We narrowed to 24,865 results for: Nes
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Plasmid#229475PurposeExpresses RssB with E135A, E136A, & E137A mutationDepositorInsertRssB (E135A, E136A, E137A)
ExpressionBacterialMutationE135A, E136A, E137APromoterT7Available SinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMS21
Plasmid#229476PurposeExpresses RssB with E135A, E136A, & E137A mutation with His-tagDepositorInsertRssB (E135A, E136A, E137A)
Tags10xHisExpressionBacterialMutationE135A, E136A, E137APromoterT7Available SinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVF18
Plasmid#229456PurposeExpresses IraD (aa17-130) with His-tagDepositorInsertIraD (aa 17-130 only)
Tags6xHisExpressionBacterialMutationaa 17-130 onlyPromoterT7Available SinceJan. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
P302-FBP_1
Plasmid#139692PurposeT-DNA encoding Fungal Bioluminescent Pathway Components, complete pathway, without recycling gene CPHDepositorInsertp35S_Short_TMV 5':AsNpga:tAtug7, AtUbi10:NnH3H:AtuOCS, pCmYLCV:NnHisps:tHsp, pAtuMas:NnLuz:trbcSE9
UseLuciferaseExpressionPlantPromotervariousAvailable SinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
P313-FBP_8
Plasmid#139699PurposeT-DNA encoding Fungal Bioluminescent Pathway Components, control pathway, missing H3HDepositorInsertpLHB1B1:NnCPH:tSlH4, p35S_Short_TMV 5':AsNpga:tAtug7, pCmYLCV:NnH3H:AtuOcs, pCmYLCV:NnHisps:tHsp, pAtuMas:Nn Luz:trbcSE9
UseLuciferaseExpressionPlantPromotervariousAvailable SinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
EGFR-mEGFP
Plasmid#203774PurposeNumber and brightness analysis of EGFR oligomerizationDepositorAvailable SinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
p16 nlsGPS2
Plasmid#228093PurposeExpression plasmid for genetically-encoded FRET biosensor for Gibberellin (GA)DepositorInsertnlsGPS2
Tags6xHis-T7 XpressTag and cMycExpressionPlantPromoterp16Available SinceApril 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
P445-FBP_17
Plasmid#139708PurposeT-DNA encoding Fungal Bioluminescent Pathway Components, complete pathway, Luz promoter is flower specific, circadian controlledDepositorInsertpSlH4:NnCPH:tSlH4, pSlRBCS2:AsNpga:tAtuG7, pAtuMas:NnH3H:tAtuMas, p35S:NnHisps:t35S, pPhODO1:NnLuz:tAtuOcs
UseLuciferaseExpressionPlantPromotervariousAvailable SinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pXL-BacII-CAG-loxP-STOP-loxP-Venus
Plasmid#170520PurposePlasmid containing piggyBac ITRs flanking CAG, a loxP-STOP-loxP cassette and Venus (also see doi: 10.7554/eLife.63907 )DepositorInsertCAG-loxP-STOP-loxP-Venus
ExpressionMammalianPromoterCAGAvailable SinceFeb. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJAK184.tetR.PT5-3.dCas9
Plasmid#232482Purposeoptimized ahTet inducible dCas9 for insertion into C. difficile genomeDepositorInsertCatalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
UseCRISPRExpressionBacterialPromoterTetR-PT5-3Available SinceApril 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28a-MBP-CBX1-eReader
Plasmid#135089PurposeBacterial expression of engineered CBX1 with enhanced binding to H3K9meDepositorInsertCBX1 chromobox protein 1, engineered
Tags6xhis-tag and MBPExpressionBacterialMutationlysine 43 mutated to alanine; aspartic acid 59 mu…PromoterT7Available SinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
P447-FBP_19
Plasmid#139710PurposeT-DNA encoding Fungal Bioluminescent Pathway Components, complete pathway, Luz promoter is ABA responsiveDepositorInsertpSlH4:NnCPH:tSlH4, pSlRBCS2:AsNpga:tAtuG7, pAtuMas:NnH3H:tAtuMas, p35S:NnHisps:t35S, pAtRAB18:NnLuz:tAtuOcs
UseLuciferaseExpressionPlantPromotervariousAvailable SinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR-v2-Blast-Puro
Plasmid#167186PurposeLentiviral expression of sgRNA with blasticidin and puromycin resistance genesDepositorInsertBlasticidin S deaminase
UseCRISPR and LentiviralExpressionMammalianAvailable SinceOct. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-MBP-(CBX1)3-eReader
Plasmid#135090PurposeBacterial expression of engineered CBX1 fusions with enhanced binding to H3K9meDepositorInsertCBX1 chromobox protein 1, engineered x3
Tags6xhis-tag and MBPExpressionBacterialMutationlysine 43 mutated to alanine; aspartic acid 59 mu…PromoterT7Available SinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJT10R
Plasmid#98307PurposeOverexpressing yeast farnesyl pyrophosphate synthase Erg20p mutant N127W and Actinidia chinensis nerolidol/linalool synhtase AcNES1 under the control of GAL1/GAL2 promoterDepositorInsertTRPL3AcNES1>TRPL41B
ExpressionYeastMutationERG20 N127WAvailable SinceFeb. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJT2
Plasmid#98306PurposeOverexpressing yeast farnesyl pyrophosphate synthase Erg20p mutant N127W and Actinidia chinensis nerolidol/linalool synhtase AcNES1 under the control of TEF1/TEF2 promoterDepositorInsertPTEF2>ScERG20N127W>TRPL3>PTEF1>AcNES1>TRPL41B
ExpressionYeastMutationERG20 N127WAvailable SinceFeb. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLNCX2 ER:ras
Plasmid#67844Purpose4-hydroxytamoxifen inducible ER:ras fusion proteinDepositorInsertER1a H-RasG12V fusion
ExpressionMammalianMutationG525R renders the HBD largely insensitive to 17ß‐…PromoterCMVAvailable SinceAug. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYL156 (TRV RNA2)
Plasmid#148969PurposeModified Tobacco Rattle Virus RNA 2; used for virus induced gene silencing in plants along with pYL192 (TRV RNA1)DepositorInsertModified TRV RNA2
UseT-dna vectorMutationsee comments section belowAvailable SinceJune 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
FUCas9Cherry
Plasmid#70182PurposeExpresses Cas9 with fluorescent Cherry reporterDepositorInserthumanised S.pyogenes cas9
UseCRISPR and LentiviralTags3xFLAGExpressionMammalianPromoterhUbCAvailable SinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBabe HA-Runx1-neo
Plasmid#45815DepositorAvailable SinceJune 17, 2013AvailabilityAcademic Institutions and Nonprofits only