We narrowed to 4,386 results for: ARA-2

1. pEN-R2-mCsfGFP-L3

   Plasmid

   #178602

   Purpose

   It can be used to study protein turnover in living plant cells of Arabidopsis (Arabidopsis thaliana) and Nicotiana benthamiana.

   Depositor

   Frederica Theodoulou

   Article

   Zhang et al Plant Physiol. 2019 Jun;180(2):718-731.

   Insert

   Tandem fluorescent protein timers (tFTs)
   

   Use

   Entry vector for gateway cloning

   Tags

   mCherry superfolder GFP

   Available Since

   Dec. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMTTH+eGFP-bromo5(N140A)

   Plasmid

   #157806

   Purpose

   Expression of synthetic protein composed of eGFP and 5 repeats of BRD4 bromodomain #1 with N140A mutation

   Depositor

   Michael Rosen

   Article

   Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

   Insert

   eGFP-bromo5(N140A)
   

   Tags

   His6 and MBP

   Expression

   Bacterial

   Mutation

   N140A

   Promoter

   pTAC

   Available Since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET30a-SRAP

   Plasmid

   #84524

   Purpose

   Expresses murine SRAP domain (250 aa) in bacteria

   Depositor

   Douglas Feldman

   Article

   Kweon et al Cell Rep. 2017 Oct 10;21(2):482-494. doi

   Insert

   SRAP domain (Hmces Mouse)
   

   Tags

   6xHis and S-Tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFX-GalT-SECFP

   Plasmid

   #174470

   Purpose

   Express SECFP with golgi apparatus-targeting sequence in mammalian cells

   Depositor

   Yusuke Ohba

   Article

   Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

   Insert

   SECFP with golgi apparatus-targeting sequence
   

   Tags

   β1,4-galactose transferase

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFX-GalT-iRFP

   Plasmid

   #174474

   Purpose

   Express iRFP with golgi apparatus-targeting sequence in mammalian cells

   Depositor

   Yusuke Ohba

   Article

   Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

   Insert

   iRFP with golgi apparatus-targeting sequence
   

   Tags

   β1,4-galactose transferase

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFX-GalT-Venus

   Plasmid

   #174472

   Purpose

   Express Venus with golgi apparatus-targeting sequence in mammalian cells

   Depositor

   Yusuke Ohba

   Article

   Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

   Insert

   Venus with golgi apparatus-targeting sequence
   

   Tags

   β1,4-galactose transferase

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFSynWSyt1C2AB(80 JuxtaK-421)msGFP

   Plasmid

   #209016

   Purpose

   Overexpression of truncated syt1 with Juxta K mutations and tagged with GFP in mammalian cells

   Depositor

   Edwin Chapman

   Article

   Mehta et al Nat Commun. 2024 Jan 4;15(1):262. doi: 1

   Insert

   syt1 (Syt1 Rat)
   

   Use

   Lentiviral

   Tags

   msfGFP

   Expression

   Mammalian

   Mutation

   aa 80-421 only, Juxta K mutant (80KKCLFKKKNKKKGKE…

   Available Since

   Nov. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pETSyt1C2AB(80 JuxtaK-421)msGFP

   Plasmid

   #209011

   Purpose

   Recombinant protein expression of truncated syt1 construct with Juxta K mutations and tagged with GFP

   Depositor

   Edwin Chapman

   Article

   Mehta et al Nat Commun. 2024 Jan 4;15(1):262. doi: 1

   Insert

   syt1 (Syt1 Rat)
   

   Tags

   6xHis and msfGFP

   Expression

   Bacterial

   Mutation

   aa 80-421 only, Juxta K mutant (80KKCLFKKKNKKKGKE…

   Available Since

   Nov. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pmCherry-TdIF2

   Plasmid

   #196906

   Purpose

   visualization of TdIF2/ERBP as a fusion to mCherry

   Depositor

   Kenji Sugimoto

   Article

   Fukada et al Biosci Biotechnol Biochem. 2019 Jul;83(7

   Insert

   TdIF2
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEGFP-TdIF2

    Plasmid

    #199699

    Purpose

    visualization of nucleolus in living mammalian cells

    Depositor

    Kenji Sugimoto

    Article

    Fukada et al Biosci Biotechnol Biochem. 2019 Jul;83(7

    Insert

    TdIF2
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAc5.1B-EGFP-DmHPat_57-968-dsRNAres_J

