Addgene

We narrowed to 8,185 results for: FIE

Showing: 1461 - 1480 of 8185 results

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pGL3-Gateway-DSCP

Plasmid

#71506

Purpose

flySTARR-seq validation vector with Gateway cassette upstream of the firefly luciferase gene and the DSCP core promoter

Depositor

Alexander Stark

Article

Arnold et al Science. 2013 Mar 1;339(6123):1074-7. do

Type

Empty backbone

Use

Flystarr-seq validation vector

Tags

luciferase

Expression

Insect

Mutation

Promoter

Available since

Dec. 29, 2015

Availability

Academic Institutions and Nonprofits only

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PB-CMV-NLS-dCas9-NLS

Plasmid

#99908

Purpose

For the expression of NLS-dCas9-NLS

Depositor

Yujie Sun

Article

Shao et al Nucleic Acids Res. 2016 May 19;44(9):e86

Insert

NLS-dCas9-NLS

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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SLC30A10-GFP

Plasmid

#104381

Purpose

Expresses SLC30A10-GFP wild-type in mammalian cells

Depositor

Somshuvra Mukhopadhyay

Article

Selyunin et al J Cell Biol. 2017 Oct 2;216(10):3249-326

Insert

SLC30A10 (SLC30A10 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB2-SA(1)-T2A-Gal4

Plasmid

#62900

Purpose

Creating Gal4 drivers from MiMIC lines with inserts into coding introns in Phase 1

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-Gal4

Use

Tags

Expression

Mutation

Promoter

Available since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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pCEP4-SERT-myc

Plasmid

#107456

Purpose

Mammalian expression plasmid for codon optimized hSERT with internal MYC tag inserted in ECL2

Depositor

Erik Procko

Article

Young et al bioRxiv 2021.04.19.440442

Insert

Serotonin Transporter (SERT) (SLC6A4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 14, 2018

Availability

Academic Institutions and Nonprofits only

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CP1012-ABEmax

Plasmid

#119806

Purpose

A-to-G base editor

Depositor

David Liu

Article

Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

Insert

ecTadA-ecTadA*(7.10)-nSpCas9 (CP1012)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB2-SA(2)-T2A-Gal4

Plasmid

#62901

Purpose

Creating Gal4 drivers from MiMIC lines with inserts into coding introns in Phase 2

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-Gal4

Use

Tags

Expression

Mutation

Promoter

Available since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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pAJT248

Plasmid

#202289

Purpose

RPU integration plasmid (LPattB2)

Depositor

Christopher Voigt

Article

Triassi et al Cell Syst. 2023 Jun 21;14(6):512-524.e12

Insert

J23101-RiboJ00-B0034-YFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pET22b-GFP-CPDSalI

Plasmid

#38257

Depositor

Aimee Shen, Matthew Bogyo

Article

Shen et al PLoS One. 2009 Dec 2;4(12):e8119.

Insert

eGFP

Use

Tags

6xHis and Vibrio cholerae MARTX toxin cysteine pr…

Expression

Bacterial

Mutation

cloned from pEGFPN3 (Clonetech)

Promoter

T7

Available since

July 20, 2012

Availability

Academic Institutions and Nonprofits only
Lentiviral-FKBP-cpGFP-Nb39

Plasmid

#191498

Purpose

Rapamycin-dependent opioid sensor for opioid receptors (component 2)

Depositor

Wenjing Wang

Article

Kroning et al ACS Chem Biol. 2022 Aug 19;17(8):2212-22

Insert

FKBP-cpGFP-Nb39

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CMV

Available since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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plYJP067-Ptet

Plasmid

#202299

Purpose

PTet-YFP plasmid for LPattB2 integration

Depositor

Christopher Voigt

Article

Triassi et al Cell Syst. 2023 Jun 21;14(6):512-524.e12

Insert

Ptet-YFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST40-MFRN2

Plasmid

#50546

Purpose

Expression of MFRN2 gene in mammalian cells

Depositor

Vamsi Mootha

Article

Baughman et al Nature. 2011 Jun 19. ():.

Insert

Mrfn2 (SLC25A28 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 13, 2014

Availability

Academic Institutions and Nonprofits only
pDEST14-SpyCatcher EQ

Plasmid

#35045

Depositor

Mark Howarth

Article

Zakeri et al Proc Natl Acad Sci U S A. 2012 Mar 20;10

Insert

SpyCatcher EQ

Use

Tags

His6

Expression

Bacterial

Mutation

Deletion of C-terminal till I113, and point mutat…

Promoter

Available since

April 18, 2012

Availability

Academic Institutions and Nonprofits only
CP1249-CBEmax

Plasmid

#119804

Purpose

C-to-T base editor

Depositor

David Liu

Article

Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

Insert

rApobec1-nSpCas9 (CP1249) -UGI-UGI

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

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pMH-EF1-GFP

Plasmid

#133018

Purpose

(Empty Backbone) Gateway-compatible destination vector for mammalian expression of N-terminal GFP protein under EF1 promoter.

Depositor

Michael Huen

Article

Dong et al Proc Natl Acad Sci U S A. 2020 Jul 21;11

Type

Empty backbone

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

EF1

Available since

Dec. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Mito-AcGFP-MTn

Plasmid

#154350

Purpose

Mammalian expression of MTn

Depositor

Wanzhong He

Article

Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

Insert

MTn

Use

Tags

AcGFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pCFJ1201

Plasmid

#44483

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Insert

Minimal Mos1, unc-119, peel-1, MCS

Use

Multiple cloning site vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2013

Availability

Academic Institutions and Nonprofits only

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pACT-Lex

Plasmid

#183149

Purpose

Yeast-two-hybrid AD vector with 4XLexOp (activated by Lex-ER-B112)

Depositor

Assaf Mosquna

Article

Feuer et al ACS Synth Biol. 2022 Jul 27. doi: 10.102

Insert

GAL4 AD

Use

Yeat two-hybrid

Tags

HA tag

Expression

Yeast

Mutation

Promoter

Available since

July 29, 2022

Availability

Academic Institutions and Nonprofits only

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SPI1_pet28a

Plasmid

#131653

Purpose

Bacterial expression of ETS gene SPI1

Depositor

Peter Hollenhorst

Article

Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

Insert

SPI1 (SPI1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 4, 2019

Availability

Academic Institutions and Nonprofits only

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GLUT9-eGFP/pcDNA-DEST47

Plasmid

#18730

Depositor

Wolf Frommer

Article

Takanaga et al Biochim Biophys Acta. 2008 Apr . 1778(4)

Insert

GLUT9-eGFP/pcDNA-DEST47 (SLC2A9 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

deleted TAA

Promoter

Available since

July 3, 2008

Availability

Academic Institutions and Nonprofits only