1,854 results

1. pHA847 - mec-4p::hrp-1mScarlet

   Plasmid

   #139201

   Purpose

   Expresses mScarlet tagged hrp-1 in mec-4 neurons

   Depositor

   Anne Hart

   Article

   Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

   Insert

   hrp-1 (hrp-1 Nematode)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   mScarlet added between RRMs and LC

   Promoter

   Available since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pHA848 - mec-4p::hrp-1HsLCWTmScarlet

   Plasmid

   #139202

   Purpose

   Expresses mScarlet tagged hrp-1HsLCWT in mec-4 neurons

   Depositor

   Anne Hart

   Article

   Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

   Insert

   hrp-1 (hrp-1 Nematode)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   mScarlet added between RRMs and LC, last exon rep…

   Promoter

   Available since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pHA849 - mec-4p::hrp-1HsLCD290VmScarlet

   Plasmid

   #139203

   Purpose

   Expresses mScarlet tagged hrp-1HsLCD290V in mec-4 neurons

   Depositor

   Anne Hart

   Article

   Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

   Insert

   hrp-1 (hrp-1 Nematode)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   mScarlet added between RRMs and LC, D290V, last e…

   Promoter

   Available since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pHA844 - hrp-1p::hrp-1mScarlet

   Plasmid

   #139204

   Purpose

   Expresses mScarlet tagged hrp-1

   Depositor

   Anne Hart

   Article

   Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

   Insert

   hrp-1 (hrp-1 Nematode)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   mScarlet added between RRMs and LC

   Promoter

   Available since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pHA845 - hrp-1p::hrp-1HsLCWTmScarlet

   Plasmid

   #139205

   Purpose

   Expresses mScarlet tagged hrp-1HsLCWT

   Depositor

   Anne Hart

   Article

   Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

   Insert

   hrp-1 (hrp-1 Nematode)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   mScarlet added between RRMs and LC, last exon rep…

   Promoter

   Available since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pHA846 - hrp-1p::hrp-1HsLCD290VmScarlet

   Plasmid

   #139206

   Purpose

   Expresses mScarlet tagged hrp-1HsLCD290V

   Depositor

   Anne Hart

   Article

   Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

   Insert

   hrp-1 (hrp-1 Nematode)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   mScarlet added between RRMs and LC, D290V, last e…

   Promoter

   Available since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. HRP-1LC

   Plasmid

   #139116

   Purpose

   express the LC of HRP-1

   Depositor

   Nicolas Fawzi

   Article

   Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

   Insert

   hrp-1 (hrp-1 Nematode)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   181-346

   Promoter

   Available since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pJ4M_HRP-1_FL

   Plasmid

   #139117

   Purpose

   express HRP-1 with a C-terminal maltose binding protein tag

   Depositor

   Nicolas Fawzi

   Article

   Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

   Insert

   hrp-1 (hrp-1 Nematode)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   None

   Promoter

   Available since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCFJ1820 - pDEST[2-3](5605)[Psmu-1 | smu-1 genomic | ccdB Cm]

   Plasmid

   #159838

   Purpose

   Three-fragment Gateway compatible pDEST vector to clone transgene downstream of smu-1 for germline expression from arrays

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   pDEST[2-3](5605)[Psmu-1 | smu-1 genomic | ccdB Cm]
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available since

   Nov. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pHJ4

    Plasmid

    #158785

    Purpose

    Pan-neuronal nuclear-localized GCaMP expression in C. elegans

    Depositor

    Jennifer Garrison, Zachary Knight

    Article

    Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

    Insert

    Prab-3::nls::GCaMP6m
    

    Use

    Tags

    NLS

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Sept. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pJW1884

    Plasmid

    #154332

    Purpose

    sgRNA Target Vector for CRISPR

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    cxTi10882 site targeting sgRNA (F+E) with R07E5.16 U6 promoter and 3'UTR
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    F+E mutations in sgRNA

    Promoter

    Available since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pJW1827

    Plasmid

    #154323

    Purpose

    Multi-cassette for SapTrap

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    30aa linker::mScarlet-I
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJW1841

    Plasmid

    #154338

    Purpose

    Promoter reporter

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    SV40 NLS::mScarlet_I(dpi)::-tbb-2 3'UTR
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Silent mutations to remove piRNA sites

    Promoter

    Available since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pJW1948

    Plasmid

    #154340

    Purpose

    Enhancer reporter

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    pes-10delta::SV40 NLS::mScarlet_I::PEST(dpi)::-tbb-2 3'UTR
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Silent mutations to remove piRNA sites

    Promoter

    Available since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDD289

    Plasmid

    #137018

    Purpose

    GFP-GLO^SEC^3xFlag vector with ccdB markers for cloning homology arms

    Depositor

    Daniel Dickinson, Bob Goldstein

    Insert

    GFP-GLO^SEC^3xFlag + ccdB
    

    Use

    CRISPR and Cre/Lox

    Tags

    3xFlag and C. elegans GLO-optimized GFP

    Expression

    Worm

    Mutation

    Promoter

    Available since

    April 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJW4.34

    Plasmid

    #131370

    Purpose

    expression of GST:MEG-4 in C. elegans

    Depositor

    Geraldine Seydoux

    Article

    Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

    Insert

    meg-4 (C36C9.1 Nematode)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDD376

    Plasmid

    #137022

    Purpose

    SapTrap mKate2-GLO donor

    Depositor

    Daniel Dickinson, Bob Goldstein

    Insert

    C. elegans GLO-optimized mKate2
    

    Use

    CRISPR; Saptrap donor for cloning repair templat…

    Tags

    C. elegans GLO-optimized mKate2

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pXF90

    Plasmid

    #139044

    Purpose

    MosSCI SapTrap assembly: Cassette 3 for 4-cassette system

    Depositor

    Erik Griffin

    Article

    Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

    Insert

    halotag::HDEL (includes STOP codon, PATC introns)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDK167

    Plasmid

    #134188

    Purpose

    Expression of C. elegans KNL1

    Depositor

    Iain Cheeseman

    Article

    Kern et al Mol Biol Cell. 2015 Jan 15;26(2):229-37.

    Insert

    KNL1 FL with oligomutant (knl-1 Nematode)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDK540

    Plasmid

    #134201

    Purpose

    Bacterial expression of C. elegans KNL1

    Depositor

    Iain Cheeseman

    Article

    Kern et al Mol Biol Cell. 2015 Jan 15;26(2):229-37.

    Insert

    KNL1 FL with YA mutant (knl-1 Nematode)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    YA

    Promoter

    Available since

    Feb. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge