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We narrowed to 563 results for: cat.3

Showing: 1 - 20 of 563 results

  1. Immunology Research Plasmids and Resources

    Type
    Collection
    ...immunoglobulin heavy diversity 3-22 IGHD322 IGHD3-3 immunoglobulin heavy diversity 3-3 DXP4, IGHD33 IGHD3-9 immunoglobulin...VH IGHV3-30-3 immunoglobulin heavy variable 3-30-3 IGHV3-3, IGHV3303 IGHV3-30-5 immunoglobulin heavy variable...motif) ligand 3-like 2 G0S19-3, LD78gamma, SCYA3L2 CCL3L3 chemokine (C-C motif) ligand 3-like 3 464.2, D17S1718... variable 3-30-5 IGHV3-3, IGHV3305 IGHV3-33 immunoglobulin heavy variable 3-33 IGHV333, VH IGHV3-35 immunoglobulin...lambda variable 3-9 (gene/pseudogene) IGLV39, V2-6 IGLV4-3 immunoglobulin lambda variable 4-3 IGLV43, V5-1..., MRS DEFA3 defensin, alpha 3, neutrophil-specific DEF3, HNP-3, HNP3, HP-3 DEFA5 defensin, alpha 5, Paneth...

  2. p53 Pathway

    Type
    Collection
    ...each gene page are provided below. Symbol Name 14-3-3-σ Stratifin Apaf-1 Apoptotic peptidase activating...CCNB2 CCNB3 Cyclin B1, 2, or 3 Cyclin D CCND1 CCND2 CCND3 Cyclin D1, 2, or 3 Cyclin E CCNE1 CCNE2 Cyclin...BH3 interacting domain death agonist CASP3 Caspase 3, apoptosis-related cysteine peptidase CASP8 Caspase...IGF-BP3 Insulin-like growth factor binding protein 3 KAI CD82 molecule Maspin Serpin family B member 5 ...reductase M2 B (RRM2B) PAG608 Zinc finger, matrin-type 3 PAI Serpin peptidase inhibitor, clade E (nexin, plasminogen...Phosphatase and tensin homolog PUMA BCL2 binding component 3 Reprimo TP53 dependent G2 arrest mediator candidate...member 5 Sestrins SESN1 SESN2 SESN3 Sestrins 1, 2, or 3 Siah Siah E3 ubiquitin protein ligase 1 TSC2 Tuberous...

  3. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...Human Parts 3rd 3 59,576 Human Whole Genome sgRNA iBAR Library 140633 Knockout Human Wei 3rd 3 58,630 Human...SAM v1 - 3 plasmid system 1000000057 (Zeocin) 1000000074 (Puromycin) Activation Human Zhang 3rd 3 70,290...70,290 SAM v1 - 3 plasmid system 1000000075 (Puromycin) Activation Mouse Zhang 3rd 3 69,716 SAM v2 - 2 plasmid...177663, 177664, 177670 Inhibition Human Adamson 3rd ∼3 366 or 1,573 Activity-optimized genome-wide library...92380 (Set B) Activation Human Doench and Root 3rd 3–6 56,762 (Set A) 56,476 (Set B) Broad GPP activation...92384 (Set B) Activation Mouse Doench and Root 3rd 3–6 67,187 (Set A) 66,889 (Set B) Broad GPP inhibition...92386 (Set B) Inhibition Human Doench and Root 3rd 3–6 57,050 (Set A) 57,011 (Set B) Broad GPP inhibition...

  4. Genetic Code Expansion

    Type
    Collection
    ...nitroY/haloY-F5 PylRS M. alvus 3-nitrotyrosine (3-nitro-Y), 3-halotyrosine (3-halo-Y) Bacterial TAG Ryan ...nitroY/haloY-F5 PylRS M. alvus 3-nitrotyrosine (3-nitroY) and 3-halotyrosine (3-haloY) Bacterial Ryan Mehl... Ryan Mehl 85498 pDule-3-nitroTyrosine (5B) 3NY (5B) synthetase M. jannaschii 3-nitroTyrosine Bacterial...Ryan Mehl 85499 pDule2-3-nitroTyrosine (5B) 3NY (5B) synthetase M. jannaschii 3-nitroTyrosine Bacterial.... jannaschii 3-aminotyrosine Bacterial TAG Huiwang Ai 153558 pMAH-EcaYRS EcaYRS E. coli 3-aminotyrosine...Jason W Chin 174078 pDule-3-nitroTyrosine (A7) 3NY (A7) synthetase M. jannaschii 3-nitroTyrosine Bacterial...Ryan Mehl 174079 pDule2-3-nitroTyrosine (A7) 3NY (A7) synthetase M. jannaschii 3-nitroTyrosine Bacterial...

