We narrowed to 14,424 results for: NTS

1. pUb FLAG-Mts H59Q

   Plasmid

   #208402

   Purpose

   Expresses FLAG-tagged Drosophila mts protein with H59Q mutation

   Depositor

   Jeremy Wilusz

   Article

   Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

   Insert

   microtubule star (mts Fly)
   

   Tags

   3xFLAG

   Expression

   Insect

   Mutation

   H59Q

   Promoter

   Ubi-p63e

   Available Since

   Dec. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUb FLAG-Mts H241A

   Plasmid

   #208404

   Purpose

   Expresses FLAG-tagged Drosophila mts protein with H241A mutation

   Depositor

   Jeremy Wilusz

   Article

   Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

   Insert

   microtubule star (mts Fly)
   

   Tags

   3xFLAG

   Expression

   Insect

   Mutation

   H241A

   Promoter

   Ubi-p63e

   Available Since

   Dec. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCX-DI-2A

   Plasmid

   #74671

   Purpose

   Mammalian expression of DI-2A (hCD73/F2A/hCD39)

   Depositor

   Roberto Giovannoni

   Article

   De Giorgi et al Nucleosides Nucleotides Nucleic Acids. 2

   Inserts

   CD73 (NT5E Human)
   CD39 (ENTPD1 Human)
   

   Tags

   F2A

   Expression

   Mammalian

   Promoter

   pCAGGS

   Available Since

   Feb. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. Lenti-sgNT.2#2/Cre

   Plasmid

   #173661

   Purpose

   Expresses a NT.2-targeting gRNA and Cre-recombinase

   Depositor

   Charles Swanton

   Article

   Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

   Insert

   Non-targeting gRNA
   

   Use

   CRISPR, Cre/Lox, and Lentiviral

   Available Since

   May 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEMHE-membrane-mEGFP

   Plasmid

   #105526

   Purpose

   T7 promotor drives in vitro mRNA transcription of a mEGFP-tagged membrane reporter

   Depositor

   Melina Schuh

   Article

   Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

   Insert

   GNAI2 (GNAI2 Human)
   

   Use

   In vitro transcription

   Tags

   mEGFP

   Mutation

   fragment encoding the plasma membrane targeting s…

   Promoter

   T7

   Available Since

   March 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEX-6p1-GST-ArcΔCTD

   Plasmid

   #119878

   Purpose

   Bacterial Expression construct for the production and purification of Arc missing C-terminal Domain

   Depositor

   Jason Shepherd

   Article

   Pastuzyn et al Cell. 2018 Mar 22;173(1):275. doi: 10.10

   Insert

   Activity Regulated Cytoskeletal Protein (Arc Rat)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Deletion of nt 832-1110

   Promoter

   tac

   Available Since

   March 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. hum alpha ENAC promoter a25-a23

   Plasmid

   #83427

   Purpose

   promoter reporter construct to test 5' flanking sequences

   Depositor

   Christie Thomas

   Article

   Sayegh et al J Biol Chem. 1999 Apr 30;274(18):12431-7

   Insert

   human aENaC gene 5' flanking seq + 55 nt of the 5' UTR for exon 1A (SCNN1A Human)
   

   Use

   Luciferase

   Tags

   Luciferase

   Promoter

   alpha ENAC

   Available Since

   April 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. Lenti-sgNT.3#3/Cre

   Plasmid

   #173662

   Purpose

   Expresses a NT.3-targeting gRNA and Cre-recombinase

   Depositor

   Charles Swanton

   Article

   Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

   Insert

   Non-targeting gRNA
   

   Use

   CRISPR, Cre/Lox, and Lentiviral

   Available Since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLKO.1-puro U6 sgRNA BfuAI stuffer A-tract

   Plasmid

   #138525

   Purpose

   Expresses a guide RNA of choice cloned into the BfuI site extended at the 3' end to incorporate a 220-nt control A-tract repeat RNA sequence.

   Depositor

   Richard Jenner

   Article

   Beltran et al Nat Struct Mol Biol. 2019 Oct;26(10):899

   Insert

   Control non-PRC2 binding A-tract repeat RNA
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Aug. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDF-casBCDE(g8)

    Plasmid

    #89727

    Purpose

    Expresses the Cascade subunits Cse2, Cas7, Cas5, Cas6e and pre-crRNA containing 33-nt spacer (referred to as 'wt') matching a protospacer in the M13 phage genome.

