We narrowed to 12,512 results for: BASE

1. SpCas9-NRCH-APOBEC-nCas9-Ung

   Plasmid

   #198552

   Purpose

   Expresses human codon-optimized SpCas9-NRCH-APOBEC-nCas9-Ung and blasticidin resistance: EFS promoter-SpCas9-NRCH-APOBEC-nCas9-Ung-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

   Insert

   SpCas9-NRCH-APOBEC-nCas9-Ung
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-hA3A-BE3-Y130F

   Plasmid

   #113428

   Purpose

   Expresses hA3A-BE3-Y130F in mammalian cells

   Depositor

   Jia Chen

   Article

   Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

   Insert

   hA3A-BE3-Y130F (APOBEC3A Human, S. pyogenes and Bacteriophage PBS2)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   hAPOBEC3A_Y130F

   Promoter

   CMV

   Available since

   Aug. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. CMV_Npu-BE3.9max N-terminal

   Plasmid

   #137171

   Purpose

   Expresses the N-terminal split-intein fragment of BE3.9max from the CMV promoter

   Depositor

   David Liu

   Article

   Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

   Insert

   Npu-BE3.9max_N-terminal
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Cas9 D10A

   Promoter

   CMV

   Available since

   Jan. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDicAID_nCas9-PmCDA-2A-NptII_ETR

   Plasmid

   #91695

   Purpose

   Dicot Target-AID vector expressing dicot-optimized nCas9-PmCDA1-2A-NptII with sgRNA targeting SlEtr

   Depositor

   Akihiko Kondo

   Article

   Shimatani et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

   Insert

   SpCas9
   

   Use

   Tags

   PmCDA1

   Expression

   Plant

   Mutation

   D10A for nickase Cas9

   Promoter

   PcUbi

   Available since

   June 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. CMV_Npu-ABEmax N-terminal

   Plasmid

   #137173

   Purpose

   Expresses the N-terminal split-intein fragment of ABEmax from the CMV promoter

   Depositor

   David Liu

   Article

   Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

   Insert

   Npu-ABEmax_N-terminal
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Cas9 D10A

   Promoter

   CMV

   Available since

   Jan. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLL4 sgRNA(MS2)-MS2-hA3a

   Plasmid

   #112130

   Purpose

   empty sgRNA cloning vector with MS2-humanA3a

   Depositor

   Fei-Long Meng

   Article

   Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

   Insert

   human Apobec3a (APOBEC3A Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6,CBh

   Available since

   Sept. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-dCpf1-BE-YEE

   Plasmid

   #107687

   Purpose

   Expresses dLbCpf1-BE-YEE in mammalian cells

   Depositor

   Jia Chen

   Article

   Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

   Insert

   dLbCpf1-BE-YEE
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   APOBEC1(W90Y/R126E/R132E), dLbCpf1(D832A/E925A/D1…

   Promoter

   CMV

   Available since

   April 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-BE3-FNLS(RA)

   Plasmid

   #112671

   Purpose

   CMV expression vector for BE3-FNLS construct (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   BE3-FNLS
   

   Use

   Tags

   3x FLAG

   Expression

   Mammalian

   Mutation

   NLS sequence at the N-terminus and D10A

   Promoter

   CMV

   Available since

   July 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLV-SI-112

   Plasmid

   #131127

   Purpose

   C-to-T base editing reporter

   Depositor

   Nozomu Yachie

   Article

   Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

   Insert

   Mutant EGFP
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   Sept. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMonAID_dCas9-PmCDA_Hyg_ALS

    Plasmid

    #91692

    Purpose

    Monocot Target-AID vector expressing rice-optimized dCas9-PmCDA1 with sgRNA targeting OsAls

    Depositor

    Akihiko Kondo

    Article

    Shimatani et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Insert

    SpCas9
    

    Use

    Tags

    PmCDA1

    Expression

    Plant

    Mutation

    D10A and H840A for dead Cas9

    Promoter

    2x35S

    Available since

    June 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-ACBEmax

    Plasmid

    #139106

    Purpose

    Mammalian ACBEmax expression vector (pSI-632)

    Depositor

    Nozomu Yachie

    Article

    Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

    Insert

    Bi_partite_NLS-TadA*-XTEN_linker-TadA-XTEN_linker-SpCas9(D10A)-SH3_linker-rAPOBEC1-2xUGI-SV40NLS
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    June 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. eA3A-BE3 (pRZ206)

    Plasmid

    #131315

    Purpose

    CAG promoter expression plasmid for hA3A(N57G)-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP.

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038

    Insert

    hA3A(N57G)-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBUN501

    Plasmid

    #50582

    Purpose

    CRISPR/Cas based plant genome editing and gene regulation; expresses zCas9D10A, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Bar resistance

    Depositor

    Qi-Jun Chen

    Article

    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

    Inserts

    zCas9D10A
    gRNA scaffold
    

    Use

    CRISPR; Plant expression

    Tags

    3xFLAG and NLS

    Expression

    Mutation

    Cas9D10A nickase, was derived from the zCas9 and …

    Promoter

    AtU6-26p and Ubi1p

    Available since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. GA-DEVD-GB-NES

    Plasmid

    #61020

    Purpose

    green ddFP based caspase-3 biosensor

    Depositor

    Robert Campbell

    Article

    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

    Insert

    ddGFP A and ddGFP B
    

    Use

    Tags

    HindIII and NES sequence LALKLAGLDIGS placed afte…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pCMV-dCpf1-BE-YE

    Plasmid

    #107686

    Purpose

    Expresses dLbCpf1-BE-YE in mammalian cells

    Depositor

    Jia Chen

    Article

    Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

    Insert

    dLbCpf1-BE-YE
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    APOBEC1(W90Y/R126E), dLbCpf1(D832A/E925A/D1148A)

    Promoter

    CMV

    Available since

    April 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. SPACE-ΔUGI (pRZ1836)

    Plasmid

    #140243

    Purpose

    CMV promoter expression plasmid for TadA*(V82G)-nCas9-pmCDA1(R187W)-P2A-EGFP

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

    Insert

    SPACE-ΔUGI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    V82G in TadA*, D10A in Cas9, R187W in pmCDA1

    Promoter

    CMV

    Available since

    June 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti-BE4RA-P2A-Puro

    Plasmid

    #112672

    Purpose

    Lentivirus for constitutive expression of BE4RA in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    BE4RA
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    NLS sequence at the N-terminus and D10A

    Promoter

    EF1s

    Available since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. SECURE miniABEmax-K20A/R21A (pJUL1774)

    Plasmid

    #131312

    Purpose

    CMV promoter expression plasmid for bpNLS-TadA7.10(K20A/R21A)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS (miniABEmax with K20A/R21A mutations).

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038

    Insert

    bpNLS-TadA7.10(K20A/R21A)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSI-359

    Plasmid

    #131131

    Purpose

    gRNA expression vector for A-to-G base editing reporter

    Depositor

    Nozomu Yachie

    Article

    Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

    Insert

    A-to-G reporter activation gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6 promoter

    Available since

    Sept. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. p3s-ABE7.10

    Plasmid

    #113128

    Purpose

    expressing ABE7.10 (TadA-TadA*7.10-nCas9-NLS-HA), human codon optimized

    Depositor

    Jin-Soo Kim

    Article

    Ryu et al Nat Biotechnol. 2018 Jul;36(6):536-539.

    Insert

    ABE 7.10
    

    Use

    Tags

    HA epitope and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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