Addgene

Showing: 1461 - 1480 of 3078 results

Results per page:

Sort results by:

pCAGGS-mouse PKA-Calpha-mEGFP

Plasmid

#45528

Depositor

Haining Zhong

Article

Zhong et al Neuron. 2009 May 14;62(3):363-74. doi: 1

Insert

PKA catalytic subunit alpha tagged by mEGFP

Use

With chicken beta actin promoter and wpre

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 12, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
CMV Marina-T2A-nls-mCherry

Plasmid

#74216

Purpose

genetically-encoded fluorescent voltage indicator

Depositor

Vincent Pieribone

Article

Platisa et al ACS Chem Neurosci. 2017 Jan 3. doi: 10.1

Insert

fluorescent protein voltage sensor

Use

Tags

mCherry

Expression

Mammalian

Mutation

Ci-VSP contains R217Q mutation; super ecliptic pH…

Promoter

CMV

Available since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
hSyn Marina-T2A-nls-mCherry

Plasmid

#85843

Purpose

genetically-encoded fluorescent voltage indicator

Depositor

Vincent Pieribone

Article

Platisa et al ACS Chem Neurosci. 2017 Jan 3. doi: 10.1

Insert

GEVI Marina

Use

Tags

mCherry

Expression

Mammalian

Mutation

Ci-VSP contains R217Q mutation; super ecliptic pH…

Promoter

hSyn1

Available since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-Casp7 C186A-Flag

Plasmid

#11816

Depositor

Guy Salvesen

Article

Stennicke et al J Biol Chem. 1997 Oct 10. 272(41):25719-

Insert

Caspase 7 catalytic mutant (CASP7 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

C186A

Promoter

Available since

May 12, 2006

Availability

Academic Institutions and Nonprofits only
pDUP3

Plasmid

#92174

Purpose

Vector containing the two fragments comprising the 550 bp NEUT5L region with NAT1 marker. Integration by duplication.

Depositor

Judith Berman

Article

Gerami-Nejad et al Microbiology. 2013 Mar;159(Pt 3):565-79.

Insert

NAT1

Use

Genbank: u03440.1

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 11, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
LNCX TbetaRII DN

Plasmid

#12640

Depositor

Rik Derynck

Article

Choy et al J Cell Biol. 2000 May 1. 149(3):667-82.

Insert

TGF-beta receptor II (TGFBR2 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Dominant negative: truncated cytoplasmically

Promoter

Available since

Oct. 10, 2006

Availability

Academic Institutions and Nonprofits only
pRSF_6His-ZmSUMO1a-GG

Plasmid

#91929

Purpose

Bacterial expression of maize SUMO1a

Depositor

Richard Vierstra

Article

Augustine et al Plant Physiol. 2016 Jul;171(3):2191-210.

Insert

6His-ZmSUMO1a-GG

Use

Tags

6His

Expression

Bacterial

Mutation

Truncation of ZmSUMO1a after the diGlycine motif

Promoter

Available since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-6xHis-GFP(-6)-H2_mCherry(-6)-L1

Plasmid

#205050

Purpose

Co-expressed fluorescent proteins with charge-patterned cationic peptide to promote multi-protein biomolecular condensate formation in E. coli

Depositor

Allie Obermeyer

Article

Liao et al ACS Synth Biol. 2024 Feb 3. doi: 10.1021

Insert

GFP(-6)-H2_mCherry(-6)-L1

Use

Tags

GRRGKKSRK and MGHHHHHGGA

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-6xHis-GFP(-6)-H2_mCherry(-6)-L2

Plasmid

#205051

Purpose

Co-expressed fluorescent proteins with charge-patterned cationic peptide to promote multi-protein biomolecular condensate formation in E. coli

Depositor

Allie Obermeyer

Article

Liao et al ACS Synth Biol. 2024 Feb 3. doi: 10.1021

Insert

GFP(-6)-H2_mCherry(-6)-L2

Use

Tags

GRRGKKSRKGRRGKKSRK and MGHHHHHGGA

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMCP-Luc (MCP-1 promoter in pGL3-basic)

Plasmid

#40324

Depositor

Alexander Dent

Article

Toney et al Nat Immunol. 2000 Sep;1(3):214-20.