  5. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...nicks the non-target strand and generates a 3’ flap. The 3’ flap binds to the PBS of the pegRNA and the...Drosophila U6:3 pegRNA BbsI No Vermilion Norbert Perrimon 149546 pCFD5-NS Drosophila Drosophila U6:3 pegRNA ...repair (MMR) system. Alternatively, if the edited 3’ flap is excised, the target sequence remains unchanged...stability epegRNA — additional protection added to 3’ tail of pegRNA to prevent RNA degradation For more...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Bacteria ID Plasmid Gene/Insert...Insert Promoter Selectable Marker PI Publication Return to top Drosophila ID Plasmid Gene/Insert Promoter ...Promoter Selectable Marker PI Publication Return to top Plant ID Plasmid Gene/Insert Promoter Selectable Marker...

  6. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...contains four different phosphobinding domains: 14-3-3β, 14-3-3σ, the WW2 domain of NEDD4, and the WW2 domain...111881 pNAS1b split mCherry 14-3-3σ Plasmid 111880 pNAS1b split mCherry 14-3-3β Pooled Library 111708 Mode...111706 Mode #2 Library (14-3-3σ) Pooled Library 111705 Mode #2 Library (14-3-3β) Pooled Library 111704 ...reactions (SERIOHL-KILR, Barber et al., 2018). Figure 3: The library of ~110,000 phosphosites is subdivided...iSPI_pSer_Subpool#4 Pooled Library 188528 iSPI_pSer_Subpool#3 Pooled Library 188527 iSPI_pSer_Subpool#2 Pooled Library..._Subpool#1 Plasmid 112031 pNAS1b split mCherry 14-3-3β ctrl Plasmid 112030 pNAS1b split mCherry NEDD4 ...the most abundant forms of posttranslational modification in cells and research into its many roles in...

  7. CRISPR Guide

    Type
    Collection
    ...EQR variant 3' NGAG SpCas9 VQR variant 3' NGAN or NGNG xCas9 3' NG, GAA, or GAT SpCas9-NG 3' NG Staphylococcus...SP); SpCas9 3' NGG SpCas9 D1135E variant 3' NGG (reduced NAG binding) SpCas9 VRER variant 3' NGCG SpCas9...jejuni (CJ) 3' NNNNRYAC Neisseria meningitidis (NM) 3' NNNNGATT Streptococcus thermophilus (ST) 3' NNAGAAW...the table above, 3' and 5' indicate on which end of targeted sequence the PAM is located. The majority of...continue to anneal to the target DNA in a 3′ to 5′ direction. The location of any potential mismatches matters...double-strand break (DSB) within the target DNA, located ∼3–4 nucleotides upstream of the PAM sequence. The...Each library typically contains ∼3–6 gRNAs per gene to ensure modification of every target gene, so CRISPR...

  8. Plasmids for Stem Cell Research

    Type
    Collection
    ...cells to pluripotency. Cell Stem Cell. 2008 Sep 11. 3(3):346-53. Jaenisch Lentivirus Human Single polycistronic...reprogramming in mouse. Cell Stem Cell. 2008 Mar 6. 2(3):230-40. Hochedlinger MMLV-derived Retrovirus Mouse...gene expression signatures. Cell Res. 2011 Mar;21(3):518-29. Cheng piggyBac Mouse Doxycycline-inducible...by Defined Factors. Cell Stem Cell. 2012 Sep 7;11(3):373-86. Jaenisch Fibroblasts Hepatocytes Retroviral...defined factors. Nature. 2011 Jun 29;475(7356):390-3. Suzuki Pancreatic Exocrine Cells Beta-cells Adenoviral...spinal motor neurons. Cell Stem Cell. 2011 Sep 2;9(3):205-18. Eggan Fibroblasts Neural Precursor Cells ...dendritic cells. Sci Immunol. 2018 Dec 7;3(30). pii: 3/30/eaau4292. Pereira Fibroblasts Neurons Lentiviral...

  9. Ras Pathway

    Type
    Collection
    ...PI3K; Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic and regulatory subunits - Class I PIN1 Peptidylprolyl... AKT3 v-akt murine thymoma viral oncogene homolog 3 ALK Anaplastic lymphoma receptor tyrosine kinase ARHGAP35... factor receptor FLT3 Fms related tyrosine kinase 3 FNT FNTA FNTB Farnesyltransferase, CAAX box FOS FBJ...PDGFRB Platelet derived growth factor receptor PDPK1 3-phosphoinositide dependent protein kinase 1 PEBP1 ...cancer therapy. Downward J. Nat Rev Cancer. 2003 Jan;3(1):11-22. PubMed PMID: 12509763 . Do you have suggestions...: A1,A2: Catalytic subunit alpha B1,B2: non-catalytic subunit beta G1, G2, G3: non-catalytic subunit gamma... below. Color is used for clarity and does not indicate a specific relationship. The content and map for...

  10. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ... to prevent viral spread. As a Biosafety Level 3 (BSL-3) agent, SARS-CoV-2 requires specialized facilities...listed below: ID Plasmid Description PI 44965 NL4-3 mCherry Luciferase Lentiviral dual reporter vector...vesicular stomatitis virus G) are packaged as replication-defective virus particles with the SARS-CoV-2...protein is inefficient for pseudotyping; several modifications may enhance infectious particle production, ...particular viral spike protein (MERS, CoV-1, CoV-2), modification, or variant. ID Plasmid Description Mutations...

  11. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...Anti-Synaptotagmin-3 [N278/19R] Synaptotagmin-3 Mouse Mouse IgG2a 114477 Anti-TRPML3/Mucolipin-3 [N268/19R] TRPML3... victoria Mouse IgG2a 114493 Anti-NGL-3/LRRC4B [N51/6.2R] NGL-3/LRRC4B Rat Mouse IgG2a 114494 Anti-VGlut3...Rat Mouse IgG2a 114518 Anti-Neuroligin-3 [N110/29.4R] Neuroligin-3 Rat Mouse IgG2a 114519 Anti-RGS14 [N133...149452 Anti-Kirrel3, short isoform 3 [N320/48R] Kirrel3, short isoform 3 Mouse Mouse IgG2a 149453 Anti-Kv4.2...Bassoon Rat Mouse IgG2a 177471 Anti-Synapsin-3 [L125/121R] Synapsin-3 Human Mouse IgG2a 177472 Anti-Pan-Synapsin...Mouse Mouse IgG2a 206608 Anti-Synapsin-3 [L125/76R] Synapsin-3 Human Mouse IgG2a 206610 Anti-Cav1.3 Ca2...Mouse Mouse IgG2b 206652 Anti-Neuroligin-3 [N110/29R-2b] Neuroligin-3 Rat Mouse IgG2b 206653 Anti-HCN4 [N114...

  12. mTOR Pathway

    Type
    Collection
    ...PIK3R6 Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic and regulatory subunits - Class I PKC PKC...homolog GSK3 GSK3A GSK3B Glycogen synthase kinase 3 MDM2 MDM2 proto-oncogene mSin1 Also known as MAPKAP1...Li J, Kim SG, Blenis J. Cell Metab. 2014 Mar 4;19(3):373-9. doi: 10.1016/j.cmet.2014.01.001. PubMed PMID...cell and regulates the switch from anabolic to catabolic processes. Active mTORC1 promotes protein and ...energy-intensive processes. mTORC1 also blocks catabolic processes including autophagy and lysosome biogenesis... below. Color is used for clarity and does not indicate a specific relationship. The content for this ...Pathway Color is used for clarity and does not indicate a specific relationship. The content for this ...

  13. Zhang Lab CRISPR Page

    Type
    Collection
    ...) and need to be followed on the 3' end by a 3bp NGG PAM sequence. 3. SpCas9 alone: This plasmid contains...site sequence (20bp) and need to be followed on the 3' end by a 3bp NGG PAM sequence. We have found that...Targeted gene activation using SAM There are two sets of 3 vectors each available for mammalian endogenous gene...sequence (21-22bp) and need to be followed on the 3' end by NGRRT or NGRRN PAM sequences. We have found...Jiang W, Marraffini LA, Zhang F. Science . 2013 Jan 3. DOI: 10.1126/science.1231143. PubMed . DNA targeting... nuclease along with the guide RNA. For most applications, we recommend using Cas9 with the single guide...BbsI, and a pair of annealed oligos (design is indicated below) can be cloned scarlessly into the vector...

  14. Lentiviral Prep Service

    Type
    Collection
    ...Barcode Library Version 3 Ready-to-use lentiviral particles carrying version 3 of the CellTag barcoding...barcoding library. Version 3 of the Celltag library contains 5737 barcodes to combinatorially index cells for... Accessories for Activating Gene Expression Catalytically-dead Cas9 (dCas9) can be fused to a transactivator...

  15. Luciferase Plasmid Collection

    Type
    Collection
    ...sequence in the 3' UTR David Bartel 12179 pIS1 Renilla Insertion of target sequence in the 3' UTR David Bartel... pIS2 Renilla Insertion of target sequence in the 3' UTR David Bartel 101139 pCMV-IRES-Renilla Luciferase-IRES-Gateway-Firefly...Firefly Insertion of imperfect miRNA sequence in the 3' UTR Carl Novina 33154 pAC-Luc Firefly Creation of...Macho 136010 psi-CHECK3 Firefly, Renilla Insertion of 3' UTR sequences downstream of Renilla luciferase. Firefly... of applications. To learn more and plan your experiment, check the plasmid's original publication or ...bacteria to aquatic animals to insects. Luciferase catalyzes the oxidation of a substrate, leading to the emission...advantages and disadvantages depending on the application, and different luciferases require different ...

