP-ADAR(DD). HindIII & MluI can be used to swap out marker (mCherry). (KpnI or BamHI) and (BshTI or BcuI) can be used to swap out sensor sequence (s70587). XhoI and ApaI can be used to swap out output (EGFP).
    Depositor
    Xiaojing Gao
    Article
    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038
    Insert
    mCherry:s70587:EGFP-3xMS2
    Use
    Tags
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    SFFV
    Available since
    Oct. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    191163-plasmid-map-sequence-id-375409

    Enlarge

  8. pRL838

    Plasmid
    #70680
    Purpose
    This vector was used to generate a library of ca. 17-kb sequences of clones as part of a genomic sequence strategy and were then used to complement mutations.
    Depositor
    Peter Wolk
    Article
    Kaneko et al DNA Res. 2001 Oct 31;8(5):205-13; 227-53
    Type
    Empty backbone
    Use
    Cre/Lox
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    70680-plasmid-map-sequence-id-126382

    Enlarge

  10. pGL003

    Plasmid
    #215335
    Purpose
    amyE integration vector for IPTG-inducible expression in Bacillus subtilis (Pspank promoter)
    Depositor
    Gene-Wei Li
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pSpank
    Available since
    March 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    215335-plasmid-map-sequence-id-426095

    Enlarge

  11. pAG497 mito-iFAST

    Plasmid
    #130819
    Purpose
    Expresses mito-iFAST in mammalian cells (mitochondria labeling)
    Depositor
    Arnaud Gautier
    Insert
    mito-iFAST
    Use
    Tags
    myc-tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    130819-plasmid-map-sequence-id-254957

    Enlarge

  12. pCCG_nit9E

    Plasmid
    #228419
    Purpose
    Expression of NIT-9E domain in N. crassa at the his-3 locus
    Depositor
    Robert Haensch
    Insert
    NIT-9
    Use
    Protein expression in n. crassa
    Tags
    Expression
    Mutation
    truncation of the N terminal G domain and linkage…
    Promoter
    Available since
    Feb. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    228419-plasmid-map-sequence-id-460292

    Enlarge

  13. pCCG_nit9G

    Plasmid
    #228420
    Purpose
    Expression of NIT-9G domain in N. crassa at the his-3 locus
    Depositor
    Robert Haensch
    Insert
    NIT-9
    Use
    Protein expression in n. crassa
    Tags
    Expression
    Mutation
    truncation of the C terminal E domain and linkage…
    Promoter
    Available since
    Feb. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    228420-plasmid-map-sequence-id-460291

    Enlarge

  14. pCCG_nit9_A184-G203

    Plasmid
    #228421
    Purpose
    Expression of NIT-9 wit a 20 aa truncation in N. crassa at the his-3 locus
    Depositor
    Robert Haensch
    Insert
    NIT-9
    Use
    Protein expression in n. crassa
    Tags
    Expression
    Mutation
    truncation of A184-G203
    Promoter
    Available since
    Feb. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    228421-plasmid-map-sequence-id-460290

    Enlarge

  15. pCCG_nit9_G204-G223

    Plasmid
    #228422
    Purpose
    Expression of NIT-9 wit a 20 aa truncation in N. crassa at the his-3 locus
    Depositor
    Robert Haensch
    Insert
    NIT-9
    Use
    Protein expression in n. crassa
    Tags
    Expression
    Mutation
    truncation of G204-G223
    Promoter
    Available since
    Feb. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    228422-plasmid-map-sequence-id-460288

    Enlarge

  16. pCCG_nit9_E224-G243

    Plasmid
    #228423
    Purpose
    Expression of NIT-9 wit a 20 aa truncation in N. crassa at the his-3 locus
    Depositor
    Robert Haensch
    Insert
    NIT-9
    Use
    Protein expression in n. crassa
    Tags
    Expression
    Mutation
    truncation of E224-G243
    Promoter
    Available since
    Feb. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    228423-plasmid-map-sequence-id-460277

    Enlarge

  17. pCCG_nit9_K264-P282

    Plasmid
    #228425
    Purpose
    Expression of NIT-9 wit a 20 aa truncation in N. crassa at the his-3 locus
    Depositor
    Robert Haensch
    Insert
    NIT-9
    Use
    Protein expression in n. crassa
    Tags
    Expression
    Mutation
    truncation of K264-P282
    Promoter
    Available since
    Feb. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    228425-plasmid-map-sequence-id-460271

    Enlarge

  18. pCCG_nit9G_linker

    Plasmid
    #228413
    Purpose
    Expression of NIT-9G with linkage region at the C terminus in N. crassa at the his-3 locus
    Depositor
    Robert Haensch
    Insert
    NIT-9
    Use
    Protein expression in n. crassa
    Tags
    Expression
    Mutation
    truncation of the C terminal E domain
    Promoter
    Available since
    Jan. 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    228413-plasmid-map-sequence-id-459453

    Enlarge

  19. pCCG_linker_nit9E

    Plasmid
    #228412
    Purpose
    Expression of NIT-9E with linkage region at the N terminus in N. crassa at the his-3 locus
    Depositor
    Robert Haensch
    Insert
    NIT-9
    Use
    Protein expression in n. crassa
    Tags
    Expression
    Mutation
    truncation of the N terminal G domain
    Promoter
    Available since
    Jan. 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    228412-plasmid-map-sequence-id-459452

    Enlarge

  20. UCT1m

    Plasmid
    #121041
    Purpose
    MoClo golden gate assembly DE part for gQi gRNA (guide RNA for S. pyogenes Cas9; sequence from DOI: 10.1016/j.cell.2013.02.022). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    UCT-part guide RNA (Stanley Qi sequence)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121041-plasmid-map-sequence-id-245418

    Enlarge

Showing: 121 - 140 of 12614 results