We narrowed to 4,045 results for: golden gate

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CMV_AcrIIA4_cpGR2(GGS5)-ΔN64_Q65_E66

Plasmid

#246052

Purpose

Expression of AcrIIA4-cpGR2 hybrid in mammalian cells after Bubeck et al Nat Methods 2018; enables chemogenetic control of SpyCas9

Depositor

Dominik Niopek

Article

Brenker et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

Insert

AcrIIA4_cpGR2-ΔN64/Q65/E66

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Mutation

ΔN64/Q65/E66

Available Since

Dec. 15, 2025

Availability

Academic Institutions and Nonprofits only

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CMV_AcrIIA5_cpGR2(GGS5)-N77

Plasmid

#246046

Purpose

Expression of AcrIIA5-cpGR2 hybrid in mammalian cells; insertion before N77; enables chemogenetic control of SauCas9

Depositor

Dominik Niopek

Article

Brenker et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

Insert

AcrIIA5_cpGR2-N77

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Available Since

Dec. 15, 2025

Availability

Academic Institutions and Nonprofits only

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CMV_AcrIIA5_cpGR2(GGS5)-D41

Plasmid

#246045

Purpose

Expression of AcrIIA5-cpGR2 hybrid in mammalian cells; insertion before D41; enables chemogenetic control of SauCas9

Depositor

Dominik Niopek

Article

Brenker et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

Insert

AcrIIA5_cpGR2-D41

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Available Since

Dec. 15, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
CMV_AcrIIA5_AsLOV2-N77

Plasmid

#246044

Purpose

Expression of AcrIIA5-AsLOV2 hybrid in mammalian cells; insertion before N77; enables optogenetic control of SauCas9

Depositor

Dominik Niopek

Article

Brenker et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

Insert

AcrIIA5_AsLOV2-N77

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Available Since

Dec. 15, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
CMV_AcrIIA5_AsLOV2-D41

Plasmid

#246043

Purpose

Expression of AcrIIA5-AsLOV2 hybrid in mammalian cells; insertion before D41; enables optogenetic control of SauCas9

Depositor

Dominik Niopek

Article

Brenker et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

Insert

AcrIIA5_AsLOV2-D41

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Available Since

Dec. 15, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
LentiX_(loxPconDIAL-YB_TATA)-mCherry-HRasG12V-BGH_pKG3906

Plasmid

#246345

Purpose

loxPcon DIAL Reporter Lentivirus expressing mCherry-HRasG12V in the presence of ZFa

Depositor

Kate Galloway

Article

Kabaria et al Nat Biotechnol. 2025 Oct 13. doi: 10.103

Insert

mCherry-GS-HRasG12V (HRAS Human)

Use

Lentiviral and Synthetic Biology

Mutation

G12V

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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(203-bp-DIAL-YB_TATA)-Halo-p53-BGH_pKG2745

Plasmid

#246339

Purpose

203-bp DIAL Reporter Plasmid with YB_TATA expressing Halo-p53 in the presence of ZFa and editable by Cre recombinase

Depositor

Kate Galloway

Article

Kabaria et al Nat Biotechnol. 2025 Oct 13. doi: 10.103

Insert

Halo-p53 (Trp53 Mouse)

Use

Synthetic Biology

Expression

Mammalian

Mutation

WT

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pTW411b

Plasmid

#246583

Purpose

different combination of A. thaliana and N. tabacum Rubisco assembly factors

Depositor

Tina Wang

Article

Li et al Proc Natl Acad Sci U S A. 2025 Sep 16;12

Insert

NtRaf1, AtRaf2, NtRbcX2, AtBSD2, AtCpn60alpha, AtCpn60beta, AtCpn20 (CPN60B, CPN60A, CPN20 )

Expression

Bacterial

Available Since

Oct. 24, 2025

Availability

Academic Institutions and Nonprofits only

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L4860 VEGFR2 NTEVp chain (75S NTEVp mutant), VEGFR1 CTEVp chain with ZF6a in MGEV

