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phBG-P53DD

Plasmid

#149707

Purpose

In vitro transcription of dominant negative form of P53 mRNA for RNA transfection into mammalian cells

Depositor

Erika Sasaki

Article

Watanabe et al Genes Cells. 2019 Jul;24(7):473-484. doi

Insert

Trp53 (Trp53 Mouse)

Use

Rna transcription

Tags

Expression

Mutation

deleted amino acid 14-301

Promoter

T7

Available since

June 24, 2020

Availability

Academic Institutions and Nonprofits only

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mature ISG15 C-term. domain (79-157)

Plasmid

#110764

Purpose

Bacterial expression of proISG15 C-term. domain

Depositor

David Komander

Article

Swatek et al Proc Natl Acad Sci U S A. 2018 Mar 6;115

Insert

ISG15 (ISG15 Human)

Use

Tags

N-His6-3C

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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pPB CAG Cdk6 R31C-IRES-EGFP

Plasmid

#153946

Purpose

piggyBac transposon vector with CAG promoter expressing Cdk6 (R31C mutant) and EGFP

Depositor

Luca Tiberi

Article

Ballabio et al Nat Commun. 2020 Jan 29;11(1):583. doi:

Insert

Cdk6 (Cdk6 Mouse)

Use

Tags

Expression

Mammalian

Mutation

R31C

Promoter

CAG

Available since

July 22, 2020

Availability

Academic Institutions and Nonprofits only

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proISG15 C-term. domain (79-165)

Plasmid

#110763

Purpose

Bacterial expression of proISG15 C-term. domain

Depositor

David Komander

Article

Swatek et al Proc Natl Acad Sci U S A. 2018 Mar 6;115

Insert

ISG15 (ISG15 Human)

Use

Tags

N-His6-3C

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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pPB CAG Gfi1:FLAG-IRES-EGFP

Plasmid

#153945

Purpose

piggyBac transposon vector with CAG promoter expressing FLAG-tagged Gfi1 and EGFP

Depositor

Luca Tiberi

Article

Ballabio et al Nat Commun. 2020 Jan 29;11(1):583. doi:

Insert

Gfi1 (Gfi1 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CAG

Available since

July 31, 2020

Availability

Academic Institutions and Nonprofits only

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mOrange-P2A-hKvb1

Plasmid

#154084

Purpose

Independent expression of mOrange and human Kvbeta 1 under human synapsin 1 promoter

Depositor

Michael Hoppa

Article

Cho et al Proc Natl Acad Sci U S A. 2020 Nov 24;11

Insert

mOrange-P2A-human Kvb1 (KCNAB1 Human, Synthetic)

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

human synapsin 1

Available since

Nov. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pCPF101-pfQC-WT

Plasmid

#184877

Purpose

expresses soluble plasmodium falciparum QC protein with a histag

Depositor

Ferenc Scheeren

Article

Kolli et al Proc Natl Acad Sci U S A. 2022 Aug 30;11

Insert

pfQC-wt

Use

Tags

His tag

Expression

Bacterial

Mutation

Promoter

Available since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

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pPB CAG SMARCA4 T910M-IRES-EGFP

Plasmid

#153949

Purpose

piggyBac transposon vector with CAG promoter expressing T910M mutant SMARCA4 (FLAG-tagged) and EGFP

Depositor

Luca Tiberi

Article

Ballabio et al Nat Commun. 2020 Jan 29;11(1):583. doi:

Insert

SMARCA4 (SMARCA4 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

T910M

Promoter

CAG

Available since

July 22, 2020

Availability

Academic Institutions and Nonprofits only

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pKI-cjACTB-EGFP-5'3'short

Plasmid

#129750

Purpose

Gene targeting in marmoset cells

Depositor

Hideyuki Okano

Article

Yoshimatsu et al Sci Rep. 2019 Feb 6;9(1):1528. doi: 10.1

Insert

ACTB (ACTB C. jacchus (common marmost))

Use

Marmoset targeting

Tags

EGFP

Expression

Mutation

Promoter

Available since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNAM-human eIF2C1-WT-Myc

Plasmid

#50360

Purpose

Expression of human eIF2C1 in mamalian cells

Depositor

Kumiko Ui-Tei

Article

Doi et al Curr Biol. 2003 Jan 8;13(1):41-6.

Insert

eIF2C1 (AGO1 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 4, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3-mPC1_4077-eGFP-3HA

Plasmid

#108390

Purpose

Express cytoplasmic tail mouse PC1 from amino acid 4077 to 4293

Depositor

Gregory Germino

Article

Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

Insert

Pkd1 (Pkd1 Mouse)

Use

Tags

eGFP-3HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mPC1_4119-3HA

Plasmid

#108395

Purpose

Express cytoplasmic tail mouse PC1 from amino acid 4119 to 4293

Depositor

Gregory Germino

Article

Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

Insert

Pkd1 (Pkd1 Mouse)

Use

Tags

3HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNAM-human eIF2C1-B-Myc

Plasmid

#50362

Purpose

Expression of human eIF2C1 mutant in mamalian cells

Depositor

Kumiko Ui-Tei

Article

Doi et al Curr Biol. 2003 Jan 8;13(1):41-6.

Insert

eIF2C1 (AGO1 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

contain PIWI domain

Promoter

CMV

Available since

Feb. 4, 2014

Availability

Academic Institutions and Nonprofits only
Strep-His-Tev-hCGNL1

Plasmid

#236520

Purpose

For expression of Paracingulin (human) in insect cells

Depositor

Sandra Citi

Article

Rouaud et al J Cell Biol. 2023 Jul 3;222(7):e20220806

Insert

CGNL1 (CGNL1 Human)

Use

Tags

2xStrep-10xHis-Tev

Expression

Insect

Mutation

WT

Promoter

Available since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-cCGNL1-Myc

Plasmid

#236516

Purpose

For expression of Paracingulin (dog) in mammalian cells

Depositor

Sandra Citi

Article

Rouaud et al J Cell Biol. 2023 Jul 3;222(7):e20220806

Insert

CGNL1 (CGNL1 Canis lupus familiaris (dog))

Use

Tags

GFP and Myc

Expression

Mammalian

Mutation

WT

Promoter

Available since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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pRRL-mouse STING #1-gRNA-Cas9-Puro

Plasmid

#186892

Purpose

gRNA targeting mouse STING (with Cas9 insert)

Depositor

Jonathan Kagan

Article

Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

Insert

Cas9 (Sting1 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pRRL-mouse STING #2-gRNA-Cas9-Puro

Plasmid

#186893

Purpose

gRNA targeting mouse STING (with Cas9 insert)

Depositor

Jonathan Kagan

Article

Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

Insert

Cas9 (Sting1 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pTRE3G-chimpanzee cGAS-HA

Plasmid

#186873

Purpose

Doxycycline-dependent expression of chimpanzee cGAS gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

Insert

Chimpanzee cGAS with C-terminal HA tag (CGAS Pan troglodytes)

Use

Retroviral

Tags

HA

Expression

Mutation

Promoter

TRE3G

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pTRE3G-mouse cGASdeltaN-HA

Plasmid

#186876

Purpose

Doxycycline-dependent expression of mouse cGAS gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

Insert

Mouse cGAS truncation mutant (148-508 a.a.) with C-terminal HA tag (Cgas Mouse, Synthetic)

Use

Retroviral

Tags

HA

Expression

Mutation

N-terminal truncation

Promoter

TRE3G

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pTRE3G-gibbon cGASdeltaN-HA

Plasmid

#186861

Purpose

Doxycycline-dependent expression of gibbon cGAS gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

Insert

Gibbon cGAS trucation mutant (152-514 a.a.) with C-terminal HA tag (CGAS Hylobatidae, Synthetic)

Use

Retroviral

Tags

HA

Expression

Mutation

N-terminal truncation

Promoter

TRE3G

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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