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Plasmid#60740PurposeA modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'ACAT' between -11 and -8.DepositorInsertGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceNov. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET:CCCT:GFP
Plasmid#60738PurposeA modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'CCCT' between -11 and -8.DepositorInsertGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceDec. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
L3MBTL3 (3UT1)
Plasmid#36901PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertL3MBTL3 (L3MBTL3 Human)
UseTagsHis tag and Streptavidin-Binding Peptide (SBP)-Ta…ExpressionBacterialMutationcontains amino acid residues 228-551 onlyPromoterAvailable sinceJune 21, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
Kif3A-EGFP-SNAP
Plasmid#229852PurposeKif3A (aa1-342)-Kif1A neck linker(17aa) fused to Kin1 coiled coil (aa345-406)-EGFP-SNAP-his. This Kif3A was used to link an oligo to the motor using the SNAP tag for single molecule experimentsDepositorInsertKif3A (Kif3a Mouse)
UseTags6xHis, EGFP, and SNAP-tagExpressionBacterialMutationPromoterT7 promoterAvailable sinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
Sars Cov 2 MPRO R188S mutantSARS-CoV-2 Mpro protease (R188S mutant - clinical mutant); aliases: 3CLpro, 3C-like
Plasmid#204785PurposeBacterial expression of Sars Cov 2 MPRO R188S mutantDepositorInsertSars Cov 2 MPRO (ORF1ab Sars Cov 2)
UseTagsExpressionBacterialMutationR188SPromoterAvailable sinceJan. 23, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET-PF2050
Plasmid#100721PurposeExpression of Pyrococcus furiosus PF2050DepositorInsertPF2050
UseTagsExpressionBacterialMutationPromoterT7 promotorAvailable sinceOct. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET:CACT:GFP
Plasmid#60737PurposeA modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'CACT' between -11 and -8.DepositorInsertGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceNov. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
DMTF1
Plasmid#36895PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertDMTF1 (DMTF1 Human)
UseTagsHis tag with TEV cleavageExpressionBacterialMutationcontains Myb-like 1 domain, residues 220-274PromoterAvailable sinceJune 19, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
Ecoli PancACe
Plasmid#215712PurposeExpresses acetyl-CoA biosensor in EcoliDepositorInsertPancACe
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
T. aq RNA Polymerase
Plasmid#215349PurposeExpress T. aq RNA Polymerase subunits in E. coli cellsDepositorInsertRNA polymerase
UseTags6x HisExpressionBacterialMutationPromoterAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
T. aq sigmaA
Plasmid#215350PurposeExpress T. aq sigmaA transcription initiation factor in E. coli cellsDepositorInsertrpoD
UseTags6x HisExpressionBacterialMutationPromoterAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHis-hTG2
Plasmid#100719PurposeExpression of human transglutaminase 2 in E.coliDepositorInsertTGM2 (TGM2 Human)
UseTagsHisExpressionBacterialMutationsilent mutation Arg580 (AGG--CGT) to enhance expr…PromoterT7Available sinceNov. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHYP-NPC-10H
Plasmid#162789PurposeSARS-CoV-2 Nucleocapsid Protein expression in E.coliDepositorInsertNucleocapsid phosphoprotein [Severe acute respiratory syndrome coronavirus 2] (N Severe acute respiratory syndrome coronavirus 2)
UseTags10xHisExpressionBacterialMutationPromoterT7lacAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHis-hPim1
Plasmid#100722PurposeExpression of human Pim1 kinase domain in E.coliDepositorInsertPim1 (PIM1 Human)
UseTagsHisExpressionBacterialMutationResidues 29-313 of isoform 2PromoterT7 promotorAvailable sinceOct. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
HOXA9 (murine) FLAG pET
Plasmid#8494DepositorInsertHOXA9 (Hoxa9 Mouse)
UseTagsFLAG and HisExpressionBacterialMutationFLAG epitope tag replaces T7 tag in the pET back…PromoterAvailable sinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET 28 full-length human PARP1
Plasmid#192635PurposeExpresses human PARP1 with an N-terminal His-tagDepositorInsertHuman PARP1 (PARP1 Human)
UseTagshistidine-tagExpressionBacterialMutationPolymorphism A762PromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHis-hMTMR1
Plasmid#100723PurposeExpression of human MTMR1 PH-PTP domainDepositorInsertMTMR1 (MTMR1 Human)
UseTagsHisExpressionBacterialMutationContains amino acids 95-607PromoterT7Available sinceNov. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pAGM22082_cRed
Plasmid#117225PurposepET-28b backbone plasmid, Level 2 Golden Gate expression plasmid.DepositorInsertCanthaxanthin Operon
UseTags34 amino acid N-terminal linker, Hexahistidine Ta…ExpressionBacterialMutationremoved Type IIS restriction sites within the bac…PromoterT7 RNA PolymeraseAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZM3[I:FruR-L,I:RbsR-L]
Plasmid#60777PurposeContains PI driving expression FruR-L, and PI driving expression of RbsR-L.DepositorInsertsFruR-L
RbsR-L
UseTagsExpressionMutationLacI/GalR repressor chimera. LacI DBD with FruR L…PromoterAvailable sinceJuly 10, 2015AvailabilityAcademic Institutions and Nonprofits only