Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 5,049 results for: golden gate

Showing: 3701 - 3750 of 5049 results

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pPR-1_19E_LN5N

Plasmid

#250474

Purpose

GRASP module to assemble sPPR proteins for RNA editing: module 19E_LN5N

Depositor

Ian Small

Article

Dennis et al Nucleic Acids Res. 2025 Oct 28;53(20):gk

Insert

19E_LN5N

Use

Synthetic Biology

Available Since

Jan. 29, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pPR-1_19E_LD5T

Plasmid

#250473

Purpose

GRASP module to assemble sPPR proteins for RNA editing: module 19E_LD5T

Depositor

Ian Small

Article

Dennis et al Nucleic Acids Res. 2025 Oct 28;53(20):gk

Insert

19E_LD5T

Use

Synthetic Biology

Available Since

Jan. 29, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pPR-1_19E_LD5N

Plasmid

#250472

Purpose

GRASP module to assemble sPPR proteins for RNA editing: module 19E_LD5N

Depositor

Ian Small

Article

Dennis et al Nucleic Acids Res. 2025 Oct 28;53(20):gk

Insert

19E_LD5N

Use

Synthetic Biology

Available Since

Jan. 29, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pPR-1_19A_LN5T

Plasmid

#250471

Purpose

GRASP module to assemble sPPR proteins for RNA editing: module 19A_LN5T

Depositor

Ian Small

Article

Dennis et al Nucleic Acids Res. 2025 Oct 28;53(20):gk

Insert

19A_LN5T

Use

Synthetic Biology

Available Since

Jan. 29, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pPR-1_19A_LN5N

Plasmid

#250470

Purpose

GRASP module to assemble sPPR proteins for RNA editing: module 19A_LN5N

Depositor

Ian Small

Article

Dennis et al Nucleic Acids Res. 2025 Oct 28;53(20):gk

Insert

19A_LN5N

Use

Synthetic Biology

Available Since

Jan. 29, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pPR-1_19A_LD5N

Plasmid

#250468

Purpose

GRASP module to assemble sPPR proteins for RNA editing: module 19A_LD5N

Depositor

Ian Small

Article

Dennis et al Nucleic Acids Res. 2025 Oct 28;53(20):gk

Insert

19A_LD5N

Use

Synthetic Biology

Available Since

Jan. 29, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pPR-1_14E_LN5T

Plasmid

#250467

Purpose

GRASP module to assemble sPPR proteins for RNA editing: module 14E_LN5T

Depositor

Ian Small

Article

Dennis et al Nucleic Acids Res. 2025 Oct 28;53(20):gk

Insert

14E_LN5T

Use

Synthetic Biology

Available Since

Jan. 29, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pPR-1_14E_LN5N

Plasmid

#250466

Purpose

GRASP module to assemble sPPR proteins for RNA editing: module 14E_LN5N

Depositor

Ian Small

Article

Dennis et al Nucleic Acids Res. 2025 Oct 28;53(20):gk

Insert

14E_LN5N

Use

Synthetic Biology

Available Since

Jan. 29, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pPR-1_14E_LD5T

Plasmid

#250465

Purpose

GRASP module to assemble sPPR proteins for RNA editing: module 14E_LD5T

Depositor

Ian Small

Article

Dennis et al Nucleic Acids Res. 2025 Oct 28;53(20):gk

Insert

14E_LD5T

Use

Synthetic Biology

Available Since

Jan. 29, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pPR-1_14E_LD5N

Plasmid

#250464

Purpose

GRASP module to assemble sPPR proteins for RNA editing: module 14E_LD5N

Depositor

Ian Small

Article

Dennis et al Nucleic Acids Res. 2025 Oct 28;53(20):gk

Insert

14E_LD5N

Use

Synthetic Biology

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pPR-1_14A_LN5T

Plasmid

#250463

Purpose

GRASP module to assemble sPPR proteins for RNA editing: module 14A_LN5T

Depositor

Ian Small

Article

Dennis et al Nucleic Acids Res. 2025 Oct 28;53(20):gk

Insert

14A_LN5T

Use

Synthetic Biology

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pPR-1_14A_LN5N

Plasmid

#250462

Purpose

GRASP module to assemble sPPR proteins for RNA editing: module 14A_LN5N

Depositor

Ian Small

Article

Dennis et al Nucleic Acids Res. 2025 Oct 28;53(20):gk

Insert

14A_LN5N

Use

Synthetic Biology

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pPR-1_14A_LD5T

Plasmid

#250461

Purpose

GRASP module to assemble sPPR proteins for RNA editing: module 14A_LD5T

Depositor

Ian Small

Article

Dennis et al Nucleic Acids Res. 2025 Oct 28;53(20):gk

Insert

14A_LD5T

Use

Synthetic Biology