Insert

MCP-1 promoter (Ccl2 Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

contains MCP-1 promoter region through the nucleo…

Promoter

MCP-1

Available since

Sept. 28, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28b-HRASLS3-1-132

Plasmid

#100725

Purpose

Expression of N-terminal region of human HRASLS3 in E.coli

Depositor

Byung Il Lee

Article

Han et al Protein Expr Purif. 2010 May;71(1):103-7

Insert

HRASLS3 (PLAAT3 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7 promotor

Available since

Oct. 31, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
DCT.VV.Orf35

Plasmid

#12723

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf35 (J3R Vaccinia Virus)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 1, 2006

Availability

Academic Institutions and Nonprofits only
pMSCV-FKBP-TRIF(trunc 1-63)

Plasmid

#221249

Purpose

Expression of TRIF truncation 3 (64-222) in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

TRIF truncation 3 (64-222) (Ticam1 Mouse)

Use

Retroviral

Tags

3xFLAG-4xFKBP

Expression

Mutation

Promoter

Available since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pRDB_131

Plasmid

#216095

Purpose

Cas12a [EnAs] CRISPRa targeting CD97, CD4, CD26, CD274, positive control

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

CD97, CD4, CD26, CD274 guides

Use

CRISPR and Lentiviral; Positive controls

Tags

Expression

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
DCT.VV.Orf200

Plasmid

#12888

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf200

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 29, 2006

Availability

Academic Institutions and Nonprofits only
pAM/CBA-eGFP-miRNegativex3-WPRE-bGHpA

Plasmid

#194249

Purpose

Expresses 3 non-targeting miRNAs

Depositor

Michael J Fox Foundation MJFF

Insert

miRNegative

Use

AAV and RNAi

Tags

Expression

Mutation

Promoter

CAG

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-hSyn-DIO-jGCaMP8s-P2A-ChrimsonR-ST

Plasmid

#174007

Purpose

Cre dependent co-expression of jGCaMP8s and soma-targeted ChrimsonR under the control of an hSyn promoter. jGCaMP8s and ChrimsonR are separated by cleavable peptide sequence P2A.

Depositor

Mark Histed

Article

LaFosse et al eNeuro. 2023 Mar 29;10(3):ENEURO.0378-22

Has Service

AAV9

Insert

jGCaMP8s-P2A-ChrimsonR-ST

Use

AAV

Tags

6xHis and soma targeting motif

Expression

Mutation

Promoter

hSyn

Available since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pGLO-GFP-1UAG

Plasmid

#82500

Purpose

Expresses GFP with 1 UAG codon at the amino acid position 3

Depositor

Chang Liu

Article

Schwessinger et al Integr Biol (Camb). 2016 Apr 18;8(4):542

Insert

Green Fluorescent Protein with 1 UAG codon

Use

Tags

Expression

Bacterial

Mutation

Added an UAG codon at the amino acid position 3

Promoter

AraBAD

Available since

Sept. 22, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-6XHis-SUMO-SIRT2

Plasmid

#102622

Purpose

Expresses a truncation (aa38-356) of human SIRT2 isoform 1 in bacteria for purification

Depositor

Hening Lin

Article

Jing et al Cancer Cell. 2016 Mar 14;29(3):297-310.

Insert

SIRT2 (SIRT2 Human)

Use

Tags

6XHis-SUMO

Expression

Bacterial

Mutation

Deleted amino acids 1-37 and 357-389

Promoter

Available since

Feb. 1, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
ZNF217_pLX307

Plasmid

#98384

Purpose

Lentiviral expression of ZNF217

Depositor

William Hahn, Sefi Rosenbluh

Article

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

ZNF217 (ZNF217 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

E1Fa

Available since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge