We narrowed to 13,016 results for: SHI

1. pCMV-GenEPi

   Plasmid

   #140236

   Purpose

   Mammalian expression of Piezo1-based fluorescent force sensor GenEPi

   Depositor

   Periklis Pantazis

   Article

   Yaganoglu et al Nat Commun. 2023 Jul 19;14(1):4352. doi:

   Insert

   GenEPi (Piezo1-based fluorescent force sensor) (PIEZO1 Human)
   

   Use

   Tags

   GCaMP 6s RS1 EF4

   Expression

   Mammalian

   Mutation

   Promoter

   simian CMV IE94

   Available since

   July 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. lentiCRISPR - EGFP sgRNA 1

   Plasmid

   #51760

   Purpose

   Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

   Depositor

   Feng Zhang

   Article

   Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:

   Inserts

   Cas9
   Puromycin resistance
   EGFP sgRNA 1
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EFS and U6

   Available since

   March 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV-SARS-CoV-2 5'UTR-Luciferase

   Plasmid

   #191480

   Purpose

   Luciferase reporter for SARS-CoV-2 5' UTR

   Depositor

   Hao Wu

   Article

   Vora et al Proc Natl Acad Sci U S A. 2022 Mar 1;119

   Insert

   SARS-CoV-2 5' UTR
   

   Use

   Lentiviral and Luciferase

   Tags

   Expression

   Mutation

   contains SARS-CoV-2 5' UTR

   Promoter

   Available since

   Nov. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGD-P1/HC-Pro

   Plasmid

   #196328

   Purpose

   35S promoter-driven expression of the tobacco etch virus P1/HC-Pro RNA silencing suppressor

   Depositor

   Zhenghe Li

   Article

   Ganesan et al J Virol. 2013 Oct;87(19):10598-611. doi:

   Insert

   tobacco etch virus P1/HC-Pro
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   duplicated cauliflower mosaic virus 35S promoter

   Available since

   May 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGD-γb

   Plasmid

   #196327

   Purpose

   35S promoter-driven expression of the barley stripe mosaic virus γb RNA silencing suppressor

   Depositor

   Zhenghe Li

   Article

   Ganesan et al J Virol. 2013 Oct;87(19):10598-611. doi:

   Insert

   barley stripe mosaic virus γb
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   duplicated cauliflower mosaic virus 35S promoter

   Available since

   May 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. GBX

   Plasmid

   #64123

   Purpose

   a modified episomal (EBNA1/OriP) vector expressing human eGFP and BCL-xL genes

   Depositor

   Linzhao Cheng

   Article

   Chou et al Stem Cells Transl Med. 2015 Mar 5. pii:

   Insert

   GFP-2A-BCL2L1 (BCL2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   Aug. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-FNLS-P2A-Puro

   Plasmid

   #110841

   Purpose

   Lentiviral vector for constitutive expression of FNLS in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   BE3RA-FNLS
   

   Use

   Lentiviral

   Tags

   3X FLAG

   Expression

   Mutation

   D10A and NLS sequence at the N-terminus

   Promoter

   EF1s

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. Pet22b-mEGFP

   Plasmid

   #166439

   Purpose

   Bacterial expression of 6x His tagged mEGFP

   Depositor

   Shuguo Sun

   Article

   Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

   Insert

   mEGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFastBac1-His10FLAG

   Plasmid

   #157736

   Purpose

   empty pFastBac1 vector (polyhedrin promoter) adds enterokinase-removable N-terminal His10-tag + 3xFLAG tag; avoids unwanted residues in the final protein sequence by using the BseRI restriction sites

   Depositor

   Debra Hansen

   Article

   Olson et al Protein Expr Purif. 2021 May 7:105890. d

   Type

   Empty backbone

   Use

   Tags

   His10 + 3xFLAG, enterokinase-cleavable

   Expression

   Insect

   Mutation

   Promoter

   polyhedrin

   Available since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAV_Nanog HMEJ donor

    Plasmid

    #97321

    Purpose

    HMEJ donor for fusing a p2A-mCherry reporter to mouse Nanog. AAV backbone.

    Depositor

    Hui Yang

    Article

    Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

    Insert

    Nanog HMEJ donor
    

    Use

    AAV and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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