Addgene

We narrowed to 21,471 results for: ESC;

Showing: 1461 - 1480 of 21471 results

Results per page:

Sort results by:

Sirius/pRSETB

Plasmid

#51956

Purpose

bacterial expression of Sirius, a pH-insensitive and photostable ultramarine fluorescent protein

Depositor

Takeharu Nagai

Article

Tomosugi et al Nat Methods. 2009 May;6(5):351-3. doi: 1

Insert

Sirius

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

April 15, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSetB-Peredox

Plasmid

#73806

Purpose

Expresses the Peredox fluorescent sensor protein (for NADH/NAD ratio) in bacteria, with N-terminal his-tag

Depositor

Gary Yellen

Article

Mongeon et al Antioxid Redox Signal. 2016 Mar 18.

Insert

Peredox

Use

Tags

his-tag to facilitate purification

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 30, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pBA2219

Plasmid

#38152

Depositor

David Waugh

Article

Austin et al Protein Expr Purif. 2012 Mar;82(1):116-2

Insert

DsbC (dsbC E. coli)

Use

Tags

N-terminal leader peptide (cloning artifact)

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 21, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pACYC-Skp-TF

Plasmid

#83921

Purpose

Co-expresses cytoplasmic copies of the Skp and Trigger Factor protein folding chaperones.

Depositor

Karl Griswold

Article

Lamppa et al J Biotechnol. 2013 Mar 10;164(1):1-8. do

Inserts

Skp
Trigger Factor

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Ec2Avs2

Plasmid

#188790

Purpose

Avs2 from Escherichia coli NCTC10650

Depositor

Feng Zhang

Article

Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

Insert

Ec2Avs2

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Skylan-S-N1

Plasmid

#129737

Purpose

A green photo-switchable fluorescent protein for super-resolution optical fluctuation imaging (SOFI)

Depositor

Pingyong Xu

Article

Zhang et al ACS Nano. 2015 Mar 24;9(3):2659-67. doi:

Type

Empty backbone

Use

Tags

Skylan-S

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pmiRFP709-N1

Plasmid

#79989

Purpose

Monomeric near-infrared fluorescent protein miRFP709 in mammalian plasmid

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

Insert

miRFP709

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA 3.1(-) VSFP3.1

Plasmid

#18951

Depositor

Thomas Knopfel

Article

Lundby et al PLoS ONE. 2008 . 3(6):e2514.

Insert

VSFP3.1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 30, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSCVpuro-mIFP

Plasmid

#96951

Purpose

Retroviral plasmid containing mIFP for labeling of cells

Depositor

Lee Grimes

Article

Kleeman et al Cytometry A. 2018 Mar 13. doi: 10.1002/c

Insert

Monomeric Infrared Fluorescent Protein

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCIS.MTRIA-OT

Plasmid

#184596

Purpose

For expression of a fluorescent sensor for oxytocin in mammalian cells

Depositor

Daisuke Ino, Hiroshi Hibino

Article

Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

Insert

MTRIA-OT

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG promoter

Available since

June 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
vgCam-pRSETb

Plasmid

#193362

Purpose

A FRET-based calcium probe using Sumire

Depositor

Takeharu Nagai

Article

Sugiura et al Commun Biol. 2022 Nov 3;5(1):1172. doi:

Insert

vgCam

Use

E.coli expression

Tags

His X6 tag and Strep tag

Expression

Mutation

Promoter

T7

Available since

Feb. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
csr-1_hH1_mEos3.1_V5

Plasmid

#191750

Purpose

Photoconvertible fluorescent nuclear reporter.

Depositor

Jay C Dunlap

Article

Wang et al Fungal Genet Biol. 2022 Dec 5:103763. do

Insert

hH1 (hH1 Neurospora crassa)

Use

Tags

V5, ccg-1 promoter, and mEos3.1

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCas1+2

Plasmid

#72676

Purpose

Express Cas1 and Cas2 for the CRISPR adaptation process in Escherichia coli. Has streptomycin resistance.

Depositor

Udi Qimron

Article

Yosef et al Nucleic Acids Res. 2012 Jul;40(12):5569-

Insert

Cas1, Cas2

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 20, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pET22-HT/spoR

Plasmid

#73631

Purpose

Produces Escherichia coli guanylate kinase (SpoR), N-terminal His6 tag

Depositor

T. J. Kappock, James Williamson

Article

Schultheisz et al ACS Chem Biol. 2008 Aug 15;3(8):499-511.

Insert

guanylate kinase

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS-TRE-mTurquoise2-WPRE

Plasmid

#104103

Purpose

Expresses mTurquoise2 under the TRE promoter in mammalian cells.

Depositor

Takeshi Imai

Article

Sakaguchi et al eLife. 2018 Nov 20;7. pii: 40350. doi: 1

Insert

mTurquoise2

Use

Tags

Expression

Mammalian

Mutation

Promoter

TRE

Available since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
EcAvs4

Plasmid

#188794

Purpose

Avs4 from Escherichia coli NCTC11132

Depositor

Feng Zhang

Article

Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

Insert

EcAvs4

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-BRIPO

Plasmid

#215542

Purpose

BRIPO (Bioluminescent Red Indicator for Potassium)

Depositor

Huiwang Ai

Article

Zhao et al J Am Chem Soc. 2024 May 15;146(19):13406

Insert

BRIPO

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC19-T7-SdB-T

Plasmid

#87307

Purpose

Expresses the fluorescence-activating Stabilized dimeric Broccoli (SdB) aptamer under the control of the T7 promoter.

Depositor

Donald Burke

Article

Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102

Insert

Stabilized dimeric Broccoli aptamer

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSCVpuro-Sapphire

Plasmid

#96950

Purpose

Retroviral plasmid containing Sapphire for labeling of cells

Depositor

Lee Grimes

Article

Kleeman et al Cytometry A. 2018 Mar 13. doi: 10.1002/c

Insert

Sapphire fluorescent protein

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pMAM-RecG-ST2TaV-EGFP

Plasmid

#31275

Depositor

Andrew Pierce

Article

Killen et al BMC Mol Biol. 2012 Oct 30;13:33. doi: 10

Insert

RecG-ST2-TaV-EGFP

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 18, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge