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C-term_TEV_aMFx1 (4a)

Plasmid

#238903

Purpose

Plasmid insert contains the aMFx1 C-terminal tag sequence with the TEV cleavage site for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

Depositor

Joseph Brock

Article

Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

Insert

C-term_TEV_aMFx1

Use

Synthetic Biology

Available Since

Sept. 8, 2025

Availability

Academic Institutions and Nonprofits only

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C-term_C-myc (4a)

Plasmid

#238907

Purpose

Plasmid insert contains the C-myc C-terminal tag sequence for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

Depositor

Joseph Brock

Article

Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

Insert

C-term_C-myc

Use

Synthetic Biology

Available Since

Sept. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pCR197

Plasmid

#240643

Purpose

Expression of the tri-cistronic construct NES-AmNeonGreen-P2A-mTurquoise2-NLS-T2A-mScarlet-I-PTS1 in Exaiptasia diaphana; In vitro transcription of the same construct via SP6 polymerase

Depositor

John Pringle

Inserts

AIPGENE865 promoter
SP6 promoter
AmNeonGreen
mTurquoise2
mScarlet-I

Use

Gene expression and genomic integration in fish

Tags

NES (from Exaiptasia diaphana MEK2), NLS (from SV…

Mutation

Codon-optimized for Exaiptasia diaphana, amino ac…

Available Since

Aug. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pX330 - LRR1 Terminal CRISPR

Plasmid

#221705

Purpose

Cas9 targeting plasmid with gRNA specific for LRR1 N-terminus

Depositor

Aga Gambus

Article

Jones et al iScience. 2024 Jun 12;27(7):110260. doi:

Insert

TGTAGCTTCATCTCGCCCAA

Use

CRISPR

Promoter

Chicken Beta-actin

Available Since

Aug. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pCSIIbleo-H2B-iRFP

Plasmid

#214830

Purpose

Encoding nuclear-targeted iRFP713.

Depositor

Michiyuki Matsuda

Article

Jikko et al J Cell Sci. 2025 Jul 16:jcs.263779. doi:

Insert

iRFP with nucleus-targeting sequence

Use

Lentiviral

Tags

H2B (human histone 2B)

Expression

Mammalian

Promoter

EF-1α

Available Since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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pCDFDuet-NHis6NocM-emptyMCS2

Plasmid

#240399

Purpose

expresses N-His tagged NocM (acyl carrier protein) from Nodularia sp. 06071

Depositor

Emily Balskus

Article

Wang et al Biochemistry. 2025 May 20;64(10):2173-21

Insert

nocM

Tags

His

Expression

Bacterial

Mutation

M1 in native sequence not included in insert

Promoter

T7

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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pETDuet-solo-NHis6-NocO

Plasmid

#240400

Purpose

expresses N-His tagged NocO (diiron halogenase) from Nodularia sp. 06071

Depositor

Emily Balskus

Article

Wang et al Biochemistry. 2025 May 20;64(10):2173-21

Insert

nocO

Tags

His

Expression

Bacterial

Mutation

M1 of native sequence not included in insert

Promoter

T7

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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pUBC(k)-AT5G42765-mKOk

Plasmid

#224853

Purpose

Plasmid for expression of AT5G42765.1 coding sequence tagged with mKOk in plants

Depositor

Rebecca Roston

Article

LaBrant et al bioRxiv 2025.06.20.660776; doi: https://

Insert

AT5G42765.1 (AT5G42765 Mustard Weed)

Expression

Plant

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pUBC(k)-AT5G55530-mKOk

Plasmid

#224854

Purpose

Plasmid for expression of AT5G55530.1 coding sequence tagged with mKOk in plants

Depositor

Rebecca Roston

Article

LaBrant et al bioRxiv 2025.06.20.660776; doi: https://

Insert

AT5G55530.1 (AT5G55530 Mustard Weed)

Expression

Plant

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pUBC(k)-AT5G63060-mKOk

Plasmid

#224858

Purpose

Plasmid for expression of AT5G63060.1 coding sequence tagged with mKOk in plants

Depositor

Rebecca Roston

Article

LaBrant et al bioRxiv 2025.06.20.660776; doi: https://

Insert

AT5G63060.1 (AT5G63060 Mustard Weed)

Expression

Plant

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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