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pLenti_EF1a_EV

Plasmid

#183116

Purpose

Empty vector control for pLenti_EF1a constructs

Depositor

William Kaelin

Article

Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

Insert

Small multiple cloning site

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_SBP-EGFP-Ecadherin

Plasmid

#65286

Purpose

synchronize trafficking of Ecadherin from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Ecadherin

Tags

EGFP and IL-2 Signal Sequence and Streptavidin Bi…

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3 FLAG RRM2

Plasmid

#236465

Purpose

transient overexpression of RRM2 in mammalian cells

Depositor

Michele Pagano

Article

D'Angiolella et al Cell. 2012 May 25;149(5):1023-34. doi: 1

Insert

RRM2 (RRM2 Human)

Expression

Mammalian

Available Since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti_EF1a_Flag_EGFP

Plasmid

#183117

Purpose

Expresses flag-tagged EGFP in mammalian cells

Depositor

William Kaelin

Article

Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

Insert

EGFP

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a-HaloTag-CNA35

Plasmid

#215457

Purpose

Encodes the bacterial collagen-binding protein CNA35 with N-terminal HaloTag.

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

CNA35

Tags

HaloTag

Expression

Bacterial

Mutation

Includes stop codon

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pLentiCRISPR_Puro_sgTP53

Plasmid

#187819

Purpose

pLentiCRISPR that is selectable with puromycin with an sgRNA targeting TP53

Depositor

William Kaelin

Article

Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

Insert

sgTP53

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_TYMS

Plasmid

#183326

Purpose

All-in-One CRISPRko system with a guide RNA that targets TYMS gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

TYMS

Use

Lentiviral

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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AChE-pcDNA

Plasmid

#106442

Purpose

Mammalian expression of murine acetylcholinesterase

Depositor

Georg Weber

Article

Syed et al Clin Exp Metastasis. 2008;25(7):787-98.

Insert

acetylcholinesterase (ACHE Human)

Expression

Mammalian

Mutation

WT

Available Since

April 4, 2018

Availability

Academic Institutions and Nonprofits only

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pHAGE2_EF1a_TetOn_IRES_mCherry

Plasmid

#204155

Purpose

Lentivirus backbone expressing rtTA linked to mCherry

Depositor

Jesse Bloom

Article

Dadonaite et al Cell. 2023 Mar 16;186(6):1263-1278.e20.

Insert

Tet-On 3G

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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HDM-Hgpm2

Plasmid

#204152

Purpose

Lentivirus helper plasmid coding for Gag/Pol

Depositor

Jesse Bloom

Article

Dadonaite et al Cell. 2023 Mar 16;186(6):1263-1278.e20.

Insert

HIV-1 gag/pol

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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