  16. Botman-Teusink Yeast FP Collection

    Type
    Collection
    ...GGTGACGGTGCTGGTTTA–3' RV 5'–TCGATGAATTCGAGCTCG–3' ID Plasmid Selectable Marker Tags Publication FP Overexpression...marker. ID Plasmid Gene/Insert Selectable Marker Publication Additional Resources Addgene Kits MoClo-YTK Yeast...

  17. University of Florida Serotype Testing Panel for the Eye and Brain

    Type
    Collection
    ...tyrosine-mutant AAV serotype vectors. Mol Ther . 2009 Mar;17(3):463-71. PMID: 19066593 Other citations include: Bogner...cell entry characteristics. Gene Ther . 2020 Apr;27(3-4):127-142. PMID: 31611639 AAV2(trpYF) When using ...Expressing Retinoschisin. Hum Gene Ther Clin Dev . Sep;26(3):165-76. PMID: 26390090 Other citations include: Scalabrino... any application Balanced Salt Solution (BSS) An ophthalmic solution suitable for any application TMN200...ordering information. ID Name Promoter Description Category PI 157970 pTR-UF11 chimeric CMV/Chicken Beta actin...When using the AAV2(Y444F) serotype in future publications, please acknowledge Arun Srivastava and cite...using the AAV2(trpYF) serotype in future publications, please acknowledge Arun Srivastava and cite Petrs-Silva...

  18. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...CRISPR-Cas nuclease specificity using truncated guide RNAs. Nat Biotechnol . 32(3):279-84. PMID: 24463574 Fujita..., which together separate the individual crRNAs. (3) The tracrRNA and Cas9 nuclease form a complex with...Multiprotein Complexes in Human Cells. Cell Rep . 13(3):621-33. PMID: 26456817 Fu Y, Sander JD, Reyon D, ...Control of Gene Repression and Activation. Cell . 159(3):647-6. PMID: 25307932 Gilbert LA, Larson MH, Morsut...Endonuclease of a Class 2 CRISPR-Cas System. Cell . 163(3):759-71. PMID: 26422227 Zetsche B, Heidenreich M, ...development of new applications. The first CRISPR papers described two main categories of genome edits. ... lower off-target cleavage frequency. Truncated gRNAs: Truncated gRNAs display less off-target activity...

  19. CRISPR Plasmids - Drosophila

    Type
    Collection
    ...S. pyogenes (PAM = NGG) 49410 pCFD3-dU6:3gRNA dU6:3 BbsI Injection or in vitro transcription Virmilion...Port 49411 pCFD4-U6:1_U6:3tandemgRNAs dU6:1 and dU6:3 BbsI Injection or in vitro transcription Virmilion...ID Plasmid Gene/Insert Promoter PI Publication Activate Catalytically dead dCas9 fused to a transcriptional...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled...introduce a double-strand break (DSB) at a specific location based on a gRNA-defined target sequence. DSBs ...than NHEJ. ID Plasmid Gene/Insert Promoter PI Publication Nick CRISPR/Cas nickase mutants introduce gRNA-targeted...repair (HDR). ID Plasmid Gene/Insert Promoter PI Publication Prime Edit Cas9 H840A nickase fused to a reverse...

  20. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ... fast indicator evolved for two-photon microscopy. Cell. 2022 Aug 16. pii: S0092-8674(22)00916-3. Francois...Mammals. Neuron. 2020 Jun 20. pii: S0896-6273(20)30432-3. Karl Deisseroth , INTRSECT 2.0 Project Calcium Expression...Mammals. Neuron. 2020 Jun 20. pii: S0896-6273(20)30432-3. Karl Deisseroth , INTRSECT 2.0 Project Calcium Mitochondrial...Chromosome Condensation. Curr Biol. 2018 Feb 5;28(3):444-451.e6. Takeharu Nagai Magnesium MagFRET-1 Mg2...accumulation in bacteria. Biotechnol Biofuels. 2008 Jun 3. 1(1):11. Wolf Frommer Maltose Green fluorescent MBP-based...Learning. Cell. 2020 Dec 12. pii: S0092-8674(20)31612-3. Lin Tian Serotonin Serotonin (5-HT) sensor psychLight...biosensor. Cell. 2021 Apr 8. pii: S0092-8674(21)00374-3. Lin Tian Serotonin Cilia-targeted serotonin sensor...
Showing: 1 - 20 of 563 results