Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 79,697 results for: ell

Showing: 1501 - 1520 of 79697 results

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pD649-HAsp-DLL4-Fc(DAPA)-AviTag-6xHis

Plasmid

#156889

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

DLL4 (DLL4 Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available Since

Jan. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-MCAM-Fc(DAPA)-AviTag-6xHis

Plasmid

#156909

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

MCAM (MCAM Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available Since

Jan. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pPB CAG SMARCA4-IRES-EGFP

Plasmid

#153948

Purpose

piggyBac transposon vector with CAG promoter expressing wild-type SMARCA4 (FLAG-tagged) and EGFP

Depositor

Luca Tiberi

Article

Ballabio et al Nat Commun. 2020 Jan 29;11(1):583. doi:

Insert

SMARCA4 (SMARCA4 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-KIR2DL4-Fc(DAPA)-AviTag-6xHis

Plasmid

#156662

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

KIR2DL4 (KIR2DL4 Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pD649-HAsp-CEACAM1-Fc(DAPA)-AviTag-6xHis

Plasmid

#156826

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

CEACAM1 (CEACAM1 Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pD649-HAsp-EDAR-COMP5AP-AviTag-9xHis

Plasmid

#157119

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

EDAR (EDAR Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pD649-HAsp-EDA2R-COMP5AP-AviTag-9xHis

Plasmid

#157096

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

EDA2R (EDA2R Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pTT3-ecdICAM1-ST3-His

Plasmid

#210666

Purpose

Expression of the extracellular domain of human ICAM1 fused to Spytag003 + Histag in HEK293 (or similar)

Depositor

Omer Dushek

Article

Patel et al EMBO J. 2024 Jan;43(1):132-150. doi: 10.

Insert

Extracellular domain of human ICAM-1 fused to Spytag003 and Histag (ICAM1 Human, Synthetic)

Tags

Histag and Spytag003

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-IL1R1-Fc(DAPA)-AviTag-6xHis

Plasmid

#156756

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

IL1R1 (IL1R1 Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pD649-HAsp-CD70-COMP5AP-AviTag-9xHis

Plasmid

#157113

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

CD70 (CD70 Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pD649-HAsp-IZUMO1-COMP5AP-AviTag-9xHis

Plasmid

#157279

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

IZUMO1 (IZUMO1 Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pD649-HAsp-IZUMO1-Fc(DAPA)-AviTag-6xHis

Plasmid

#156715

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

IZUMO1 (IZUMO1 Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pML333_LAMP1-C-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227898

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

LAMP1 homology arms with mEGFP-3xHA for insertion at C terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pML334_RAB7A-N-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227899

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

RAB7A homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pML531_TOMM20-C-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227890

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

TOMM20 homology arms with mEGFP-3xHA for insertion at C terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pD649-HAsp-SIGLEC7-Fc(DAPA)-AviTag-6xHis

Plasmid

#156773

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

SIGLEC7 (SIGLEC7 Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pML340_RAB11A-N-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227907

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

RAB11A homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pFUSE-rIgG-Fc2-ICAM1

Plasmid

#156462

Purpose

Expresses a rabbit Fc-ICAM1 fusion protein in mammalian cells

Depositor

Dirk Mielenz

Article

Reimer et al Cell Rep. 2020 Aug 11;32(6):108030. doi:

Insert

Intercellular Adhesion Molecule 1 (Icam1 Mouse)

Tags

ICAM1-rFc

Expression

Bacterial and Mammalian

Mutation

endogenous signal peptide replaced with IL2 signa…

Promoter

hEF1-HTLC

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-JAG2-Fc(DAPA)-AviTag-6xHis

Plasmid

#157027

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

JAG2 (JAG2 Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pML625_RTN4-N-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227895

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

RTN4 homology arms with mEGFP-3xHA for insertion at N terminus

Use

CRISPR

Available Since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 1501 - 1520 of 79697 results

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