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pPR-1_14A_LD5N

Plasmid

#250460

Purpose

GRASP module to assemble sPPR proteins for RNA editing: module 14A_LD5N

Depositor

Ian Small

Article

Dennis et al Nucleic Acids Res. 2025 Oct 28;53(20):gk

Insert

14A_LD5N

Use

Synthetic Biology

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pPR-1_2E_LN

Plasmid

#250459

Purpose

GRASP module to assemble sPPR proteins for RNA editing: module 2E_LN

Depositor

Ian Small

Article

Dennis et al Nucleic Acids Res. 2025 Oct 28;53(20):gk

Insert

2E_LN

Use

Synthetic Biology

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pPR-1_2E_LD

Plasmid

#250458

Purpose

GRASP module to assemble sPPR proteins for RNA editing: module 2E_LD

Depositor

Ian Small

Article

Dennis et al Nucleic Acids Res. 2025 Oct 28;53(20):gk

Insert

2E_LD

Use

Synthetic Biology

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pPR-1_2A_LN5T

Plasmid

#250457

Purpose

GRASP module to assemble sPPR proteins for RNA editing: module 2A_LN5T

Depositor

Ian Small

Article

Dennis et al Nucleic Acids Res. 2025 Oct 28;53(20):gk

Insert

2A_LN5T

Use

Synthetic Biology

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pPR-1_2A_LN5N

Plasmid

#250456

Purpose

GRASP module to assemble sPPR proteins for RNA editing: module 2A_LN5N

Depositor

Ian Small

Article

Dennis et al Nucleic Acids Res. 2025 Oct 28;53(20):gk

Insert

2A_LN5N

Use

Synthetic Biology

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pPR-1_2A_LD5T

Plasmid

#250455

Purpose

GRASP module to assemble sPPR proteins for RNA editing: module 2A_LD5T

Depositor

Ian Small

Article

Dennis et al Nucleic Acids Res. 2025 Oct 28;53(20):gk

Insert

2A_LD5T

Use

Synthetic Biology

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pPR-1_2A_LD5N

Plasmid

#250454

Purpose

GRASP module to assemble sPPR proteins for RNA editing: module 2A_LD5N

Depositor

Ian Small

Article

Dennis et al Nucleic Acids Res. 2025 Oct 28;53(20):gk

Insert

2A_LD5N

Use

Synthetic Biology

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pPR-1_1E_LN5T

Plasmid

#250453

Purpose

GRASP module to assemble sPPR proteins for RNA editing: module 1E_LN5T

Depositor

Ian Small

Article

Dennis et al Nucleic Acids Res. 2025 Oct 28;53(20):gk

Insert

1E_LN5T

Use

Synthetic Biology

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pPR-1_1E_LN5N

Plasmid

#250452

Purpose

GRASP module to assemble sPPR proteins for RNA editing: module 1E_LN5N

Depositor

Ian Small

Article

Dennis et al Nucleic Acids Res. 2025 Oct 28;53(20):gk

Insert

1E_LN5N

Use

Synthetic Biology

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pPR-1_1E_LD5T

Plasmid

#250451

Purpose

GRASP module to assemble sPPR proteins for RNA editing: module 1E_LD5T

Depositor

Ian Small

Article

Dennis et al Nucleic Acids Res. 2025 Oct 28;53(20):gk

Insert

1E_LD5T

Use

Synthetic Biology

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pPR-1_1E_LD5N

Plasmid

#250450

Purpose

GRASP module to assemble sPPR proteins for RNA editing: module 1E_LD5N

Depositor

Ian Small

Article

Dennis et al Nucleic Acids Res. 2025 Oct 28;53(20):gk

Insert

1E_LD5N

Use

Synthetic Biology

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pPR-1_1A_5T_AGGT

Plasmid

#250449

Purpose

GRASP module to assemble sPPR proteins for RNA editing: module 1A_5T_AGGT

Depositor

Ian Small

Article

Dennis et al Nucleic Acids Res. 2025 Oct 28;53(20):gk

Insert

1A_5T_AGGT

Use

Synthetic Biology

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pPR-1_1A_5N_AGGT

Plasmid

#250447

Purpose

GRASP module to assemble sPPR proteins for RNA editing: module 1A_5N_AGGT

Depositor

Ian Small

Article

Dennis et al Nucleic Acids Res. 2025 Oct 28;53(20):gk

Insert

1A_5N_AGGT

Use

Synthetic Biology

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pPR-1_1A_5N_AATG

Plasmid

#250446

Purpose

GRASP module to assemble sPPR proteins for RNA editing: module 1A_5N_AATG

Depositor

Ian Small

Article

Dennis et al Nucleic Acids Res. 2025 Oct 28;53(20):gk

Insert

1A_5N_AATG

Use

Synthetic Biology

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pTL53_pyCAG-s36GFPmNG-IRES-mCherry3NLS-polyA

