We narrowed to 8,596 results for: Set

1. pOCC240

   Plasmid

   #118895

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for rec. protein with N-terminal MBP tag, cleavable with 3C and C-terminal mCherry, cleavable with TEV and HIS6, cleavable with 3C

   Depositor

   Marc Bickle, PEPC MPI CBG

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-MBP-3C-NotI-ccdB-AscI-TEV-mCherry-3C-HIS6-stop-HindIII cassette
   

   Tags

   MBP, cleavable with 3C protease and mCherry, clea…

   Expression

   Insect

   Promoter

   polH

   Available Since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSBK.013

   Plasmid

   #187213

   Purpose

   Expresses retron Eco1 ncRNA v35 (long a1/a2, barcode 5 [ACG-CGT]), and Cas1 + Cas2

   Depositor

   Seth Shipman

   Article

   Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:

   Inserts

   Retron Eco1 ncRNA v35 (long a1/a2, barcode 5 [ACG-CGT])
   Cas1 + Cas2
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   T7/Lac

   Available Since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGS1034

   Plasmid

   #63796

   Purpose

   Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal CBP-3xFLAG-TEV-ProtA triple tag and a LEU2 selection marker

   Depositor

   Monika Golas, Bjoern Sander

   Article

   Lin et al Biochim Biophys Acta. 2015 Mar;1854(3):1

   Insert

   CBP-3xFLAG-TEV-Protein A followed by KlLEU2
   

   Use

   E. coli cloning vector

   Tags

   CBP-3xFLAG-TEV-Protein A

   Promoter

   none

   Available Since

   April 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAC8_GWs-Lox (VE5589)

   Plasmid

   #163769

   Purpose

   Transfer vector for gene expression to generate recombinant baculoviruses by homologous recombination. Contains expression cassette under the pH promoter.

   Depositor

   Arnaud Poterszman

   Article

   Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.

   Insert

   no insert
   

   Tags

   no tag

   Expression

   Insect

   Promoter

   PH

   Available Since

   Dec. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pOCC175

   Plasmid

   #118890

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and N-terminal monomeric GFP, cleavable with TEV

   Depositor

   Marc Bickle, PEPC MPI CBG

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-HIS6-MBP-3C-mGFP-TEV-NotI-ccdB-AscI-stop-HindIII cassette
   

   Tags

   HIS6-MBP, cleavable with 3C protease; mGFP, cleav…

   Expression

   Insect

   Promoter

   polH

   Available Since

   Jan. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAav-MCS-PQS2-3xHA

   Plasmid

   #84917

   Purpose

   rAAV-based template for genome engineering of protein C-termini containing PQS2 and 3xHA tags and a selection cassette

   Depositor

   Sven Eyckerman

   Article

   Vandemoortele et al Sci Rep. 2016 Jun 6;6:27220. doi: 10.103

   Insert

   LOX-PGK-NEO-LOX
   

   Use

   AAV

   Tags

   PQS2 3xHA

   Promoter

   mPGK

   Available Since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1_SNORD27h

   Plasmid

   #73066

   Purpose

   expression clone for human SNORD27 (C/D box snoRNA U27)

   Depositor

   Stefan Stamm

   Article

   Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

   Insert

   SNORD27 (SNORD27 Human)
   

   Tags

   no tag

   Expression

   Mammalian

   Mutation

   expression cassette for SNORD27

   Promoter

   CMV

   Available Since

   Dec. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET-30a(+)-Syk-tSH2-PM

   Plasmid

   #111271

   Purpose

   express murine Syk tandem SH2 domains (Ser 8 to Gln 264), with Y130E mutation to mimic phosphorylation, in E.coli strain Rosetta 2 (DE3)

   Depositor

   Carol Post

   Article

   Feng et al Phys Chem Chem Phys. 2016 Feb 17;18(8):5

   Insert

   murine Syk tandem SH2 domains (Ser 8 to Gln 264), with Y130E mutation to mimic phosphorylation (Syk Mouse)
   

   Expression

   Bacterial

   Mutation

   Y130E mutation to mimic phosphorylation

   Promoter

   T7 promoter

   Available Since

   July 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSH231-EF1-BLST-Cas9-VPR

   Plasmid

   #115147

   Purpose

   Safe harbor site 231 knock-in vector with BlastR-Cas9-VPR expression cassette

   Depositor

   Raymond Monnat, Michael Phelps

   Article

   Pellenz et al Hum Gene Ther. 2019 Jul;30(7):814-828. d

   Insert

   BlastR-P2A-Cas9-VPR
   

   Use

   CRISPR

   Tags

   SV40NLS

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. Arabidopsis thaliana Atm3 d80 pET21a+

    Plasmid

    #173045

    Purpose

    Expresses AtAtm3 in E.coli

    Depositor

    Douglas Rees

    Article

    Fan et al Elife. 2022 Mar 25;11. pii: 76140. doi:

    Insert

    Arabidopsis thaliana Atm3 (ABCB25 Mustard Weed)
    

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    with N-terminal 80 amino acids deletion

    Available Since

    Oct. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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