Plasmid

#244188

Purpose

Multigene vector for constitutive expression of mNeonGreen, VEGFR2 NTEVp chain (75S NTEVp mutant), and VEGFR1 CTEVp chain with ZF6a

Depositor

Joshua Leonard

Article

Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158

Insert

mNeonGreen; VEGFR2 NTEVp chain with NTEVp 75S mutant; VEGFR1 CTEVp chain with ZF6 synTF

Use

Synthetic Biology

Tags

3xFLAG

Expression

Mammalian

Mutation

NTEVp_75S, CTEVp_190K

Promoter

hEF1a, CMV

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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L4866 IL-10Rb NTEVp chain, IL-10Ra CTEVp chain with ZF1a in MGEV

Plasmid

#244190

Purpose

Multigene vector for constitutive expression of mTagBFP2, IL-10Rb NTEVp chain with human IgG VH signal peptide, and IL-10Ra CTEVp chain with ZF1a

Depositor

Joshua Leonard

Article

Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158

Insert

mTagBFP2; IL-10Rb NTEVp chain with human IgG VH signal sequence; IL-10Ra CTEVp chain with ZF1 synTF

Use

Synthetic Biology

Tags

3xFLAG

Expression

Mammalian

Mutation

NTEVp_75S, CTEVp_190K

Promoter

hEF1a, CMV

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
L4864 IL-10Rb NTEVp chain, IL-10Ra CTEVp chain with ZF6a in MGEV

Plasmid

#244189

Purpose

Multigene vector for constitutive expression of mTagBFP2, IL-10Rb NTEVp chain with human IgG VH signal peptide, and IL-10Ra CTEVp chain with ZF6a

Depositor

Joshua Leonard

Article

Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158

Insert

mTagBFP2; IL-10Rb NTEVp chain with human IgG VH signal sequence; IL-10Ra CTEVp chain with ZF6 synTF

Use

Synthetic Biology

Tags

3xFLAG

Expression

Mammalian

Mutation

NTEVp_75S, CTEVp_190K

Promoter

hEF1a, CMV

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pX330_mouseH3.1

Plasmid

#244241

Purpose

Expresses SpCas9 and a sgRNA targeting the mouse histone H3.1 loci for knock-in.

Depositor

Kazuhiro Maeshima

Article

Minami et al Sci Adv. 2025 Mar 28;11(13):eadu8400. do

Insert

sgRNA targeting mouse histone H3.1 (H3c1 Mouse)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Oct. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pX330_H3.2

Plasmid

#244239

Purpose

Expresses SpCas9 and a sgRNA targeting the human histone H3.2 loci for knock-in.

Depositor

Kazuhiro Maeshima

Article

Minami et al Sci Adv. 2025 Mar 28;11(13):eadu8400. do

Insert

sgRNA targeting human histone H3.2 (H3C14, H3C15 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Oct. 7, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pKMW121_WT-Cas9_U6-entry

Plasmid

#244818

Purpose

Mammalian expression of WT Cas9 with Golden Gate-compatible sgRNA spacer cassette

Depositor

Jennifer Doudna

Article

Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

Insert

SpyCas9 (WT)

Use

CRISPR

Tags

3xFLAG-SV40 NLS and Nucleoplasmin NLS

Expression

Mammalian

Promoter

CAG, U6

Available Since

Sept. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pKMW493_U6-sgRNA-entry

Plasmid

#244823

Purpose

Mammalian expression of SpyCas9 single guide RNA with Golden Gate-compatible sgRNA spacer

Depositor

Jennifer Doudna

Article

Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

Insert

SpyCas9 single guide RNA

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Sept. 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pKMW122_SpG-Cas9_U6-entry

Plasmid

#244819

Purpose

Mammalian expression of SpG Cas9 with Golden Gate-compatible sgRNA spacer cassette

Depositor

Jennifer Doudna

Article

Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

Insert

SpG Cas9

Use

CRISPR

Tags

3xFLAG-SV40 NLS and Nucleoplasmin NLS

Expression

Mammalian

Mutation

D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R

Promoter

CAG, U6

Available Since

Sept. 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
LLP1011

Plasmid

#239933

Purpose

dCas9 without Stop codon as Level-0 part (Golden Gate cloning)

Depositor

Ryan Lister

Article

Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

Insert

dCas9

Use

CRISPR and Synthetic Biology

Mutation

N/A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
LLP1004

Plasmid

#239926

Purpose

sgRNA compatible with TCTP-SynPro-07 and CaMV 35S-SynPro-12 as Level-0 part (Golden Gate cloning)

Depositor

Ryan Lister

Article

Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

Insert

sgRNA

Use

Synthetic Biology

Mutation

N/A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
LLP1005

Plasmid

#239927

Purpose

sgRNA compatible with TCTP-SynPro-02 as Level-0 part (Golden Gate cloning)

Depositor

Ryan Lister

Article

Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

Insert

sgRNA

Use

Synthetic Biology

Mutation

N/A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
LLP1006

Plasmid

#239928

Purpose

sgRNA compatible with TCTP-SynPro-02 as Level-0 part (Golden Gate cloning)

Depositor

Ryan Lister

Article

Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

Insert

sgRNA

Use

Synthetic Biology

Mutation

N/A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
LLP994

Plasmid

#239916

Purpose

sgRNA-D compatible with TCTP-SynPro-01, TCTP-SynPro-16 and CaMV 35S-SynPro-11 as Level-0 part (Golden Gate cloning)

Depositor

Ryan Lister

Article

Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

Insert

sgRNA-D

Use

Synthetic Biology

Mutation

N/A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
LLP1001

Plasmid

#239923

Purpose

sgRNA compatible with TCTP-SynPro-09 and CaMV 35S-SynPro-03 as Level-0 part (Golden Gate cloning)

Depositor

Ryan Lister

Article

Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

Insert

sgRNA

Use

Synthetic Biology

Mutation

N/A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
LLP1002

Plasmid

#239924

Purpose

sgRNA compatible with TCTP-SynPro-09 and CaMV 35S-SynPro-10 as Level-0 part (Golden Gate cloning)

Depositor

Ryan Lister

Article

Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

Insert

sgRNA

Use

Synthetic Biology

Mutation

N/A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
LLP993

Plasmid

#239915

Purpose

sgRNA-C compatible with TCTP-SynPro-01, TCTP-SynPro-17 and CaMV 35S-SynPro-11 as Level-0 part (Golden Gate cloning)

Depositor

Ryan Lister

Article

Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

Insert

sgRNA-C

Use

Synthetic Biology

Mutation

N/A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
LLP995

Plasmid

#239917

Purpose

sgRNA-E compatible with TCTP-SynPro-04, TCTP-SynPro-06 and CaMV 35S-SynPro-03 as Level-0 part (Golden Gate cloning)

Depositor

Ryan Lister

Article

Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

Insert

sgRNA-E

Use

Synthetic Biology

Mutation

N/A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
LLP996

Plasmid

#239918

Purpose

sgRNA compatible with TCTP-SynPro-05 and CaMV 35S-SynPro-15 as Level-0 part (Golden Gate cloning)

Depositor

Ryan Lister

Article

Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

Insert

sgRNA

Use

Synthetic Biology

Mutation

N/A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
LLP997

Plasmid

#239919

Purpose

sgRNA compatible with TCTP-SynPro-05 and CaMV 35S-SynPro-07 as Level-0 part (Golden Gate cloning)

Depositor

Ryan Lister

Article

Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

Insert

sgRNA

Use

Synthetic Biology

Mutation

N/A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
LLP999

Plasmid

#239921

Purpose

sgRNA compatible with TCTP-SynPro-08 and CaMV 35S-SynPro-04 as Level-0 part (Golden Gate cloning)

Depositor

Ryan Lister

Article

Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

Insert

sgRNA

Use

Synthetic Biology

Mutation

N/A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
LLP998

Plasmid

#239920

Purpose

sgRNA compatible with TCTP-SynPro-06, TCTP-SynPro-13 and CaMV 35S-SynPro-10 as Level-0 part (Golden Gate cloning)