Plasmid

#223545

Purpose

Minimal PUFFFIN cassette with s36GFP-mNeonGreen and mCherry-3NLS, separated by IRES, strong constitutive promoter CAG, no insulation or mammalian selection gene

Depositor

Sally Lowell

Article

Lebek et al EMBO J. 2024 Jul 12. doi: 10.1038/s44318

Insert

s36GFP-mNeonGreen, mCherry-3NLS

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 21, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pTL54_pyCAG-s36GFPmNG-t2A-mCherry3NLS-polyA

Plasmid

#223546

Purpose

Minimal PUFFFIN cassette with s36GFP-mNeonGreen and mCherry-3NLS, separated by t2A self-cleaving peptide, strong constitutive promoter CAG, no insulation or mammalian selection gene

Depositor

Sally Lowell

Article

Lebek et al EMBO J. 2024 Jul 12. doi: 10.1038/s44318

Insert

s36GFP-mNeonGreen, mCherry-3NLS

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 21, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
p-mCherry

Plasmid

#249067

Purpose

Expresses nucleus-localized mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.

Depositor

John Dueber, David Schaffer

Article

Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:

Insert

mCherry

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
p-mCherry-enCas9(D10A)

Plasmid

#249063

Purpose

Expresses nucleus-localized enCas9 (D10A) fused to mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.

Depositor

John Dueber, David Schaffer

Article

Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:

Insert

enCas9

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28-hisSumo-KE-Des27.7_F113L

Plasmid

#248016

Purpose

Expression of synthetic Kemp eliminase (Des27.7 F113L)

Depositor

Sarel Fleishman

Article

Listov et al Nature. 2025 Jul;643(8074):1421-1427. do

Insert

KE-Des27.7_F113L

Tags

12xHis-bdSumo

Expression

Bacterial

Mutation

F113L

Promoter

T7

Available Since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28-hisSumo-KE-Des27.7_noCoreStab

Plasmid

#248014

Purpose

Expression of synthetic Kemp eliminase (Ablation of Des27.7 ModularAssembly&PROSS&ActiveSite stability)

Depositor

Sarel Fleishman

Article

Listov et al Nature. 2025 Jul;643(8074):1421-1427. do

Insert

KE-Des27.7_noCoreStab

Tags

12xHis-bdSumo

Expression

Bacterial

Promoter

T7

Available Since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28-hisSumo-KE-Des27.7_ModularAssembly&activeSite

Plasmid

#248013

Purpose

Expression of synthetic Kemp eliminase (Ablation of Des27.7 ModularAssembly&ActiveSite design)

Depositor

Sarel Fleishman

Article

Listov et al Nature. 2025 Jul;643(8074):1421-1427. do

Insert

KE-Des27.7_ModularAssembly&activeSite

Tags

12xHis-bdSumo

Expression

Bacterial

Promoter

T7

Available Since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28-hisSumo-KE-Des27.7_PROSS

Plasmid

#248012

Purpose

Expression of synthetic Kemp eliminase (Ablation of Des27.7 ModularAssembly&PROSS)

Depositor

Sarel Fleishman

Article

Listov et al Nature. 2025 Jul;643(8074):1421-1427. do

Insert

KE-Des27.7_PROSS

Tags

12xHis-bdSumo

Expression

Bacterial

Promoter

T7

Available Since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28-hisSumo-KE-Des27.7_ModularAssembly

Plasmid

#248011

Purpose

Expression of synthetic Kemp eliminase (Ablation of Des27.7. ModularAssembly)

Depositor

Sarel Fleishman

Article

Listov et al Nature. 2025 Jul;643(8074):1421-1427. do

Insert

KE-Des27.7_ModularAssembly

Tags

12xHis-bdSumo

Expression

Bacterial

Promoter

T7

Available Since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28-hisSumo-KE-Des27

Plasmid

#247996

Purpose

Expression of synthetic Kemp eliminase (Des27)

Depositor

Sarel Fleishman

Article

Listov et al Nature. 2025 Jul;643(8074):1421-1427. do

Insert

KE-Des27

Tags

12xHis-bdSumo

Expression

Bacterial

Promoter

T7

Available Since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28-hisSumo-KE-Des27.3

Plasmid

#247999

Purpose

Expression of synthetic Kemp eliminase (Des27.3)

