We narrowed to 15,796 results for: form

1. pUC18/promSau/Lysostaphin

   Plasmid

   #125766

   Purpose

   Production of staphylolytic protein lysostaphin from a constitutive promoter

   Depositor

   Benedykt Wladyka

   Article

   Madry et al Mol Biotechnol. 2019 Oct;61(10):774-782.

   Insert

   lysostaphin
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   sequence corresponding to the mature form protein

   Promoter

   pemIK constitutive promoter

   Available since

   Oct. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA-RanWT-mRFP1-polyA

   Plasmid

   #59750

   Purpose

   Express wild-type form of Ran-mRFP1 in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

   Depositor

   Yi Zhang

   Article

   Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

   Insert

   Ran-mRFP1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and T7

   Available since

   Sept. 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLTR-SFFV-PTPN2 transcript variant 1-WPRE-UbC-Emerald

   Plasmid

   #201186

   Purpose

   Lentiviral vector plasmid expressing human PTPN2 transcript variant 1 under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

   Depositor

   Bart Nieuwenhuis

   Inserts

   PTPN2, transcript variant 1 (PTPN2 Human)
   Emerald
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDsEndoNA2

   Plasmid

   #174728

   Purpose

   Expresses DsRed-endoNA2 fusion protein for detection of polysialic acid. The endoNA2 is a catalytically inactive form of endosialidase from bacteriophage PK1A2.

   Depositor

   Jukka Finne

   Article

   Lehti et al Nat Commun. 2017 Dec 4;8(1):1915. doi: 1

   Insert

   DsRed-Monomer
   

   Use

   Tags

   Histag

   Expression

   Bacterial

   Mutation

   Promoter

   T5

   Available since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCLXEBR-pTF-kRasV12

   Plasmid

   #114318

   Purpose

   TET-inducible expression of the V12-mutant form of the ki-Ras protein

   Depositor

   Patrick Salmon

   Insert

   Ras (KRAS Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   G12V oncogenic mutation. Constitutively active K-…

   Promoter

   Available since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. CD44Z-c011

   Plasmid

   #175455

   Purpose

   Protein expression/secretion in mammalian cells. CD44s (standard form; exons 2-5, 15-16), A20-E649.

   Depositor

   Opher Gileadi

   Insert

   CD44 (CD44 Human)
   

   Use

   Tags

   His6

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1-Tras light chain

   Plasmid

   #216294

   Purpose

   Expresses anti-HER2 Tras Light chain in mammalian cells. To be paired with Tras heavy chain to form the anti-HER2 Tras Fab

   Depositor

   Mark Howarth

   Article

   Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

   Insert

   Tras light chain
   

   Use

   Affinity Reagent/ Antibody

   Tags

   Signal peptide

   Expression

   Mammalian

   Mutation

   Promoter

   T7 promoter

   Available since

   Aug. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-Pert Light chain

   Plasmid

   #216310

   Purpose

   Expresses anti-HER2 Pert Light chain in mammalian cells. To be paired with Pert heavy chain to form the anti-HER2 Pert Fab

   Depositor

   Mark Howarth

   Article

   Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

   Insert

   Pert Light chain
   

   Use

   Affinity Reagent/ Antibody

   Tags

   Signal peptide

   Expression

   Mammalian

   Mutation

   Promoter

   T7 promoter

   Available since

   Aug. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSL5200 (pDonor_ISGst3)

   Plasmid

   #210875

   Purpose

   Harbors a native ISGst3 transposon derived form Geobacillus stearothermophilus DSM 458

   Depositor

   Samuel H. Sternberg

   Article

   Meers et al Nature. 2023 Sep 27. doi: 10.1038/s41586

   Insert

   ISGst3
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. FRB-polySH35

    Plasmid

    #175234

    Purpose

    Bacterial expression and purification, mixed with polyPRM to form condensates, can bind FKBP in the presence of rapamycin

    Depositor

    Michael Rosen

    Article

    Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

    Insert

    NCK1 (NCK1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. GFP-Rab9aQ66L

    Plasmid

    #169072

    Purpose

    Express human constitutively active form of Rab9a(Q66L) in mammalian cells

    Depositor

    Gia Voeltz

    Article

    Wu et al Dev Cell. 2021 Jan 11;56(1):52-66.e7. do

    Insert

    Rab9a (RAB9A Human)
    