    Depositor

    Konstantin Kuznedelov, Konstantin Severinov

    Article

    Kuznedelov et al Nucleic Acids Res. 2016 Dec 15;44(22):10

    Insert

    CRISPR array
    

    Expression

    Bacterial

    Available Since

    May 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1_SNORD27h_2ntmut

    Plasmid

    #73067

    Purpose

    expression clone for human mutated SNORD27 (C/D box snoRNA U27)

    Depositor

    Stefan Stamm

    Article

    Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

    Insert

    SNORD27 (E2F7 Human)
    

    Tags

    no tag

    Expression

    Mammalian

    Mutation

    2 nt mutation in AS box that binds E2F7 pre-mRNA

    Available Since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

12. pMCh2008_pLenti_Syn_mChe-cdc42E7-E63'UTR

    Plasmid

    #118622

    Purpose

    expression of mChe-tagged cdc42E7-E6-3'UTR under pSyn promoter for primary neurons

    Depositor

    Marina Chekulaeva

    Article

    Ciolli Mattioli et al Nucleic Acids Res. 2018 Dec 22. pii: 525

    Insert

    cdc42E7-E6 3'UTR (Cdc42 Mouse)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    changed ttgcttt to cggtaag (496 - 502 nt in 3…

    Promoter

    synapsin I (rat)

    Available Since

    March 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMEXneo-TrkC-Δ6

    Plasmid

    #190204

    Purpose

    To express the human TrkC that has in the extracellular domain only the second IgG like domain. Still binds NT-3.

    Depositor

    Pantelis Tsoulfas

    Article

    Urfer et al J Biol Chem. 1998 Mar 6;273(10):5829-40.

    Insert

    TrkC (NTRK3 Human)
    

    Expression

    Mammalian

    Mutation

    The extracellualr domain conatins only the signal…

    Promoter

    Moloney mouse sarcoma virus right LTR

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUb FLAG-tws

    Plasmid

    #195068

    Purpose

    Expresses FLAG-tagged Drosophila tws protein

    Depositor

    Jeremy Wilusz

    Article

    Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

    Insert

    twins (tws Fly)
    

    Tags

    3xFLAG

    Expression

    Insect

    Promoter

    Ubi-p63e

    Available Since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRosettav2

    Plasmid

    #115557

    Purpose

    To determine correct antibiotic selection conditions in mammalian cells; standard for titering experiments

    Depositor

    John Doench

    Insert

    Hygromycin-Blasticidin-Puromycin-EGFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Oct. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti-ePEG-LINE1-loxPsym

    Plasmid

    #216866

    Purpose

    Lentiviral guide RNA vector to insert the loxPsym sequence into LINE1 elements

    Depositor

    Leopold Parts

    Insert

    spacerLINE1_cr772scaffold_PBS-loxPsm-HA-tevopreQ
    

    Use

    CRISPR and Lentiviral

    Promoter

    U6

    Available Since

    Aug. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRSET.HaloTag.v7.CoOp.Cys-less

    Plasmid

    #195487

    Purpose

    Bacterial expression plasmid with HaloTag v7 insert; modified to replace cysteines C61, C262, and optimized to human codon bias/preference for use in oxidizing environments.

    Depositor

    Tim Brown, HHMI-JRC Tool Translation Team

    Insert

    HaloTag.v7
    

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    C61T, C262V

    Promoter

    T7

    Available Since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDR195

    Plasmid

    #36028

    Depositor

    Wolf Frommer, Dominique Loque

    Article

    Rentsch et al FEBS Lett. 1995 Aug 21;370(3):264-8.

    Type

    Empty backbone

    Expression

    Yeast

    Promoter

    pPMA1

    Available Since

    July 26, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDR196

    Plasmid

    #36029

    Depositor

    Wolf Frommer, Dominique Loque, Doris Rentsch

    Article

    Meyer et al J Biol Chem. 2006 Mar 17;281(11):7197-20

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    June 21, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGTEVDNIb

    Plasmid

    #122530

    Purpose

    Binary plasmid with a TEV (NIb deleted) clone

    Depositor

    Jose-Antonio Daros

    Article

    Daros et al Methods Mol Biol. 2020;2083:303-312. doi

    Insert

    Tobacco etch virus (NIb deleted) cDNA
    

    Use

    Potyvirus vectors to produce carotenoids in plants

    Available Since

    May 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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