Depositor

Ryan Lister

Article

Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

Insert

sgRNA

Use

Synthetic Biology

Mutation

N/A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
LLP1000

Plasmid

#239922

Purpose

sgRNA compatible with TCTP-SynPro-08 and CaMV 35S-SynPro-04 as Level-0 part (Golden Gate cloning)

Depositor

Ryan Lister

Article

Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

Insert

sgRNA

Use

Synthetic Biology

Mutation

N/A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
LLP1007

Plasmid

#239929

Purpose

sgRNA compatible with TCTP-SynPro-04, TCTP-SynPro-11 and CaMV 35S-SynPro-08 as Level-0 part (Golden Gate cloning)

Depositor

Ryan Lister

Article

Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

Insert

sgRNA

Use

Synthetic Biology

Mutation

N/A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
LLP1008

Plasmid

#239930

Purpose

sgRNA compatible with TCTP-SynPro-10 as Level-0 part (Golden Gate cloning)

Depositor

Ryan Lister

Article

Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

Insert

sgRNA

Use

Synthetic Biology

Mutation

N/A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
LLP1009

Plasmid

#239931

Purpose

sgRNA compatible with TCTP-SynPro-10 as Level-0 part (Golden Gate cloning)

Depositor

Ryan Lister

Article

Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

Insert

sgRNA

Use

Synthetic Biology

Mutation

N/A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
proUBI10::VIH2-FL::FLAG-H741A

Plasmid

#238335

Purpose

proUBI10::VIH2-FL::FLAG-H741A ubiquitous expression in Arabidopsis plants

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Mustard Weed)

Tags

3x Flag-tag

Expression

Plant

Mutation

AtVIH2-H741A

Available Since

June 27, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDF_AaRaf1/Raf2/RbcX2/BSD2

Plasmid

#229507

Purpose

Expresses Anthoceros agrestis chaperones: Raf1,Raf2,RbcX2,BSD2

Depositor

Laura Gunn

Article

Oh et al Mol Plant. 2024 Dec 2;17(12):1833-1849.

Inserts

Rubisco accumulation factor 1
Rubisco accumulation factor 2
RbcX2
BSD2

Expression

Bacterial

Mutation

N terminus chloroplast transit peptide truncation

Promoter

T7

Available Since

June 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDF_AaRaf1/Raf2/RbcX1/BSD2

Plasmid

#229508

Purpose

Expresses Anthoceros agrestis chaperones: Raf1,Raf2,RbcX1,BSD2

Depositor

Laura Gunn

Article

Oh et al Mol Plant. 2024 Dec 2;17(12):1833-1849.

Inserts

Rubisco accumulation factor 1
Rubisco accumulation factor 2
RbcX1
BSD2

Expression

Bacterial

Mutation

N terminus chloroplast transit peptide truncation

Promoter

T7

Available Since

June 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pPSmF

Plasmid

#226912

Purpose

Minimal plasmid for Plant STARR-seq with enhancer candidates in forward orientation

Depositor

Stan Fields

Article

Jores et al Plant Cell. 2025 Jun 4;37(6):koaf084. do

Type

Empty backbone

Expression

Plant

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pPSmR

Plasmid

#226913

Purpose

Minimal plasmid for Plant STARR-seq with enhancer candidates in reverse orientation

Depositor

Stan Fields

Article

Jores et al Plant Cell. 2025 Jun 4;37(6):koaf084. do

Type

Empty backbone

Expression

Plant

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
proUBI10::VIH2-FL::FLAG-C696A

Plasmid

#238336

Purpose

proUBI10::VIH2-FL::FLAG-C696A ubiquitous expression in Arabidopsis plants

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Mustard Weed)

Tags

3x Flag-tag

Expression

Plant

Mutation

AtVIH2-C696A

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
proUBI10::VIH2-FL::FLAG-K219A-D292A

Plasmid

#238331

Purpose

proUBI10::VIH2-FL::FLAG-K219A-D292A ubiquitous expression in Arabidopsis plants

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Mustard Weed)

Tags

3x Flag-tag

Expression

Plant

Mutation

AtVIH2-K219A-D292A

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
proUBI10::VIH2-FL::FLAG-R372A-H373A