    Use

    Tags

    acGFP1

    Expression

    Mammalian

    Mutation

    Q66L

    Promoter

    CMV

    Available since

    Jan. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. FRB-polySH33

    Plasmid

    #175233

    Purpose

    Bacterial expression and purification, mixed with polyPRM to form condensates, can bind FKBP in the presence of rapamycin

    Depositor

    Michael Rosen

    Article

    Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

    Insert

    NCK1 (NCK1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. RISAP act beta-catenin (CT#1147)

    Plasmid

    #14041

    Depositor

    Cliff Tabin

    Article

    Kardon et al Dev Cell. 2003 Dec . 5(6):937-44.

    Insert

    beta catenin (ctnnb1.L Frog)
    

    Use

    Retroviral; Avian expression

    Tags

    Expression

    Mutation

    Activated form

    Promoter

    Available since

    Feb. 16, 2007

    Availability

    Academic Institutions and Nonprofits only

14. dnCEBP: pLenti-TRE-A-C/EBP-PGK-rtTAA-2A-Puro

    Plasmid

    #177201

    Purpose

    encoding a dominant negative form of CEBP under inducible promoter

    Depositor

    Markus Covert

    Article

    Kudo et al bioRxiv (2022)

    Insert

    dnCEBP (A-C/EBP)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSG5-mERRbeta-short

    Plasmid

    #52188

    Purpose

    Expresses mouse ERRbeta short form splice variant in mammalian cells

    Depositor

    Rebecca Riggins

    Article

    Heckler et al Cell Cycle. 2014 Dec 12:0.

    Insert

    Estrogen-related receptor beta (Esrrb Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    April 24, 2014

    Availability

    Academic Institutions and Nonprofits only

16. V5-W279A-short-UBASH3A

    Plasmid

    #192086

    Purpose

    Mammalian expression of human W279A short-form UBASH3A with V5 tag

    Depositor

    Patrick Concannon

    Article

    Ge et al J Immunol. 2019 Dec 1;203(11):2827-2836.

    Insert

    UBASH3A (UBASH3A Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    W279A

    Promoter

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJK371

    Plasmid

    #73596

    Purpose

    Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with C-terminal FLAG epitope and delta-12 fatty acid desaturase (FAD2-3) with C-terminal c-myc epitope

    Depositor

    T. J. Kappock

    Inserts

    delta-12 fatty acid acetylenase
    delta-12 fatty acid desaturase (form 3)
    

    Use

    Tags

    FLAG tag and c-myc tag

    Expression

    Yeast

    Mutation

    Point mutants in FAD2-3 ORF relative to deposited…

    Promoter

    GAL1 and GAL10

    Available since

    March 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJK273

    Plasmid

    #71563

    Purpose

    Produces Crepis alpina delta12 fatty acid desaturase (FAD2-2) in yeast

    Depositor

    T. J. Kappock

    Article

    Nam et al J Exp Bot. 2007;58(6):1421-32. Epub 2007

    Insert

    delta12 fatty acid desaturase (form 2)
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    GAL1

    Available since

    Jan. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. delta NT delta CT p70S6K1 MSCV puro

    Plasmid

    #8463

    Depositor

    David Sabatini

    Article

    Ali et al J Biol Chem 2005 Apr 4;.

    Insert

    delta NT delta CT p70 S6K1 (Rps6kb1 Rat)
    

    Use

    Retroviral

    Tags

    myc

    Expression

    Mammalian

    Mutation

    p70 s6k1 form

    Promoter

    Available since

    June 20, 2005

    Availability

    Academic Institutions and Nonprofits only

20. pLV-SFFV-ZFYVE27-WPRE-UbC-Emerald

    Plasmid

    #214469

    Purpose

    Lentiviral vector plasmid expressing human zinc finger FYVE-type containing 27 (ZFYVE27) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

    Depositor

    Richard Eva, Bart Nieuwenhuis

    Inserts

    ZFYVE27 (ZFYVE27 Human)
    Emerald
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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