Depositor

Sarel Fleishman

Article

Listov et al Nature. 2025 Jul;643(8074):1421-1427. do

Insert

KE-Des27.3

Tags

12xHis-bdSumo

Expression

Bacterial

Promoter

T7

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28-hisSumo-KE-Des27.10

Plasmid

#248005

Purpose

Expression of synthetic Kemp eliminase (Des27.10)

Depositor

Sarel Fleishman

Article

Listov et al Nature. 2025 Jul;643(8074):1421-1427. do

Insert

KE-Des27.10

Tags

12xHis-bdSumo

Expression

Bacterial

Promoter

T7

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28-hisSumo-KE-Des27.1

Plasmid

#247997

Purpose

Expression of synthetic Kemp eliminase (Des27.1)

Depositor

Sarel Fleishman

Article

Listov et al Nature. 2025 Jul;643(8074):1421-1427. do

Insert

KE-Des27.1

Tags

12xHis-bdSumo

Expression

Bacterial

Promoter

T7

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28-hisSumo-KE-Des27.4

Plasmid

#248000

Purpose

Expression of synthetic Kemp eliminase (Des27.4)

Depositor

Sarel Fleishman

Article

Listov et al Nature. 2025 Jul;643(8074):1421-1427. do

Insert

KE-Des27.4

Tags

12xHis-bdSumo

Expression

Bacterial

Promoter

T7

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28-hisSumo-KE-Des27.12

Plasmid

#248007

Purpose

Expression of synthetic Kemp eliminase (Des27.12)

Depositor

Sarel Fleishman

Article

Listov et al Nature. 2025 Jul;643(8074):1421-1427. do

Insert

KE-Des27.12

Tags

12xHis-bdSumo

Expression

Bacterial

Promoter

T7

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28-hisSumo-KE-Des61.1

Plasmid

#248010

Purpose

Expression of synthetic Kemp eliminase (Des61.1)

Depositor

Sarel Fleishman

Article

Listov et al Nature. 2025 Jul;643(8074):1421-1427. do

Insert

KE-Des61.1

Tags

12xHis-bdSumo

Expression

Bacterial

Promoter

T7

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28-hisSumo-KE-Des27.5

Plasmid

#248001

Purpose

Expression of synthetic Kemp eliminase (Des27.5)

Depositor

Sarel Fleishman

Article

Listov et al Nature. 2025 Jul;643(8074):1421-1427. do

Insert

KE-Des27.5

Tags

12xHis-bdSumo

Expression

Bacterial

Promoter

T7

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28-hisSumo-KE-Des61

Plasmid

#248009

Purpose

Expression of synthetic Kemp eliminase (Des61)

Depositor

Sarel Fleishman

Article

Listov et al Nature. 2025 Jul;643(8074):1421-1427. do

Insert

KE-Des61

Tags

12xHis-bdSumo

Expression

Bacterial

Promoter

T7

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28-hisSumo-KE-Des27.2

Plasmid

#247998

Purpose

Expression of synthetic Kemp eliminase (Des27.2)

Depositor

Sarel Fleishman

Article

Listov et al Nature. 2025 Jul;643(8074):1421-1427. do

Insert

KE-Des27.2

Tags

12xHis-bdSumo

Expression

Bacterial

Promoter

T7

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28-hisSumo-KE-Des27.6

Plasmid

#248002

Purpose

Expression of synthetic Kemp eliminase (Des27.6)

Depositor

Sarel Fleishman

Article

Listov et al Nature. 2025 Jul;643(8074):1421-1427. do

Insert

KE-Des27.6

Tags

12xHis-bdSumo

Expression

Bacterial

Promoter

T7

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28-hisSumo-KE-Des27.11

Plasmid

#248006

Purpose

Expression of synthetic Kemp eliminase (Des27.11)

Depositor

Sarel Fleishman

Article

Listov et al Nature. 2025 Jul;643(8074):1421-1427. do

Insert

KE-Des27.11

Tags

12xHis-bdSumo

Expression

Bacterial

Promoter

T7

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28-hisSumo-KE-Des27.7

Plasmid

#248003

Purpose

Expression of synthetic Kemp eliminase (Des27.7)

Depositor

Sarel Fleishman

Article

Listov et al Nature. 2025 Jul;643(8074):1421-1427. do

Insert

KE-Des27.7

Tags

12xHis-bdSumo

Expression

Bacterial

Promoter

T7

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28-hisSumo-KE-Des27.9

Plasmid

#248004

Purpose

Expression of synthetic Kemp eliminase (Des27.9)

Depositor

Sarel Fleishman

Article

Listov et al Nature. 2025 Jul;643(8074):1421-1427. do

Insert

KE-Des27.9

Tags

12xHis-bdSumo

Expression

Bacterial

Promoter

T7

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge

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