Plasmid

#238332

Purpose

proUBI10::VIH2-FL::FLAG-R372A-H373A ubiquitous expression in Arabidopsis plants

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Mustard Weed)

Tags

3x Flag-tag

Expression

Plant

Mutation

AtVIH2-AtVIH2-R372A-H373A

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
proUBI10::VIH2-FL::FLAG-Wild type

Plasmid

#238330

Purpose

proUBI10::VIH2-FL::FLAG-Wild type ubiquitous expression in Arabidopsis plants

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Mustard Weed)

Tags

3x Flag-tag

Expression

Plant

Mutation

AtVIH2-Wild type

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
EF1a-FXN-P2A-mRuby2(miRE.FF4)-TS.FF6x1-bGH

Plasmid

#235301

Purpose

ComMAND open-loop circuit regulating FXN-P2A-mRuby2 (therapeutically relevant gene)

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

FXN-P2A-mRuby2 (FXN Human)

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available Since

June 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
EF1a-FXN-P2A-mRuby2-bGH

Plasmid

#235300

Purpose

ComMAND base gene regulating FXN-P2A-mRuby2 (therapeutically relevant gene)

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

FXN-P2A-mRuby2 (FXN Human)

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available Since

May 30, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
EF1a-FXN-P2A-mRuby2(miRE.FF4)-TS.FF4x1-bGH

Plasmid

#235302

Purpose

ComMAND closed-loop circuit regulating FXN-P2A-mRuby2 (therapeutically relevant gene)

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

FXN-P2A-mRuby2 (FXN Human)

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available Since

May 30, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
EF1a-EGFP-FMRP-bGH

Plasmid

#235265

Purpose

ComMAND EGFP-FMRP base gene

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

EGFP-Fmr1 (Fmr1 Mouse)

Use

Synthetic Biology

Tags

FLAG

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
EF1a-EGFP(miRE.FF4)-FMRP-TS.FF6x1-bGH

Plasmid

#235266

Purpose

ComMAND EGFP-FMRP open-loop circuit

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

EGFP-Fmr1 (Fmr1 Mouse)

Use

Synthetic Biology

Tags

FLAG

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
EF1a-EGFP(miRE.FF4)-FMRP-TS.FF4x1-bGH

Plasmid

#235267

Purpose

ComMAND EGFP-FMRP closed-loop circuit

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

EGFP-Fmr1 (Fmr1 Mouse)

Use

Synthetic Biology

Tags

FLAG

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pMT1-13X-gRNA1-2(AtUBI10)-MTAP-LUC

Plasmid

#234370

Purpose

T-DNA vector for expression of the two protein components of the MoonTag system under the control of the Arabidopsis Ubi10 promoter; Luciferase under control of transactivated promoter by two gRNAs

Depositor

Michael Smanski

Article

Casas-Mollano et al Nucleic Acids Res. 2023 Jul 21;51(13):70

Inserts

NbGP41-sfGFP-VP64-GB1
Luciferase
dCas9-13XGP41
gRNA 1-1 and gRNA 1-2

Use

Synthetic Biology

Tags

GB1, GP41, and sfGFP

Expression

Plant

Promoter

Arabidopsis U6, Arabidopsis Ubi10, and MTAP1 (syn…

Available Since

April 17, 2025

Availability

Academic Institutions and Nonprofits only

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pMT1-24X-gRNA1-2(AtUBI10)-MTAP-LUC

Plasmid

#234372

Purpose

T-DNA vector for expression of the two protein components of the MoonTag system under the control of the Arabidopsis Ubi10 promoter; Luciferase under control of transactivated promoter by two gRNAs

Depositor

Michael Smanski

Article

Casas-Mollano et al Nucleic Acids Res. 2023 Jul 21;51(13):70

Inserts

NbGP41-sfGFP-VP64-GB1
Luciferase
dCas9-24XGP41
gRNA 1-1 and gRNA 1-2

Use

Synthetic Biology

Tags

GB1, GP41, and sfGFP

Expression

Plant

Promoter

Arabidopsis U6, Arabidopsis Ubi10, and MTAP1 (syn…

Available Since

April 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge

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