We narrowed to 16,109 results for: form

1. pSP64T3 BMP2 pro Vg1 (DM#119)

   Plasmid

   #15071

   Depositor

   Douglas Melton

   Article

   Thomsen et al Cell. 1993 Aug 13. 74(3):433-41.

   Insert

   BMP2-Vg1
   

   Use

   Sp6 expression for use in xenopus

   Available Since

   Oct. 18, 2007

   Availability

   Academic Institutions and Nonprofits only

2. pcDNA3.1-ARL16-His6

   Plasmid

   #216484

   Purpose

   Used for exogenous expression of human ARL16, short form, in mammalian cells. The encoded protein contains a C-terminal, glycine rich linker and His6 tag

   Depositor

   Richard Kahn

   Insert

   ADP-ribosylation factor like 16 (ARL16 Human)
   

   Tags

   includes GSGSGSHHHHHH (His6) tag at C-terminus

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. peGFP NOVA2 op

   Plasmid

   #224354

   Purpose

   Express GFP-tagged human NOVA2 (codon optimized)

   Depositor

   Nicolas Charlet-Berguerand

   Article

   Mattioli et al Am J Hum Genet. 2020 Apr 2;106(4):438-45

   Insert

   NOVA2 (NOVA2 Human)
   

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   codon optimized for expression in human cells

   Promoter

   CMV and T7

   Available Since

   Sept. 10, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pcDNA3.1+HIF1a-AS1_deltaExon1 (nucleotides 26-78)

   Plasmid

   #194175

   Purpose

   Expresses lncRNA HIF1a-AS1 mutant

   Depositor

   Matthias Leisegang, Ralf Brandes

   Article

   Leisegang et al Nat Commun. 2022 Nov 2;13(1):6563. doi:

   Insert

   HIF1a-AS1 (HIF1A-AS1 Human)
   

   Expression

   Mammalian

   Mutation

   Within Exon1, nucleotides 26-78 were removed

   Promoter

   CMV

   Available Since

   Jan. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV6-MPP8-delta55-118-10xHis

   Plasmid

   #194180

   Purpose

   Expresses MPP8-delta55-118_10xHis

   Depositor

   Matthias Leisegang, Ralf Brandes

   Article

   Leisegang et al Nat Commun. 2022 Nov 2;13(1):6563. doi:

   Insert

   MPHOSPH8 (MPHOSPH8 Human)
   

   Tags

   10xHis

   Expression

   Mammalian

   Mutation

   MPP8-delta55-118 (chromo domain deletion)

   Promoter

   CMV

   Available Since

   Jan. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-ERT2CreERT2

   Plasmid

   #13777

   Purpose

   Mammalian expression of conditionally active form of Cre recombinase (activated in response to tamoxifen)

   Depositor

   Connie Cepko

   Article

   Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

   Insert

   ERT2-Cre-ERT2 fusion protein
   

   Use

   Cre/Lox

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   April 20, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-CreERT2

   Plasmid

   #14797

   Purpose

   Mammalian expression of conditionally active form of Cre recombinase (not as tightly regulated as pCAG-ERT2CreERT2)

   Depositor

   Connie Cepko

   Article

   Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

   Insert

   Cre-ERT2 fusion protein
   

   Use

   Cre/Lox

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   April 20, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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8. HA-ISceID44A

   Plasmid

   #59424

   Purpose

   Expresses a catalytically inactive form of the endonuclease ISceI

   Depositor

   Tom Misteli

   Article

   Roukos et al Science. 2013 Aug 9;341(6146):660-4. doi

   Insert

   endonuclease ISceI mutant (I-SceI )
   

   Tags

   HA tag

   Expression

   Mammalian

   Mutation

   changed aspartate 44 to alanine (D44A)

   Available Since

   Dec. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. mScarlet-Rab9aS21N

   Plasmid

   #169073

   Purpose

   Express human dominant negative form of Rab9a(S21N) in mammalian cells

   Depositor

   Gia Voeltz

   Article

   Wu et al Dev Cell. 2021 Jan 11;56(1):52-66.e7. do

   Insert

   Rab9a (RAB9A Human)
   

   Tags

   mScarlet

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSANG10-3F

    Plasmid

    #39264

    Depositor

    John McCafferty

    Article

    Martin et al BMC Biotechnol. 2006 Dec 7;6:46.

    Type

    Empty backbone

    Use

    Bacterial expression, bacterial expression of re…

    Tags

    6xHis, pelB leader, and tri-FLAG

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDAI-SceI

    Plasmid

    #32061

    Purpose

    Construction of unmarked gene deletions. Broad host range replicative plasmid expressing the I-SceI homing endonuclease

    Depositor

    Miguel Valvano

    Article

    Flannagan et al Environ Microbiol. 2008 Jun;10(6):1652-6

    Insert

    I-SceI nuclease
    

    Expression

    Bacterial

    Promoter

    Pdhfr

    Available Since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLV-SFFV-ZFYVE27-WPRE-UbC-mCherry

    Plasmid

    #214470

    Purpose

    Lentiviral vector plasmid expressing human zinc finger FYVE-type containing 27 (ZFYVE27) under the spleen focus-forming virus (SFFV) promoter and mCherry under the Ubiquitin C (UbC) promoter

    Depositor

    Bart Nieuwenhuis, Richard Eva

    Inserts

    ZFYVE27 (ZFYVE27 Human)
    mCherry
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    March 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET24c(+)-mtEFTu

    Plasmid

    #30535

    Depositor

    Linda Spremulli

    Article

    Woriax et al Biochim Biophys Acta. 1995 Dec 27. 1264(

    Insert

    Mature bovine mitochondrial elongation factor Tu
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Mature form amino acids 44-452. Mito import seque…

    Available Since

    Oct. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

14. pSG5 E107K T Ag

    Plasmid

    #133710

    Purpose

    Large T antigen mutant form in pSG5 vector for mammalian expression.

    Depositor

    James A. DeCaprio

    Article

    Zalvide et al Mol Cell Biol. 1995 Oct . 15(10):5800-10

    Insert

    SV40gp6 (SV40gp6 SV40)
    

    Expression

    Mammalian

    Mutation

    E107K

    Available Since

    April 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET3C-ARL16(CO)-His6

    Plasmid

    #216485

    Purpose

    Used for expression of human ARL16, short form, in bacteria. The open reading frame has been codon optimized for bacterial expression and contains a glycine rich linker and His6 tag at the C-terminus.

    Depositor

    Richard Kahn

    Insert

    ADP-ribosylation factor-like 16 (ARL16 Human)
    

    Tags

    includes GSGSGSHHHHHH (His6) tag at C-terminus

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJK370

    Plasmid

    #73595

    Purpose

    Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with C-terminal FLAG epitope and delta-12 fatty acid desaturase (FAD2-2) with C-terminal c-myc epitope

    Depositor

    T. J. Kappock

    Inserts

    delta-12 fatty acid acetylenase
    delta-12 fatty acid desaturase (form 2)
    

    Tags

    FLAG tag and c-myc tag

    Expression

    Yeast

    Mutation

    Silent mutation in Arg299 codon.

    Promoter

    GAL1 and GAL10

    Available Since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBABE puro K-Ras V12

    Plasmid

    #9052

    Purpose

    Retroviral expression of a constitutively active form of K-Ras

    Depositor

    William Hahn

    Insert

    KRAS (KRAS Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    G12V. Constitutively active.

    Available Since

    Nov. 14, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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18. pUC18/promEco/Lysostaphin

    Plasmid

    #125765

    Purpose

    Production of staphylolytic protein lysostaphin from a constitutive promoter

    Depositor

    Benedykt Wladyka

    Article

    Madry et al Mol Biotechnol. 2019 Oct;61(10):774-782.

    Insert

    lysostaphin
    

    Expression

    Bacterial

    Mutation

    sequence corresponding to the mature form protein

    Promoter

    pemIK-Sa1 promoter

    Available Since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET11a-SUMO3

    Plasmid

    #53143

    Purpose

    E.coli expression of untagged mature human SUMO3 (amino acids 1-93)

    Depositor

    Frauke Melchior

    Article

    Flotho et al Methods Mol Biol. 2012;832:93-110. doi:

    Insert

    SUMO3 (SUMO2 Human)
    

    Expression

    Bacterial

    Mutation

    mature form (amino acids 1-93)

    Promoter

    T7

    Available Since

    June 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET11a-SUMO2

    Plasmid

    #53142

    Purpose

    E.coli expression of untagged mature human SUMO2 (amino acids 1-92)

    Depositor

    Frauke Melchior

    Article

    Flotho et al Methods Mol Biol. 2012;832:93-110. doi:

    Insert

    SUMO2 (SUMO3 Human)
    

    Expression

    Bacterial

    Mutation

    mature form (amino acids 1-92)

    Promoter

    T7

    Available Since

    June 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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