-
-
6BHD (SETDB1)
Plasmid#119955PurposeBacterial expression for structure determinationDepositorInsertSETDB1 (SETDB1 Human)
UseTags6xHis-TEVExpressionBacterialMutationcontains amino acids 190-410PromoterT7Available sinceFeb. 1, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
UIMLx2
Plasmid#174888PurposeUIMLx2 ubiquitin probe for K48 ubiquitylated proteinsDepositorInsertMet4 UIML
UseTagsAvi-6xHis-Smt3ExpressionBacterialMutationPromoterAvailable sinceNov. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
CXXC1
Plasmid#32045PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertCXXC1 (CXXC1 Human)
UseTagsHis (with TEV cleavage)ExpressionBacterialMutationCXXC domain (amino acids residues 161-222)PromoterAvailable sinceJan. 3, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
HDGF2
Plasmid#32021PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertHDGF2 (HDGFL2 Human)
UseTagsHisExpressionBacterialMutationPWWP domain only (amino acid residues 1-93)PromoterT7Available sinceJan. 3, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
5KCH (SETDB1)
Plasmid#101881PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertSETDB1 (SETDB1 Human)
UseTags6xHis, TEVExpressionBacterialMutationPromoterT7Available sinceApril 30, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pQuantFLAG-bsr-kana
Plasmid#45593DepositorTypeEmpty backboneUseCre/Lox; Dt40 cell line targetingTagsQuant-FLAG tagExpressionMutationPromoterAvailable sinceJuly 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pQuantFLAG-puro-kana
Plasmid#45592DepositorTypeEmpty backboneUseCre/Lox; Dt40 cell line targetingTagsQuant-FLAG tagExpressionMutationPromoterAvailable sinceJuly 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pQuantFLAG-hygro-kana
Plasmid#45591DepositorTypeEmpty backboneUseCre/Lox; Dt40 cell line targetingTagsQuant-FLAG tagExpressionMutationPromoterAvailable sinceJuly 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pQuantEGFP-bsr-kana
Plasmid#45590DepositorTypeEmpty backboneUseCre/Lox; Dt40 cell line targetingTagsQuant-EGFP tagExpressionMutationPromoterAvailable sinceJuly 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pQuantEGFP-puro-kana
Plasmid#45589DepositorTypeEmpty backboneUseCre/Lox; Dt40 cell line targetingTagsQuant-EGFP tagExpressionMutationPromoterAvailable sinceJuly 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
Kif1A-EGFP-SNAP
Plasmid#229851PurposeKif1A (aa1-351)-Kif1A neck linker(17aa) fused to Kin1 coiled coil (aa345-406)-EGFP-SNAP-his. This Kif1A was used to link an oligo to the motor using the SNAP tag for single molecule experimentsDepositorInsertKif1A-EGFP-SNAP (Kif1a Rat)
UseTags6xHis, EGFP, and SNAP-tagExpressionBacterialMutationPromoterAvailable sinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+)-T7-OmpA-MNaseA-6xHis
Plasmid#214808PurposeExpression of 6xhis-tagged micrococcal nuclease A (MNaseA)DepositorInsertOmpA-Micrococcal nuclease A
UseTags6xhisExpressionBacterialMutationPromoterT7 promoterAvailable sinceNov. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+)-T7-OmpA-MNaseB-6xHis
Plasmid#214809PurposeExpression of 6xhis-tagged micrococcal nuclease B (MNaseB)DepositorInsertOmpA-Micrococcal nuclease B
UseTags6xhisExpressionBacterialMutationPromoterT7 promoterAvailable sinceNov. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
TCT-Universal plasmid
Plasmid#221852PurposeA universal plasmid for expressing any protein with TetraCysteine motifDepositorInsertTetra Cysteine tag
UseTagsExpressionBacterialMutationNoPromoterAvailable sinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
SARS-CoV-2 Mpro protease (T190I mutant - clinical mutant); aliases: 3CLpro, 3C-like
Plasmid#204786PurposeBacterial expression of Sars Cov 2 MPRO T190I mutantDepositorInsertSars Cov 2 MPRO (ORF1ab Sars Cov 2)
UseTagsExpressionBacterialMutationT190IPromoterAvailable sinceJan. 23, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
SARS-CoV-2 Mpro protease (Q189K mutant - clinical mutant); aliases: 3CLpro, 3C-like
Plasmid#204784PurposeBacterial expression of Sars Cov 2 MPRO Q189K mutantDepositorInsertSars Cov 2 MPRO (ORF1ab Sars Cov 2)
UseTagsExpressionBacterialMutationQ189KPromoterAvailable sinceJan. 23, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
SARS-CoV-2 Mpro protease (P132H mutant - clinical mutant); aliases: 3CLpro, 3C-like
Plasmid#204783PurposeBacterial expression of Sars Cov 2 MPRO P132H mutantDepositorInsertSars Cov 2 MPRO (ORF1ab Sars Cov 2)
UseTagsExpressionBacterialMutationP132HPromoterAvailable sinceJan. 23, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
SARS-CoV-2 Mpro protease (N142S mutant - clinical mutant); aliases: 3CLpro, 3C-like
Plasmid#204782PurposeBacterial expression of Sars Cov 2 MPRO N142S mutantDepositorInsertSars Cov 2 MPRO (ORF1ab Sars Cov 2)
UseTagsExpressionBacterialMutationN142SPromoterAvailable sinceJan. 23, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
SARS-CoV-2 Mpro protease (M49T mutant - clinical mutant); aliases: 3CLpro, 3C-like
Plasmid#204779PurposeBacterial expression of Sars Cov 2 MPRO M49T mutantDepositorInsertSars Cov 2 MPRO (ORF1ab Sars Cov 2)
UseTagsExpressionBacterialMutationM49TPromoterAvailable sinceJan. 23, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits
