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pCK948

Plasmid

#242917

Purpose

Constitutive expression of dCas9, MCP-TetD, and PspF-λN22

Depositor

Jesse Zalatan

Article

Kiattisewee et al ACS Synth Biol. 2025 Jul 22. doi: 10.102

Insert

PspF-λN22 (pspF Synthetic)

Use

CRISPR and Synthetic Biology

Promoter

BBa_J23107

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pCK660

Plasmid

#242914

Purpose

Constitutive expression of dCas9, MCP-SoxS, and PspF-λN22

Depositor

Jesse Zalatan

Article

Kiattisewee et al ACS Synth Biol. 2025 Jul 22. doi: 10.102

Insert

PspF-λN22 (pspF Synthetic)

Use

CRISPR and Synthetic Biology

Promoter

BBa_J23107

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pLJC6-NADK2(delta MTS)-3xFLAG

Plasmid

#233896

Purpose

lentiviral overexpression vector for NADK2 (delta MTS)

Depositor

Jason Cantor

Article

Flickinger et al Nat Metab. 2025 May 2. doi: 10.1038/s422

Insert

NADK2 (NADK2 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

delta MTS (see Depositor Comments)

Promoter

Ubc

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-v1-sgNADK2

Plasmid

#233897

Purpose

lentiviral vector expressing Cas9 and an sgRNA targeting NADK2

Depositor

Jason Cantor

Article

Flickinger et al Nat Metab. 2025 May 2. doi: 10.1038/s422

Insert

sgRNA targeting NADK2 (NADK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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Reckleen_3plus_sgRNA_ptet_pos4

Plasmid

#233459

Purpose

Level 1 part, Ptet sfgfp dropout sgRNA for position 4, for building RECKLEEN plasmid with multiple sgRNAs

Depositor

Anke Becker

Article

Elsayed et al Commun Biol. 2025 Oct 30;8(1):1509. doi:

Insert

Level 1 part, Ptet sfgfp dropout sgRNA for position 4, ColE1 ori, and KanR.

Use

CRISPR

Available Since

April 24, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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Reckleen_3plus_sgRNA_ptet_pos5

Plasmid

#233460

Purpose

Level 1 part, Ptet sfgfp dropout sgRNA for position 5, for building RECKLEEN plasmid with multiple sgRNAs

Depositor

Anke Becker

Article

Elsayed et al Commun Biol. 2025 Oct 30;8(1):1509. doi:

Insert

Level 1 part, Ptet sfgfp dropout sgRNA for position 5, ColE1 ori, and KanR.

Use

CRISPR

Available Since

April 24, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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Reckleen_3plus_sgRNA_ptet_pos5a

Plasmid

#233461

Purpose

Level 1 part, Ptet sfgfp dropout sgRNA for position 5a, for building RECKLEEN plasmid with multiple sgRNAs

Depositor

Anke Becker

Article

Elsayed et al Commun Biol. 2025 Oct 30;8(1):1509. doi:

Insert

Level 1 part, Ptet sfgfp dropout sgRNA for position 5a, ColE1 ori, and KanR.

Use

CRISPR

Available Since

April 24, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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Reckleen_3plus_sgRNA_ptet_pos5a1

Plasmid

#233462

Purpose

Level 1 part, Ptet sfgfp dropout sgRNA for position 5a1, for building RECKLEEN plasmid with multiple sgRNAs

Depositor

Anke Becker

Article

Elsayed et al Commun Biol. 2025 Oct 30;8(1):1509. doi:

Insert

Level 1 part, Ptet sfgfp dropout sgRNA for position 5a1, ColE1 ori, and KanR.

Use

CRISPR

Available Since

April 24, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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Reckleen_3plus_sgRNA_ptet_pos5a2

Plasmid

#233463

Purpose

Level 1 part, Ptet sfgfp dropout sgRNA for position 5a2, for building RECKLEEN plasmid with multiple sgRNAs

Depositor

Anke Becker

Article

Elsayed et al Commun Biol. 2025 Oct 30;8(1):1509. doi:

Insert

Level 1 part, Ptet sfgfp dropout sgRNA for position 5a2, ColE1 ori, and KanR.

Use

CRISPR

Available Since

April 24, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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Reckleen_3plus_sgRNA_ptet_pos5b

Plasmid

#233464

Purpose

Level 1 part, Ptet sfgfp dropout sgRNA for position 5b, for building RECKLEEN plasmid with multiple sgRNAs

Depositor

Anke Becker

Article

Elsayed et al Commun Biol. 2025 Oct 30;8(1):1509. doi:

Insert

Level 1 part, Ptet sfgfp dropout sgRNA for position 5b, ColE1 ori, and KanR.