    Plasmid

    #146690

    Purpose

    Insect Expression of DmHPat_57-968-dsRNAres

    Depositor

    Elisa Izaurralde

    Article

    Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

    Insert

    DmHPat_57-968-dsRNAres (Patr-1 Fly)
    

    Expression

    Insect

    Mutation

    one silent mutation A1758G and one non silent G56…

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAc5.1B-lambdaN-HA-DmHPat_57-968-del500-684_J

    Plasmid

    #146695

    Purpose

    Insect Expression of DmHPat_57-968-del500-684

    Depositor

    Elisa Izaurralde

    Article

    Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

    Insert

    DmHPat_57-968-del500-684 (Patr-1 Fly)
    

    Expression

    Insect

    Mutation

    two silent mutations compared to the sequence giv…

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAc5.1B-HA-DmHPat_57-968-dsRNAres_H

    Plasmid

    #146464

    Purpose

    Insect Expression of DmHPat_57-968-dsRNAres

    Depositor

    Elisa Izaurralde

    Article

    Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

    Insert

    DmHPat_57-968-dsRNAres (Patr-1 Fly)
    

    Expression

    Insect

    Mutation

    one silent mutation T477C and one non silent muta…

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. GFP-CBS (H65R)

    Plasmid

    #182924

    Purpose

    Mammalian expression plasmid for human CBS mutant H65R

    Depositor

    Philip Eaton

    Article

    Switzer et al Proc Natl Acad Sci U S A. 2023 Jan 17;12

    Insert

    Cystathionine Beta-Synthase (CBS Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    changed histidine 65 to arginine

    Promoter

    CMV

    Available Since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDEST-pSP172BSSPE-Swa1::HA-RfA

    Plasmid

    #186404

    Purpose

    Destination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of N-ter HA tag and a gene of interest under control of a reg. sequence in the Swa1 restriction site.

    Depositor

    Patrick Lemaire

    Article

    Roure et al PLoS One. 2007. 2(9):e916.

    Type

    Empty backbone

    Use

    Gateway destination vector

    Available Since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSCL.108

    Plasmid

    #184977

    Purpose

    Express -Eco1 LYP1 editing ncRNA and gRNA

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1 editing ncRNA and gRNA, LYP1 E27X, a1/a2 length: 12
    

    Expression

    Yeast

    Mutation

    LYP1 donor E27stop

    Promoter

    Gal7

    Available Since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSCL.106

    Plasmid

    #184975

    Purpose

    Express -Eco1 CAN1 editing ncRNA and gRNA

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1 editing ncRNA and gRNA, CAN1 G444X, a1/a2 length: 12
    

    Expression

    Yeast

    Mutation

    CAN1 donor G444stop

    Promoter

    Gal7

    Available Since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSCL.037

    Plasmid

    #184966

    Purpose

    Express -Eco1 dead RT and ncRNA in yeasst

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1: RT and ncRNA(wt), a1/a2 length: 12, dead RT
    

    Expression

    Yeast

    Mutation

    Human codon optimized RT, YXDD catalytic region m…

    Promoter

    Gal7

    Available Since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSCL.112

    Plasmid

    #184981

    Purpose

    Express -Eco1 FAA1 editing ncRNA and gRNA

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1 editing ncRNA and gRNA, FAA1 P233X, a1/a2 length: 12
    

    Expression

    Yeast

    Mutation

    FAA1 donor P233stop

    Promoter

    Gal7

    Available Since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSCL.002

    Plasmid

    #184972

    Purpose

    Express -Eco1 ADE2 editing ncRNA and gRNA

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1 editing ncRNA and gRNA, ADE2 P272X, a1/a2 length: 12
    

    Expression

    Yeast

    Mutation

    ADE2 donor P272stop

    Promoter

    Gal7

    Available Since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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