We narrowed to 6,905 results for: crispr cas9 plasmids

1. B-Sniper SpCas9

   Plasmid

   #207361

   Purpose

   Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-Sniper SpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   F539S, M763I, K890N, amino acids 1005-1013 replac…

   Promoter

   CBh

   Available since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. B-HeFSpCas9-A1060R

   Plasmid

   #207372

   Purpose

   Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-HeFSpCas9-A1060R
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   N497A, R661A, Q695A, K848A, Q926A, K1003A, amino …

   Promoter

   CBh

   Available since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. B-HeFSpCas9-A1003K

   Plasmid

   #207371

   Purpose

   Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-HeFSpCas9-A1003K
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   N497A, R661A, Q695A, K848A, Q926A, R1060A, amino …

   Promoter

   CBh

   Available since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. B-HeFSpCas9-A848K

   Plasmid

   #207369

   Purpose

   Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-HeFSpCas9-A848K
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   N497A, R661A, Q695A, Q926A, K1003A, R1060A, amino…

   Promoter

   CBh

   Available since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. B-HeFSpCas9-A661R

   Plasmid

   #207367

   Purpose

   Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-HeFSpCas9-A661R
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   N497A, Q695A, K848A, Q926A, K1003A, R1060A, amino…

   Promoter

   CBh

   Available since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. B-evoSpCas9-Q661R

   Plasmid

   #207366

   Purpose

   Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-evoSpCas9-Q661R
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   M495V, Y515N, K526E, amino acids 1005-1013 replac…

   Promoter

   CBh

   Available since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. B-evoSpCas9-N515Y

   Plasmid

   #207364

   Purpose

   Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-evoSpCas9-N515Y
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   M495V, K526E, R661Q, amino acids 1005-1013 replac…

   Promoter

   CBh

   Available since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. HypaR-SpCas9

   Plasmid

   #126757

   Purpose

   Expression plasmid for human codon-optimized increased fidelity Hypa2SpCas9 (without U6-sgRNA coding sequence, with silent mutations)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   Hypa2SpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   R661A, N692A, M694A, Q695A, H698A

   Promoter

   CBh

   Available since

   Sept. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. St1Cas9_LMD-9_sgRNA_pUC19

   Plasmid

   #110627

   Purpose

   U6-driven sgRNA expression plasmid for Streptococcus thermophilus CRISPR1 (St1Cas9 LMD-9).

   Depositor

   Yannick Doyon

   Article

   Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

   Insert

   Empty St1Cas9 LMD-9 sgRNA backbone
   

   Use

   CRISPR and Synthetic Biology

   Tags

   None

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   May 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBZ186-pU6-(BbsI)_CBh-sv40NLS-Cas9-NLS T2A-mCherry

    Plasmid

    #170182

    Purpose

    Mammalian expression vector for sgRNAs cloned into the BbsI sites and for expression of spCas9 linked to mCherry via a T2A peptide

    Depositor

    Hunter Fraser

    Article

    Zhao et al CRISPR J. 2022 Jan 24. doi: 10.1089/cris

    Insert

    mCherry
    

    Use

    Tags

    3XFlag and T2A

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    Jan. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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