We narrowed to 786 results for: PFL

1. FLAG-Cdk9 T186A

   Plasmid

   #28101

   Depositor

   Andrew Rice

   Article

   Wang et al J Biol Chem. 2008 Nov 28. 283(48):33578-

   Insert

   Cdk9 (CDK9 Human)
   

   Use

   Tags

   FLAG

   Expression

   Bacterial and Mammalian

   Mutation

   Contains a T186A mutation in Cdk9

   Promoter

   Available since

   March 15, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMA_Int

   Plasmid

   #110096

   Purpose

   E. coli replicative vector which contains the mycophage integrase gene required for the attP/attB integration. Is co-transformed in trans together with pFLAG_attP, but cannot replicate in mycobacteria, and is thus lost shortly after electroporation.

   Depositor

   Markus Seeger

   Article

   Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

   Insert

   mycophage integrase
   

   Use

   Required for transformation of attb integrating v…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSF1C-A-RFP

   Plasmid

   #172431

   Purpose

   StrepFlex MoClo vector for cloning in E. coli and Streptomyces expression - equivalent of pTU1-A-RFP from EcoFlex (ACS Synth. Biol. 2016, 5, 10, 1059–1069)

   Depositor

   Paul Freemont, Simon Moore

   Article

   Moore et al ACS Synth Biol. 2021 Feb 19;10(2):402-41

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   None

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSF2C-A-RFP

   Plasmid

   #172432

   Purpose

   StrepFlex MoClo vector for E.coli cloning and Streptomyces chromosomal integration - equivalent of pTU2-A-RFP from EcoFlex (ACS Synth. Biol. 2016, 5, 10, 1059–1069)

   Depositor

   Paul Freemont, Simon Moore

   Article

   Moore et al ACS Synth Biol. 2021 Feb 19;10(2):402-41

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   None

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBbdCas9S(-10TC)_Psyn-sgRNA500

   Plasmid

   #149583

   Purpose

   parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

   Depositor

   Christine Jacobs-Wagner

   Article

   Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PflaB, PpQE30, Psyn

   Available since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBbdCas9S(-10AC12)_Psyn-sgRNArodA

   Plasmid

   #149661

   Purpose

   all-in-one CRISPRi vector for targeting B. burgdorferi rodA

   Depositor

   Christine Jacobs-Wagner

   Article

   Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

   Insert

   dCas9, lacI, sgRNArodA
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PflaB, PpQE30(-10AC12), Psyn

   Available since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBbdCas9S(RBSmut)_Psyn-sgRNAflaB

   Plasmid

   #149588

   Purpose

   all-in-one CRISPRi vector for targeting B. burgdorferi flaB

   Depositor

   Christine Jacobs-Wagner

   Article

   Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

   Insert

   dCas9, lacI, sgRNAflaB
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PflaB, PpQE30, Psyn

   Available since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBbdCas9S(RBSmut)_Psyn-sgRNAftsI

   Plasmid

   #149590

   Purpose

   all-in-one CRISPRi vector for targeting B. burgdorferi ftsI

   Depositor

   Christine Jacobs-Wagner

   Article

   Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

   Insert

   dCas9, lacI, sgRNAftsI
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PflaB, PpQE30, Psyn

   Available since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBbdCas9S(RBSmut)_Psyn-sgRNAmreB

   Plasmid

   #149594

   Purpose

   all-in-one CRISPRi vector for targeting B. burgdorferi mreB

   Depositor

   Christine Jacobs-Wagner

   Article

   Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

   Insert

   dCas9, lacI, sgRNAmreB
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PflaB, PpQE30, Psyn

   Available since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-M2-TLR2-V660N

    Plasmid

    #12292

    Depositor

    Bruce Beutler

    Article

    Jiang et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

    Insert

    TLR2 (Tlr2 Mouse)
    

    Use

    Tags

    FLAG (FLAG-M2)

    Expression

    Mammalian

    Mutation

    M82I, H202Y and V660N; signal peptide (aa1-23) re…

    Promoter

    Available since

    Jan. 25, 2007

    Availability

    Academic Institutions and Nonprofits only