Use

CRISPR

Available Since

April 24, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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Reckleen_3plus_sgRNA_ptet_pos5b1

Plasmid

#233465

Purpose

Level 1 part, Ptet sfgfp dropout sgRNA for position 5b1, for building RECKLEEN plasmid with multiple sgRNAs

Depositor

Anke Becker

Article

Elsayed et al Commun Biol. 2025 Oct 30;8(1):1509. doi:

Insert

Level 1 part, Ptet sfgfp dropout sgRNA for position 5b1, ColE1 ori, and KanR.

Use

CRISPR

Available Since

April 24, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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Reckleen_3plus_sgRNA_ptet_pos5b2

Plasmid

#233466

Purpose

Level 1 part, Ptet sfgfp dropout sgRNA for position 5b2, for building RECKLEEN plasmid with multiple sgRNAs

Depositor

Anke Becker

Article

Elsayed et al Commun Biol. 2025 Oct 30;8(1):1509. doi:

Insert

Level 1 part, Ptet sfgfp dropout sgRNA for position 5b2, ColE1 ori, and KanR.

Use

CRISPR

Available Since

April 24, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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Reckleen_1_Cas9

Plasmid

#233456

Purpose

Level 2 RECKLEEN plasmid, genome editing of Klebsiella pneumoniae

Depositor

Anke Becker

Article

Elsayed et al Commun Biol. 2025 Oct 30;8(1):1509. doi:

Insert

Level 2 RECKLEEN plasmid

Use

CRISPR

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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EGFP_EHMT1V09

Plasmid

#228813

Purpose

Expression of mouse EHMT1 with deletion of aa 1-31

Depositor

Shravanti Rampalli

Article

Biligiri et al PLOS Biology 22(11): e3002871.

Insert

EHMT1 (Ehmt1 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

deletion of aa 1-31

Available Since

March 10, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGGS-PHOSPHO1-TEV-Twin-Strep

Plasmid

#222015

Purpose

Express human PHOSPHO1 protein (C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-fusion protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK)) in mammalian cell

Depositor

Chiaki Murakami

Insert

PHOSPHO1 (PHOSPHO1, PHOSPHO1 Human)

Tags

TEV cleavable site and Twin-Strep-tag

Expression

Mammalian

Mutation

stop codon (TGA) is deleted

Promoter

chicken β-actin promoter

Available Since

Jan. 8, 2025

Availability

Academic Institutions and Nonprofits only

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MAC_N_COMMD1

Plasmid

#209674

Purpose

Expresses MAC-tagged COMMD1 in mammalian cells

Depositor

Markku Varjosalo

Article

Laulumaa et al Nat Struct Mol Biol. 2024 Jun;31(6):925-

Insert

COMMD1 (COMMD1 Human)

Tags

MAC-tag

Expression

Mammalian

Available Since

Aug. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pAc5.1-PH-mCherry-NOT2 isoform A (HK99)

Plasmid

#206456

Purpose

NOT2 expression in Schneider cells for imaging

Depositor

Mandy Jeske

Article

Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

Insert

NOT2 (Rga Fly)

Tags

PH mcherry

Expression

Insect

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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pMx/ATP11C-c-HA

Plasmid

#209239

Purpose

Mammalian expression of ATP11C isoform c

Depositor

Hye-Won Shin

Article

Inoue et al J Cell Sci. 2021 Oct 15;134(20):jcs25852

Insert

ATP11C isoform c (ATP11C Human)

Use

Retroviral

Tags

HA

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pMx/ATP11C-b-HA

Plasmid

#209238

Purpose

Mammalian expression of ATP11C isoform b

Depositor

Hye-Won Shin

Article

Inoue et al J Cell Sci. 2021 Oct 15;134(20):jcs25852

Insert

ATP11C isoform b (ATP11C Human)

Use

Retroviral

Tags

HA

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pMXSIP-CMTM6-HA

Plasmid

#204710

Purpose

expresses CMTM6-HA in mammalian cells

Depositor

William Kaelin

Article

Wyant et al Science. 2022 Aug 5;377(6606):621-629. d

Insert

CMTM6 (CMTM6 Human)

Tags

HA

Expression

Mammalian

Available Since

Sept. 21, 2023

Availability

Academic Institutions and Nonprofits only

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